Comparative Analysis and Expression Patterns of the PLP_deC Genes in Dendrobium officinale

Zhang, Lei; Chen, Jiao; Cao, Yunpeng; Cheng, Xi; Wang, Jian; Jin, Qing; Cai, Yongping

doi:10.3390/ijms21010054

Cited by 5 publications

(4 citation statements)

References 51 publications

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“…In many Asian countries, the dried stem of Dendrobium officinale Kimura et Migo (DO) has been widely used as a herbal medicine for thousands of years [ 11 ]. About 1300 years ago, DO was reported as “one of the most famous Dendrobium in China”, due to its health benefits during the Tang dynasty [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Comparison of the Antioxidant Activities and Polysaccharide Characterization of Fresh and Dry Dendrobium officinale Kimura et Migo

et al. 2022

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It is generally believed that fresh Dendrobium officinale (FDO) has more significant pharmacological activity than dried Dendrobium officinale (DDO); however, the difference has not been clearly shown. Our study compared their antioxidant properties both in vitro and in vivo, and the molecular weight arrangement and monosaccharide composition of the fresh Dendrobium officinale polysaccharides (FDOPs) and the dried Dendrobium officinale polysaccharides (DDOPs) were analyzed by HPLC-GPC and GC-MS. The results showed that the FDO and its polysaccharides had more significant effects on scavenging DPPH, ABTS, and hydroxyl radicals than the DDO. In addition, both the FDO and DDO significantly reduced lipid peroxidation levels and increased the SOD, T-AOC, CAT, and GSH levels in mice with acute liver damage caused by CCl4, while the FDO and its polysaccharides were more effective. Histopathological analysis further verified the protective effect of the Dendrobium polysaccharides on CCl4-induced liver injury. The determination of the polysaccharides revealed that the polysaccharide and mannose contents of the FDO were significantly higher than their dried counterparts, and the homogeneous arrangement of the polysaccharides in the FDO was degraded into three polysaccharide fragments of different molecular weights in the DDO. Overall, our data identified differences in the antioxidant activities of the FDO and DDO, as well as the reasons for these differences.

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Section: Introductionmentioning

confidence: 99%

Comparison of the Antioxidant Activities and Polysaccharide Characterization of Fresh and Dry Dendrobium officinale Kimura et Migo

et al. 2022

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“…TPC and TFC are rich in plants, herbs, and flowers, which have antioxidative, anti‐inflammatory, and lipid‐lowering effects (Chandrasekara & Shahidi, 2018; Purohit et al., 2021). For example, the extracts of E Se tea, Que Zui tea, Anneslea fragrans leaves, or Dendrobium fimbriatum Hook have antioxidative, anti‐inflammatory, or hepatoprotective effects because of high TPC and TFC levels (Hu et al., 2023; Y. Wang et al., 2023; Z. Wang et al., 2023; Zhao, Dong, et al., 2023; Zhao, Zheng, et al., 2023). Furthermore, our previous studies illustrated that the polysaccharide of BFT had anti‐inflammatory effect via suppressing the NF‐κB pathway.…”

Section: Discussionmentioning

confidence: 99%

Acute, subacute, and sub‐chronic toxicity evaluation of Bletilla formosana tubers in rats

Wang,

Zhang,

Zhao

et al. 2024

Food Safety and Health

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Bletilla formosana (Hayata) Schltr. is an important economic medicinal and edible plant in China. Its tubers (BFTs) are traditionally consumed as a beverage and canned foods for promoting human health. In this paper, the nutrients, the contents of total phenol, flavonoids, and polysaccharides, and the safety and toxicity of BFT are determined. The chemical analysis reported that BFT is rich in proteins, polysaccharides, polyphenols, flavonoids, and trace elements. The acute (14 days), subacute (28 days), and sub‐chronic (90 days) toxicities of BFT were evaluated for the first time in female and male rats. An acute toxicity study showed that the female rats by single oral administrations of BFT with 10,000 mg/kg body weight (BW) did not have behavioral changes and mortality, indicating that 50% lethal dose (LD50) of BFT was over 10,000 mg/kg BW. Similarly, BFT at 5000 mg/kg BW for consecutive 28 and 90 days in rats was also not toxic for rats after the analysis of physiological and biochemical parameters. Additionally, there are no significant differences on the histopathological changes of the heart, liver, spleen, lung, kidney, ovary, or testis between the control and BFT groups. Our findings revealed that BFT with concentration of less than 5000 mg/kg BW are safe for the female or male rats, indicating that BFT could be regarded as a safe food source.

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“…The analysis of upstream elements and gene expression can provide a perspective for gene function speculation and functional differentiation research (Cao et al, 2019;Kong et al, 2019a;Zhang et al, 2020). We thus investigated the upstream elements of all the PLT genes in Gramineous crops and the PLT gene expression in various tissues of rice and maize at different developmental stages.…”

Section: Cis-elements In Upstream Promoters and Gene Expression Analymentioning

confidence: 99%

Micro-Evolution Analysis Reveals Diverged Patterns of Polyol Transporters in Seven Gramineae Crops

et al. 2020

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Polyol transporters (PLTs), also called polyol/monosaccharide transporters, is of significance in determining plant development and sugar transportation. However, the diverged evolutionary patterns of the PLT gene family in Gramineae crops are still unclear. Here a micro-evolution analysis was performed among the seven Gramineae representative crops using whole-genome sequences, i.e., Brachypodium distachyon (Bd), Hordeum vulgare (Hv), Oryza rufipogon (Or), Oryza sativa (Os), Sorghum bicolor (Sb), Setaria italica (Si), and Zea mays (Zm), leading to the identification of 12, 11, 12, 15, 20, 24, and 20 PLT genes, respectively. In this study, all PLT genes were divided into nine orthogroups (OGs). However, the number of PLT genes and the distribution of PLT OGs were not the same in these seven Gramineae species, and different OGs were also subject to different purification selection pressures. These results indicated that the PLT OGs of the PLT gene family have been expanded or lost unevenly in all tested species. Then, our results of gene duplication events confirmed that gene duplication events promoted the expansion of the PLT gene family in some Gramineous plants, namely, Bd, Or, Os, Si, Sb, and Zm, but the degree of gene family expansion, the type of PLT gene duplication, and the differentiation time of duplicate gene pairs varied greatly among these species. In addition, the sequence alignment and the internal repeat analysis of all PLTs protein sequences implied that the PLT protein sequences may originate from an internal repeat duplication of an ancestral six transmembrane helical units. Besides that, the protein motifs result highlighted that the PLT protein sequences were highly conserved, whereas the functional differentiation of the PLT genes was characterized by different gene structures, upstream elements, as well as co-expression analysis. The gene expression analysis of rice and maize showed that the PLT genes have a wide range of expression patterns, suggesting diverse biological functions. Taken together, our finding provided a perspective on the evolution differences and the functional characterizations of PLT genes in Gramineae representative crops.

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Comparative Analysis and Expression Patterns of the PLP_deC Genes in Dendrobium officinale

Cited by 5 publications

References 51 publications

Comparison of the Antioxidant Activities and Polysaccharide Characterization of Fresh and Dry Dendrobium officinale Kimura et Migo

Comparison of the Antioxidant Activities and Polysaccharide Characterization of Fresh and Dry Dendrobium officinale Kimura et Migo

Acute, subacute, and sub‐chronic toxicity evaluation of Bletilla formosana tubers in rats

Micro-Evolution Analysis Reveals Diverged Patterns of Polyol Transporters in Seven Gramineae Crops

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