Comparative analysis of biological phosphate removal (BPR) and non-BPR activated sludge bacterial communities with particular reference to Acinetobacter

Melasniemi, Hannes; Hernesmaa, Anne; Pauli, Anneli; Rantanen, Pirjo; Salkinoja‐Salonen, Mirja

doi:10.1038/sj.jim.2900591

Cited by 8 publications

(8 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These have concentrated mostly on isolates of the genus Acinetobacter because members of this genus are easily isolated from EBPR sludges (21,26,42) and some isolates show some characteristics that may be important to EBPR (16,36). However, evidence indicating that Acinetobacter may not be responsible for EBPR includes pure culture performances not correlating with biological models (7,38) and analyses of EBPR bacterial communities indicating that Acinetobacter microorganisms are not present in high enough numbers to account for EBPR (7,14,24,30,41). Investigations of other EBPR-associated microorganisms are limited, although there has been some interest in grampositive bacteria such as Microlunatus phosphovorus (33,39), the gram-negative Lampropedia (35), and the Actinobacteria and ␣ Proteobacteria (25).…”

mentioning

confidence: 99%

Identification of Polyphosphate-Accumulating Organisms and Design of 16S rRNA-Directed Probes for Their Detection and Quantitation

Crocetti

Hugenholtz

Bond

et al. 2000

Appl Environ Microbiol

663

530

View full text Add to dashboard Cite

Laboratory-scale sequencing batch reactors (SBRs) as models for activated sludge processes were used to study enhanced biological phosphorus removal (EBPR) from wastewater. Enrichment for polyphosphateaccumulating organisms (PAOs) was achieved essentially by increasing the phosphorus concentration in the influent to the SBRs. Fluorescence in situ hybridization (FISH) using domain-, division-, and subdivision-level probes was used to assess the proportions of microorganisms in the sludges. The A sludge, a high-performance P-removing sludge containing 15.1% P in the biomass, was comprised of large clusters of polyphosphate-containing coccobacilli. By FISH, >80% of the A sludge bacteria were ␤-2 Proteobacteria arranged in clusters of coccobacilli, strongly suggesting that this group contains a PAO responsible for EBPR. The second dominant group in the A sludge was the Actinobacteria. Clone libraries of PCR-amplified bacterial 16S rRNA genes from three high-performance P-removing sludges were prepared, and clones belonging to the ␤-2 Proteobacteria were fully sequenced. A distinctive group of clones (sharing >98% sequence identity) related to Rhodocyclus spp. (94 to 97% identity) and Propionibacter pelophilus (95 to 96% identity) was identified as the most likely candidate PAOs. Three probes specific for the highly related candidate PAO group were designed from the sequence data. All three probes specifically bound to the morphologically distinctive clusters of PAOs in the A sludge, exactly coinciding with the ␤-2 Proteobacteria probe. Sequential FISH and polyphosphate staining of EBPR sludges clearly demonstrated that PAO probe-binding cells contained polyphosphate. Subsequent PAO probe analyses of a number of sludges with various P removal capacities indicated a strong positive correlation between P removal from the wastewater as determined by sludge P content and number of PAO probe-binding cells. We conclude therefore that an important group of PAOs in EBPR sludges are bacteria closely related to Rhodocyclus and Propionibacter.

show abstract

mentioning

confidence: 99%

Identification of Polyphosphate-Accumulating Organisms and Design of 16S rRNA-Directed Probes for Their Detection and Quantitation

Crocetti

Hugenholtz

Bond

et al. 2000

Appl Environ Microbiol

663

530

View full text Add to dashboard Cite

show abstract

“…Vegetative growth and full-scale metabolism cease temporarily in sporulation and the fungus goes over to a stationary state with reduced metabolism. Reduced metabolism of the clustered PAO was reported by Beacham et al (1990) and reasons why polyP accumulation in BPR sludge, especially in a highly clustered PAO, can be expected to take place under stationary growth conditions were discussed by Melasniemi et al (1998).…”

Section: Established Pao Characteristics Match With Fungal Sporesmentioning

confidence: 99%

“…In addition to human excreta, grey waters and environmental runoff waters, there were no known sources of yeasts in the influent. The technical scale (42 m$) process with a conventional UCT configuration (for closer description, see Melasniemi et al, 1998) was run for several years as a pure BPR process, i.e. without the addition of any phosphate-precipitating chemicals.…”

Section: Methodsmentioning

confidence: 99%

“…A single dominant group of PAOs has been suggested to be present in BPR sludges treating genuine waste water due to the recurrent observations that the bulk of polyP is in a specific morphotype of micro-organism with big coccoid cells arranged in distinctive grape-like clusters (Fuhs & Chen, 1975 ;Buchan, 1981Buchan, , 1983Duncan et al, 1988 ;Beacham et al, 1990 ;Streichan et al, 1990 ;Jenkins et al, 1993 ;Melasniemi et al, 1998). This organism was initially associated with Acinetobacter, which set off a boom of research on this bacterium.…”

Section: Rationale Of the In Situ Approachmentioning

confidence: 99%

“…In a previous study (Melasniemi et al, 1998) it was shown that acinetobacters did not contain significant amounts of polyP in a UCT (University of Cape Town)-type nutrient-removal activated-sludge process treating municipal waste water. Instead, the stainable polyP in the sludge under process conditions was found almost exclusively in microbial cells corresponding to the descriptions of the grape-like clusters forming PAO so often seen to predominate in real waste water treating BPR processes (Fuhs & Chen, 1975 ;Buchan, 1981Buchan, , 1983Duncan et al, 1988 ;Beacham et al, 1990 ;Streichan et al, 1990 ;Jenkins et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Yeast spores seem to be involved in biological phosphate removal: a microscopic in situ case study

Melasniemi

Hernesmaa

2000

Microbiology

View full text Add to dashboard Cite

The principal polyphosphate-accumulating organism (PAO) in a biologicalphosphate-removal activated-sludge process was assessed microscopically. The organism was recognized by its distinct morphotype most easily after polyphosphate staining. The PAO occurred in large, homogeneous clusters. The cells of the PAO were the biggest cells abounding in the sludge -clearly bigger than average sludge bacteria. Typical of the principal PAO was a variation of cell size, even in fresh sludge. In acetate minimal medium containing ampicillin, the original principal PAO clusters were converted to clusters of clearly larger, polyphosphate-containing, vegetative yeast-like cells. Cycloheximide addition inhibited this and caused flock disintegration, disappearance of the principal PAO clusters and growth of free bacteria. The cell wall of the principal PAO was not of the usual bacterial character. It showed anomalous Gram staining, stained for chitin (not found in bacteria) and bound concanavalin A, like cell walls of many yeasts. In addition, the PAO cell wall was resistant to lysozyme, but sensitive to an enzyme mixture that lyses yeast cell walls. It was concluded that the principal PAO cells in the studied sludge were clustered spores of a yeast.

show abstract

Effect of pH change on the performance and microbial community of enhanced biological phosphate removal process

Zhang

Liu

Fang

2005

Biotechnol. Bioeng.

View full text Add to dashboard Cite

An acetate-rich wastewater, containing 170 mg/L of total organic carbon (TOC), 13 mg/L of N, and 15 mg/L of P, was treated using the enhanced biological phosphate removal (EBPR) process operated in a sequencing batch reactor. A slight change of pH of the mixed liquor from 7.0 to 6.5 led to a complete loss of phosphate-removing capability and a drastic change of microbial populations. The process steadily removed 94% of TOC and 99.9% of P from the wastewater at pH 7.0, but only 93% TOC and 17% of P 14 days after the pH was lowered to pH 6.5. The sludge contained 8.8% P at pH 7.0, but only 1.9% at pH 6.5. Based on 16S rDNA analysis, 64.8% of the clones obtained from the sludge at pH 7.0 were absent in the pH 6.5 sludge. The missing microbes, some of which were likely responsible for the phosphate removal at pH 7.0, included beta-Proteobacteria, Actinobacteria, Bacteriodetes/Chlorobi group, plus photosynthetic bacteria and Defluvicoccus of the alpha-Proteobacteria. Among them, the last two groups, which represented 9.3% and 10.1% of the EBPR sludge at pH 7.0, have rarely been reported in an EBPR system.

show abstract

Comparative analysis of biological phosphate removal (BPR) and non-BPR activated sludge bacterial communities with particular reference to Acinetobacter

Cited by 8 publications

References 21 publications

Identification of Polyphosphate-Accumulating Organisms and Design of 16S rRNA-Directed Probes for Their Detection and Quantitation

Identification of Polyphosphate-Accumulating Organisms and Design of 16S rRNA-Directed Probes for Their Detection and Quantitation

Yeast spores seem to be involved in biological phosphate removal: a microscopic in situ case study

Effect of pH change on the performance and microbial community of enhanced biological phosphate removal process

Contact Info

Product

Resources

About