Comparative analysis of barley leaf proteome as affected by drought stress

Abstract

The adaptive response of Egyptian barley land races to drought stress was analyzed using difference gel electrophoresis (DIGE). Physiological measurements and proteome alterations of accession number 15141, drought tolerant, and accession number 15163, drought sensitive, were compared. Differentially expressed proteins were subjected to MALDI-TOF-MS analysis. Alterations in proteins related to the energy balance and chaperons were the most characteristic features to explain the differences between the drought-tolerant and the drought-sensitive accessions. Further alterations in the levels of proteins involved in metabolism, transcription and protein synthesis are also indicated.

Influence of abiotic stresses on plant proteome and metabolome changes

“…Although such approach is correct and gives clues about differences in the response of such plants, the tolerance level is very often determined only according to physiological traits. Usually the following parameters are measured: relative water content (RWC), water loss rate (WLR) (WendelboeNelson and Morris 2012), value of fast chlorophyll fluorescence analysis (Ashoub et al 2013), photosynthesis rate, shoot and root length and fresh weight (Kausar et al 2013). Obviously, such analyses provide information about the differences in resistance to abiotic factors between the genotypes, but if the studied plant is a crop species, its crop yield should also be measured and taken into consideration while determining the tolerance level (Verslues et al 2006;Dolferus et al 2011).…”

“…The comparative analysis of barley leaves subjected to drought stress revealed the upregulation of HSP100, HSP90 in both sensitive and tolerant genotype. A member of HSP70 was found to be down-regulated only in the sensitive genotype (Ashoub et al 2013). In the study conducted by Kausar and co-workers, HSP70 was also down-regulated in drought-sensitive barley (Kausar et al 2013).…”

“…Much attention has been paid to proteomic studies on crop plants in recent years. Examples of researches on crop plants include, among others, barley (Ashoub et al 2013), maize (Pechanova et al 2013) rice (Lee et al 2009), wheat (Peng et al 2009) and soybean (Cheng et al 2010) subjected to various abiotic stresses. Especially, comparative proteomic studies based on analyzing contrasting plant genotypes in the stressed and non-stressed conditions are very informative and give insights into the plant reactions to environmental stimuli.…”

“…Transketolase, an enzyme involved in the Calvin cycle, showed a greater decrease in the accumulation profile in the sensitive cultivar. Glyoxysomal malate dehydrogenase and alanine glyoxylate aminotransferase, which are enzymes involved in photorespiration were increased, especially in the tolerant genotype suggesting a higher rate of photorespiration (Ashoub et al 2013;Aubry et al 2011). In another study concerning drought responses in barley, the following proteins were considered: RUBISCO-binding protein, RUBISCO activase and Photosystem I Reaction Centre II.…”

Drought Tolerant Wild Species Are the Important Sources of Genes and Molecular Mechanisms Studies: Implication for Developing Drought Tolerant Crops

“…In wheat and barley, transcript, protein, metabolite profi ling studies conducted in the last couple of years are shown in Table 17.3 . For example, changes in protein profi le of barley in response to drought stress were analyzed using a proteomics technique (Kausar et al 2013 ;Ashoub et al 2013 ). Alterations in proteins related to the energy balance and chaperons were the most characteristic features to explain the differences between the drought-tolerant and the drought-sensitive accessions.…”