Comparative analyses of transcriptome and proteome in response to cotton bollworm between a resistant wild soybean and a susceptible soybean cultivar

Abstract

and four genes were significantly up-regulated in response to H. armigera in ED059 while down-regulated or not changed in cv. Tianlong 2. GO analyses showed the specifically expressed genes in ED059 were mainly enriched in metabolism-related pathways. The function of one potential resistant gene involved in the phenylpropanoid pathway was confirmed by use of transgenic Arabidopsis. Our study provided useful resources for the soybean improvement, which could contribute to the elucidation of defense mechanisms against chewing insects in plants.Keywords Soybean · Cotton bollworm · Transcriptome · Proteome · Defence Abstract Soybean is an important oil and protein crop in the world. Cotton bollworm (Helicoverpa armigera) is one of primary defoliating insects which seriously damage the soybean production. The wild soybean, ED059, was reported to be highly resistant to H. armigera. However, the molecular mechanism of resistance to H. armigera remains unknown. In this study, we conducted the transcript and protein profilings for ED059 and a susceptible soybean cultivar (cv.) Tianlong 2. In summary, 2213 genes and 116 proteins were specifically expressed in response to H. armigera in ED059, while 2179 genes and 9 proteins were specifically expressed in cv. Tianlong 2. Four hundred Xiaoyi Wang and Jianhua Lu have contributed equally to this work. Electronic supplementary materialThe online version of this article

Meta-Analysis and Multiomics of a Chromosome Segment Substitution Line Reveal Candidate Genes Associated with Seed Hardness in Soybean

“…25 The molecular mechanism of soybean defense against cotton bollworm was also explored by combined transcriptome-proteome analysis, and the function of a resistance gene was confirmed in transgenic Arabidopsis thaliana. 26 To date, there have been few reports on the use of combined omics analysis to explore soybean seed hardness. 27 In this study, we identified genes that showed differential expression between a hard and nonhard line at both the transcript and protein levels.…”

Regulated promoters applied to plant engineering: an insight over promising soybean promoters under biotic stress and their cis-elements

“…To identify putative cis-regulatory elements (CREs) commonly found in soybean gene promoters related to biotic stress, we selected 50 top-list upregulated genes using six transcriptome-wide expression data in which soybean was Regulated plant promoters and soybean biotic stress-related cis-elements 13-25 subjected to several pests, including: one virus (Soybean Mosaic Virus -SMV - (Zhang et al, 2019), two caterpillars (H. armigera and Lamprosema indicate - (Wang et al, 2017;Zeng et al, 2017), two fungi (Sclerotinia Sclerotiorum and Fusarium oxysporum - (Chang et al, 2019;Ranjan et al, 2019), and one nematode (M. javanica) (Beneventi et al, 2013). The gene list and their respective annotations are shown in Supplementary Table S1.…”

Transcriptome Analysis of Chinese Chestnut (Castanea mollissima Blume) in Response to Dryocosmus kuriphilus Yasumatsu Infestation

“…Genomic tools, such as transcriptome analysis, can aid in the identification of resistance genes and the development of blight-resistant chestnut [ 2 ]. High-throughput RNA-seq has been used to study genetic and molecular mechanisms underlying host plant resistance to pests [ 10 , 17 , 18 , 19 , 20 ]. Additionally, an investigation of the host transcriptome change in response to pests can facilitate the isolation of resistance genes, which can improve the efficiency of resistant varieties breeding by genetic engineering.…”