Comparative analyses of the bacterial diversity on two different biodeteriorated wall paintings by DGGE and 16S rDNA sequence analysis

Gurtner, Claudia; Heyrman, Jeroen; Piñar, Güadalupe; Lubitz, Werner; Swings, Jean; Rölleke, Sabine

doi:10.1016/s0964-8305(00)00079-2

Cited by 117 publications

(85 citation statements)

References 41 publications

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“…Proteobacteria was the most representative phylum detected in these samples. Their presence have been previously reported and comprises common members of the bacterial community considering stone materials (Gurtner et al, 2000;Piñ ar et al, 2002;Akatova et al, 2007;Cappitelli et al, 2007). Stone monuments can represent extreme habitats, favouring the growth of a variety of specialized microorganisms.…”

Section: Discussionmentioning

confidence: 81%

“…Aliphatic hydrocarbons are examples of those sorts of organic compounds in building stones located in urban environments, which have been detected in samples from Seville Cathedral by Saiz-Jimenez (1993). The presence of this bacterium was reported in Lisbon and Seville samples, and also in samples derived from the wall paintings of Catherine Chapel in the Castle of Herberstein (Gurtner et al, 2000).…”

Section: Discussionmentioning

confidence: 94%

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Growth of phototrophic biofilms from limestone monuments under laboratory conditions

Miller

Láiz

Dionísio

et al. 2009

International Biodeterioration & Biodegradation

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a b s t r a c tIn the current study, five phototrophic biofilms from different Southern Europe limestone monuments were characterised by molecular techniques and cultivated under laboratory conditions. Phototrophic biofilms were collected from Orologio Tower in Martano (Italy), Santa Clara-a-Velha Monastery and Ajuda National Palace, both in Portugal, and Seville and Granada Cathedrals from Spain. The biofilms were grown under laboratory conditions and periodically sampled in order to monitor their evolution over a three-month period. Prokaryotic communities from natural samples and cultivated biofilms were monitored using denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S rRNA gene fragments in conjunction with clone sequencing and phylogenetic analysis. DNA-based molecular analysis of 16S rRNA gene fragments from the natural green biofilms revealed complex and different communities composition with respect to phototrophic microorganisms. The biofilms from Orologio Tower (Martano, Italy) and Santa Clara-a-Velha Monastery (Coimbra, Portugal) were dominated by the microalga Chlorella. The cyanobacterium Chroococcidiopsis was the dominating genus from Ajuda National Palace biofilm (Lisbon, Portugal). The biofilms from Seville and Granada Cathedrals (Spain) were both dominated by the cyanobacterium Pleurocapsa. The DGGE analysis of the cultivated biofilms showed that the communities developed differently in terms of species establishment and community composition during the threemonth incubation period. The biofilm culture from Coimbra (Portugal) showed a remarkable stability of the microbial components of the natural community in laboratory conditions. With this work, a multiple-species community assemblage was obtained for further stone colonisation experiments.

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Section: Discussionmentioning

confidence: 81%

Section: Discussionmentioning

confidence: 94%

Growth of phototrophic biofilms from limestone monuments under laboratory conditions

Miller

Láiz

Dionísio

et al. 2009

International Biodeterioration & Biodegradation

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“…In previous reports using PCR-DGGE, many results regarding the presence or predominance of microbes in the community differed depending upon the method used (González and Saiz-Jimenéz, 2005;Gurtner et al, 2000;Portillo et al, 2008;Schabereiter-Gurtner et al, 2002a, 2002b, 2004. However, in the case of the TT and KT samples, the major taxa in the microbial communities were mostly similar in these two methods.…”

Section: -1 the Upsides And Downsides Of Culture-dependent And Cultmentioning

confidence: 92%

“…Molecular community analyses (polymerase chain reaction-denaturing gradient gel electrophoresis [PCR-DGGE], clone library, and pyrosequence analyses) of the selected samples were used to clarify the microbial diversity of various biodeteriorated environments (Gurtner et al, 2000;Portillo et al, 2008Portillo et al, , 2009Saiz-Jiménez, 2003;Zengler, 2008). Details of our culture-independent methods (Fig.…”

Section: -4 Culture-independent (Molecular) Methods: Pcr-dgge Clonmentioning

confidence: 99%

“…Many studies have investigated the causes of biodeterioration of cultural heritage sites using cultureindependent methods, e.g., 16S rRNA gene sequence, PCR-DGGE, clone library, and NGS analyses Cheeptham, 2013;Engle, 2015;Gurtner et al, 2000;Li et al, 2016;Saiz-Jiménez, 2003, 2015Schabereiter-Gurtner et al, 2002a;Zengler, 2008). In particular, culture-independent methods have been used to analyze the microbial communities of the Lascaux Cave in France, the Altamira Cave in Spain, and the necropolis in Italy (Bastian et al, 2009a(Bastian et al, , 2009c(Bastian et al, , 2010Diaz-Herraiz et al, 2013;Garcia-Anton et al, 2014;Martin-Sanchez and Saiz-Jiménez, 2014;Saiz-Jiménez, 2014;SchabereiterGurtner et al, 2002b).…”

Section: -1 the Upsides And Downsides Of Culture-dependent And Cultmentioning

confidence: 99%

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Polyphasic insights into the microbiomes of the Takamatsuzuka Tumulus and Kitora Tumulus

Sugiyama¹,

Kiyuna²,

Nishijima³

et al. 2017

J. Gen. Appl. Microbiol.

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Microbial outbreaks and related biodeterioration problems have affected the 1300-year-old multicolor (polychrome) mural paintings of the special historic sites Takamatsuzuka Tumulus (TT) and Kitora Tumulus (KT). Those of TT are designated as a national treasure. The microbiomes of these tumuli, both located in Asuka village, Nara, Japan, are critically reviewed as the central subject of this report. Using culture-dependent methods (conventional isolation and cultivation), we conducted polyphasic studies of the these microbial communities and identified the major microbial colonizers (Fusarium spp., Trichoderma spp., Penicillium spp., dark Acremonium spp., novel Candida yeast spp., Bacillus spp., Ochrobactrum spp., Stenotrophomonas tumulicola, and a few actinobacterial genera) and noteworthy microbial members (Kendrickiella phycomyces, Cephalotrichum verrucisporum (∫ ∫ ∫ ∫ ∫Doratomyces verrucisporus), Sagenomella striatispora, Sagenomella griseoviridis, two novel Cladophialophora spp., Burgoa anomala, one novel species Prototheca tumulicola, five novel Gluconacetobacter spp., three novel Bordetella spp., and one novel genus and species Krasilnikoviella muralis) involved in the biodeterioration of mural paintings, plaster walls, and stone chamber interiors. In addition, we generated microbial community data from TT and KT samples using cultureindependent methods (molecular biological methods, including PCR-DGGE, clone libraries, and pyrosequence analysis). These data are comprehensively presented, in contrast to those derived from culture-dependent methods. Furthermore, the microbial communities detected using both methods are analytically compared, and, as a result, the complementary roles of these methods and approaches are highlighted. In related contexts, knowledge of similar biodeterioration problems affecting other prehistoric cave paintings, mainly at Lascaux in France and Altamira in Spain, are referred to and commented upon. Based on substrate preferences (or ecological grouping) and mapping (plotting detection sites of isolates), we speculate on the possible origins and invasion routes whereby the major microbial colonizers invaded the TT stone chamber interior. Finally, concluding remarks, lessons, and future perspectives based on our microbiological surveys of these ancient tumuli, and similar treasures outside of Japan, are briefly presented. A list of the microbial taxa that have been identified and fully or briefly described by us as known and novel taxa for TT and KT isolates since 2008 is presented in Supplementary Materials. 64SUGIYAMA et al.

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Sampling and Analysis Methods

Welker¹,

Nagarajan²,

Newberg³

2005

Contamination and ESD Control in High‐Technology Manufacturing

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Comparative analyses of the bacterial diversity on two different biodeteriorated wall paintings by DGGE and 16S rDNA sequence analysis

Cited by 117 publications

References 41 publications

Growth of phototrophic biofilms from limestone monuments under laboratory conditions

Growth of phototrophic biofilms from limestone monuments under laboratory conditions

Polyphasic insights into the microbiomes of the Takamatsuzuka Tumulus and Kitora Tumulus

Sampling and Analysis Methods

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