Comparative analysis of cells and proteins of pumpkin plants for the control of fruit size

Nakata, Yumiko; Taniguchi, Go; Takazaki, Shinya; Oda-Ueda, Naoko; Miyahara, Katsunori; Ohshima, Yasumi

doi:10.1016/j.jbiosc.2012.04.005

Cited by 13 publications

(8 citation statements)

References 20 publications

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“…Frequency of cell division is critical to fruit morphogenesis in a variety species such as Tomato [30][31][32][33], melon [37], pumpkin [35], cucumber [36][37][38][39]. In present study, we found that the fruit tip shape in 186 cucumbers inbred lines also were a complex trait and exhibit significant variations with angle range from 99 to 173° and fruit tip index range from 1.4 to 3.5.…”

Section: Orientation Of Cell Division Is Critical To Fruit Tip Develosupporting

confidence: 48%

Diversity of cucumber fruit tip is attributed to the epistatic QTL pairs Ft4.1 and Ft6.1

Zhu¹,

Zhang²,

Liang³

et al. 2020

Preprint

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BackgroundThe shape of fruit tip is critical to appearance quality and commodity value of cucumber (Cucumis sativus L.). Although diversity of cucumber fruit tip is rich, the genetic basis and molecular mechanism were poorly understood. In this study, the morphological and histological features of fruit tip were investigated to accurately evaluate the diversity in cucumber germplasm. QTLs of fruit tip were genetically mapped by using F 2 and RILs populations. Candidate genes of major-effect QTLs were predicted. ResultsThe appearance of cucumber fruit tip was generally divided into sharp, oval-round and blunt-round categories, but morphological measurement showed that the angle of fruit tip was continuously distribute in range of 99~173° and the fruit tip index (the ratio of diameter and length of tips) was in range of 1.4~3.5. Histological analysis suggested that the different structures of fruit tips were owed to the different distribution of cell number in vertical and horizontal direction, rather than difference in cell size. In total, thirty fruit tip related QTLs were identified from two mapping populations including a F 2 population derived from EC1 (blunt round tip) × 8419s-1 (sharp tip) and a RILs population derived from IL52 (oval round tip) × CCMC (sharp tip). Two major-effect QTLs were detected, of which Ft4.

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Section: Orientation Of Cell Division Is Critical To Fruit Tip Develosupporting

confidence: 48%

Diversity of cucumber fruit tip is attributed to the epistatic QTL pairs Ft4.1 and Ft6.1

Zhu¹,

Zhang²,

Liang³

et al. 2020

Preprint

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“…In the tomato fruit and olive, the role of cell number in fruit variation was also demonstrated to occur at anthesis or before anthesis, increasing the range of fruit variation in combination with other traits, such as berry cell size and carpel number (Bertin et al 2003, Baldet et al 2006, Cong et al 2008, Rosati et al 2011. A recent study on pumpkin plants (Nakata et al 2012) which produced fruits weighing from one to several hundred kilograms found also that both the cell number and cell size were increased in a large fruit. These results suggest the importance of the combination of both mechanisms to increase significantly the size of the fruit between cultivars and are in agreement with our findings.…”

Section: Discussionmentioning

confidence: 98%

Genetic variability of berry size in the grapevine (Vitis vinifera L.)

Houel

Martin‐Magniette²,

Nicolas

et al. 2013

Australian Journal of Grape and Wine Research

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Background and Aims: Berry weight in the grapevine, as in the tomato, is variable, ranging from less than 1 g to 10 g. In the tomato, berry weight depends on cell number and volume but also on carpel number. The aim of this work was to decipher the range of variation and to propose a role for subtraits possibly underlying berry size variation in a highly diverse collection of cultivated grapevines. Methods and Results: Cell division before and after anthesis and cell expansion after anthesis appeared to be the major determinants of flesh weight variation between cultivars. Carpel number varied between cultivars, with two and three carpels per ovary. This trait, however, and also the seed weight did not clearly contribute to berry size variation, although a positive correlation was found between seed weight and number and berry weight at the intragenotypic level, in agreement with previous results. Conclusions: This work deciphered the main anatomical factors underlying variation in berry size in Vitis vinifera; they involved both common fleshy fruits factors but also specific vine factors. Significance of the Study: Further studies of the variation in berry size of the the grape will be assisted by the anatomical factors identified in this study.Keyswords: berry weight, carpel number, cell number and volume, fruit size, Vitis vinifera L. 208Berry size

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“…However, unfortunately, which genetic loci have been altered by breeding and selection from Hubbard to AG during the last century remains largely unclear. The only certainty is that polyploidy is not the reason for the large fruit formation of AG (Tatum et al, 2006;Nakata et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Whole-genome resequencing identified QTLs, candidate genes and Kompetitive Allele-Specific PCR markers associated with the large fruit of Atlantic Giant (Cucurbita maxima)

et al. 2022

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Atlantic Giant (AG) pumpkin (Cucurbita maxima) produces the world’s largest fruit. Elucidating the molecular mechanism of AG fruit formation is of scientific and practical importance. In this research, genome-wide resequencing of an F2 population produced by a cross between AG and its small-fruit ancestor Hubbard was used to identify quantitative trait loci (QTLs) and candidate genes. Transgressive segregation of fruit size-related traits was observed in the F2 population, suggesting that fruit size was a quantitative trait controlled by multiple genes. A genetic map with an average physical distance of 154 kb per marker was constructed, and 13 QTLs related to fruit size were identified using bin-map construction. RNA sequencing analysis revealed that pathways associated with assimilate accumulation into the fruit, including carbohydrate metabolism, were significantly enriched in differentially expressed genes. According to the predicted impact of mutation on the biological function of certain proteins, 13 genes were selected as candidate genes associated with fruit size, among which two phytohormone-related genes, CmaCh17G011340 (a flavin-containing monooxygenase) and CmaCh04G029660 (a leucine-rich repeat protein kinase) were chosen for further investigation. Finally, one insertion-deletion (inDel) and three single nucleotide polymorphisms (SNPs) were successfully transformed to Kompetitive Allele-Specific PCR (KASP) markers. The novel QTLs and candidate genes identified provide insights into the genetic mechanism of large fruit formation of AG, and the genetic map and tightly linked KASP markers developed in this study can be employed for marker-assisted breeding to alter fruit size of C. maxima.

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Comparative analysis of cells and proteins of pumpkin plants for the control of fruit size

Cited by 13 publications

References 20 publications

Diversity of cucumber fruit tip is attributed to the epistatic QTL pairs Ft4.1 and Ft6.1

Diversity of cucumber fruit tip is attributed to the epistatic QTL pairs Ft4.1 and Ft6.1

Genetic variability of berry size in the grapevine (Vitis vinifera L.)

Whole-genome resequencing identified QTLs, candidate genes and Kompetitive Allele-Specific PCR markers associated with the large fruit of Atlantic Giant (Cucurbita maxima)

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