2022
DOI: 10.3390/bios12090763
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Comparative Analysis of Cell Metabolic Activity Sensing by Escherichia coli rrnB P1-lux and Cd Responsive-Lux Biosensors: Time-Resolved Experiments and Mechanistic Modelling

Abstract: Whole-cell bacterial sensors are used in medical/environmental applications to detect chemicals, and to assess medium toxicity or stress. Non-specific constitutive biosensors generally serve the latter purpose, whereas chemical detection is performed with biosensors involving a specific chemical-inducible promoter. Herein, we show that functioning principles of specific and non-specific whole-cell biosensors are not exclusive as both can probe modulations of cell metabolic activity under stressing conditions. … Show more

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Cited by 4 publications
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“…Therefore, based on the traditional luminescent bacteria bioassay, two new detection methods are proposed. One involves using genetic engineering to transfer the structural genes responsible for luminescence to other bacteria and assemble them into recombinant luminescent bacteria, which can realize the detection of specific pollutants [27,28]. The other is to use magnetic nanomaterials and luminescent bacteria to assemble magnetic nanoparticles (MNPs)-based biosensors, which can be separated from the soil solution and tested under an external magnetic field, without centrifugation and other operations, to realize the detection of undisturbed soil [29].…”
Section: Introductionmentioning
confidence: 99%
“…Therefore, based on the traditional luminescent bacteria bioassay, two new detection methods are proposed. One involves using genetic engineering to transfer the structural genes responsible for luminescence to other bacteria and assemble them into recombinant luminescent bacteria, which can realize the detection of specific pollutants [27,28]. The other is to use magnetic nanomaterials and luminescent bacteria to assemble magnetic nanoparticles (MNPs)-based biosensors, which can be separated from the soil solution and tested under an external magnetic field, without centrifugation and other operations, to realize the detection of undisturbed soil [29].…”
Section: Introductionmentioning
confidence: 99%