Comparative analyses of polyphenolic composition of Fragaria spp. color mutants

Roy, Sutapa Mondal; Wu, Benhong; Liu, Wenwen; Archbold, Douglas D.

doi:10.1016/j.plaphy.2018.02.003

Cited by 14 publications

(20 citation statements)

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“…Using BS and PC berries from each development stage as an extraction (or biological) replicate, fruit were extracted using an acetone/water/acetic acid solution (70:29.5:0.5, v/v/v) and quantification of target phenolic compounds was carried out by high-performance liquid chromatography-mass spectrometry using external standards as described in Roy et al [8]. Three extraction replicates were analyzed per genotype by developmental stage.…”

Section: Fruit Extraction and Quantification Of Target Phenolic Compomentioning

confidence: 99%

“…The types and levels of these secondary metabolites vary among commercial strawberry cultivars (F. x ananassa Duch.) [2,3], as well as in the wild species F. chiloensis [4,5], F. pentaphylla [6] and F. vesca [7,8] The variation among cultivars is due to genotype, cultural practices, the production environment, and their interaction [9,10].…”

Section: Introductionmentioning

confidence: 99%

“…In recent years, the diploid woodland strawberry, Fragaria vesca (2n = 2x = 14), has become a popular research tool because it has a small genome (240 Mb) [11] a short generation time, and an available full genome sequence [12,13]. Besides the commonly found red-fruited genotypes of F. vesca, there are some white-fruited cultivars, some of which have been characterized as yellow, all lacking visible red color when ripe [8,14,15].…”

Section: Introductionmentioning

confidence: 99%

“…In addition, only the white-fruited F. vesca cultivar Yellow Wonder has been similarly characterized to date, but no others. We reported that PC differed from Yellow Wonder in flavonoid content when ripe [8], implying some differences in related gene expression patterns between them, so PC was chosen for this study.…”

Section: Introductionmentioning

confidence: 99%

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Developmental Variation in Fruit Polyphenol Content and Related Gene Expression of a Red-Fruited versus a White-Fruited Fragaria vesca Genotype

2018

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Two cultivars of F. vesca, red-fruited Baron Solemacher (BS) and white-fruited Pineapple Crush (PC), were studied to compare and contrast the quantitative accumulation of major polyphenols and related biosynthetic pathway gene expression patterns during fruit development and ripening. Developing PC fruit showed higher levels of hydroxycinnamic acids in green stages and a greater accumulation of ellagitannins in ripe fruit in comparison to BS. In addition to anthocyanin, red BS fruit had greater levels of flavan-3-ols when ripe than PC. Expression patterns of key structural genes and transcription factors of the phenylpropanoid/flavonoid biosynthetic pathway, an abscisic acid (ABA) biosynthetic gene, and a putative ABA receptor gene that may regulate the pathway, were also analyzed during fruit development and ripening to determine which genes exhibited differences in expression and when such differences were first evident. Expression of all pathway genes differed between the red BS and white PC at one or more times during development, most notably at ripening when phenylalanine ammonia lyase 1 (PAL1), chalcone synthase (CHS), flavanone-3′-hydroxylase (F3′H), dihydroflavonol 4-reductase (DFR), anthocyanidin synthase (ANS), and UDP:flavonoid-O-glucosyltransferase 1 (UFGT1) were significantly upregulated in the red BS fruit. The transcription factors MYB1 and MYB10 did not differ substantially between red and white fruit except at ripening, when both the putative repressor MYB1 and promoter MYB10 were upregulated in red BS but not white PC fruit. The expression of ABA-related gene 9-cis-epoxycarotenoid dioxygenase 1 (NCED1) was higher in red BS fruit but only in the early green stages of development. Thus, a multigenic effect at several points in the phenylpropanoid/flavonoid biosynthetic pathway due to lack of MYB10 upregulation may have resulted in white PC fruit.

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Section: Fruit Extraction and Quantification Of Target Phenolic Compomentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Developmental Variation in Fruit Polyphenol Content and Related Gene Expression of a Red-Fruited versus a White-Fruited Fragaria vesca Genotype

2018

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“…They may also affect fruit sensorial quality because anthocyanin composition will likely affect fruit colour intensity and hue. (38, 39).…”

Section: Discussionmentioning

confidence: 99%

Metabolite Quantitative Trait Loci for flavonoids provide new insights into the genetic architecture of strawberry (Fragariaxananassa) fruit quality

Labadie

Vallin

Petit

et al. 2020

Preprint

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28Flavonoids are products from specialized metabolism that contribute to fruit sensorial (colour) and 29 nutritional (antioxidant properties) quality. Here, using a pseudo full-sibling F1 progeny previously 30 studied for fruit sensorial quality of cultivated strawberry (Fragaria x ananassa), we explored over two 31 successive years the genetic architecture of flavonoid-related traits using LC-ESI-MS (13 compounds 32 including anthocyanins, flavonols and flavan-3-ols) and colorimetric assays (anthocyanins, flavonoids, 33 phenolics, FRAP and TEAC antioxidant capacity). Network correlation analysis highlighted the high 34 connectivity of flavonoid compounds within each chemical class and low correlation with colorimetric 35 traits except anthocyanins. Mapping onto the female and male linkage maps of 152 flavonoid metabolic 36 QTLs (mQTLs) and of 26 colorimetric QTLs indicated co-localization on few linkage groups of major 37 flavonoid-and taste-related QTLs previously uncovered. These results pave the way for the discovery of 38 genetic variations underlying flavonoid mQTLs and for marker-assisted selection of strawberry varieties 39 with improved sensorial and nutritional quality.40 41 Keywords. Fragaria x ananassa, fruit, specialized metabolism, flavonoids, anthocyanins, mQTL, colour, 42 antioxidant, nutritional quality 43 44 93 by Marker-Assisted Selection (MAS) of the sensorial and nutritional quality of strawberry fruit.94 95 Material and methods 96 Plant materials and preparation. 97A pseudo full-sibling F1 population of 165 individuals obtained from a cross between the variety 98 'Capitola' (CA75.121-101 x Parker, University of California, Davis, USA) and the advanced line 'CF1116' 99 (['Pajaro' x ('Earlyglow' x 'Chandler')], reference from Ciref, France) was developed. The two parents, 100'Capitola' and 'CF1116', display many contrasting fruit quality traits. (20,26). For each of the two 101 consecutive study years (2010 and 2011), six cold-stored strawberry plants per genotype, which were 102 planted in 2009 and 2010, were grown in soil-free pine bark substrate under plastic tunnel with daily 103 ferti-irrigation and control of biotic stresses. Mapping population included a total of 165 individuals over 104 the two study years. 105Within this progeny, 72 and 131 individuals including parents were respectively phenotyped in 2010 106 and in 2011. Fruits were harvested at red ripe stage, when red coloration of the fruit is homogeneous. 107To ensure conformity of the fruit samples and to avoid undesirable effects on antioxidant and fruit 108 polyphenolic contents, several precautions were taken. Fruits were collected in the morning and those 109 showing abnormal shape and size, or injuries were not harvested. For each genotype, two harvests of 4-110 8 fruits each were performed at the peak of fruit production (8-16 fruits in total). Fruits were 111 immediately frozen in liquid nitrogen and stored at -80°C, in order to avoid degradation of antioxidants 112 and polyphenolics. They were then ground into a fine po...

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Wild sweet cherry, strawberry and bilberry as underestimated sources of natural colorants and bioactive compounds with functional properties

et al. 2023

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Comparative analyses of polyphenolic composition of Fragaria spp. color mutants

Cited by 14 publications

References 30 publications

Developmental Variation in Fruit Polyphenol Content and Related Gene Expression of a Red-Fruited versus a White-Fruited Fragaria vesca Genotype

Developmental Variation in Fruit Polyphenol Content and Related Gene Expression of a Red-Fruited versus a White-Fruited Fragaria vesca Genotype

Metabolite Quantitative Trait Loci for flavonoids provide new insights into the genetic architecture of strawberry (Fragariaxananassa) fruit quality

Wild sweet cherry, strawberry and bilberry as underestimated sources of natural colorants and bioactive compounds with functional properties

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