The electric field of transmission lines has serious negative impacts on residents’ production and life with the expansion of high voltage engineering. In order to study the influence of trees on the electric field of ultra-high voltage transmission lines, this paper conducted three-dimensional simulation calculations of the power frequency electric field of transmission lines based on the tree quantity and distribution. Firstly, in order to study the pattern of electric field strength distribution in transmission lines, the electric field strengths of transmission lines of different voltage levels were compared; the maximum-power-frequency electric field intensity of ultra-high voltage transmission lines occurs below the edge conductor. Secondly, by changing the number of trees, it was concluded that the electric field strength below the edge conductor gradually decreases with the number of trees. Finally, the maximum electric field strength value at 1.5 m below the edge conductor and the width of the transmission corridor decreased by changing the layout of the trees. The results show that studying the impact of a tree’s electromagnetic parameters on the power frequency electric field strength under transmission lines can help reduce the electric field strength and decrease the width of transmission corridors, which is of great significance for line design and cost savings.