1996
DOI: 10.1007/bf02173969
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Comparative analysis of 18 sex pheromone plasmids fromEnterococcus faecalis: detection of a new insertion element on pPD1 and implications for the evolution of this plasmid family

Abstract: A new IS element, IS1062, related to the enterococcal IS elements IS6770 and IS1252, was detected in the 3'-terminus of the surface exclusion gene, sep1, of sex pheromone plasmid pPD1 in Enterococcus faecalis. pPD1-bearing cells lack the surface exclusion function, probably as a consequence of this insertion. Analysis of pAD1 and pPD1 sequences (7.5 kb and 2.7 kb, respectively) downstream of their aggregation substance genes revealed no similarity in these DNA regions. Detailed DNA/DNA hybridization studies us… Show more

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Cited by 22 publications
(51 citation statements)
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“…The plasmids of the sex pheromone family in Enterococcus faecalis are among the most efficient bacterial conjugation systems known (16). The family consists of over 20 plasmids and shows extensive sequence homologies (28). E. faecalis strains can host several of these plasmids.…”
mentioning
confidence: 99%
“…The plasmids of the sex pheromone family in Enterococcus faecalis are among the most efficient bacterial conjugation systems known (16). The family consists of over 20 plasmids and shows extensive sequence homologies (28). E. faecalis strains can host several of these plasmids.…”
mentioning
confidence: 99%
“…There are at least three small ORFs carried on pAD1 which are not present on pCF10. While traE1 and orf1 are unique to pAD1 (and related cAD1-inducible plasmids) (15), orfY is ubiquitous for most sex pheromone plasmids, but at least in the special case of pCF10, it is disrupted by a stop codon, leaving only a truncated 198-bp ORF (prgT) (20). These ORFs might be responsible for pheromone specificity of induction.…”
Section: Resultsmentioning
confidence: 99%
“…Especially for two different plasmids, pAD1and pCF10, rather detailed data on the regulatory circuits are available. Surprisingly, despite a similar overall organization of the regulatory genes and highly homologous DNA regions (15), induction of aggregation substance seems to involve two mechanistically distinct strategies. While for the pAD1-encoded aggregation substance, Asa1, a trans-acting protein, TraE1, obviously serves as the general inducer of transcription (25,32) (a survey of the genetic data available for pAD1 is given in Fig.…”
mentioning
confidence: 98%
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“…Most pheromoneresponsive plasmids found in Enterococcus faecalis exhibit a narrow host range and transfer between E. faecalis strains at a high frequency (10 0 to 10 -2 per donor cell) within a few hours during broth mating. [11] The plasmids confer a mating response to a small peptide (i.e., a sex pheromone) secreted by potential recipient cells. This mating signal induces the synthesis of a surface aggregation substance that facilitates the formation of mating aggregates.…”
Section: Discussionmentioning
confidence: 99%