Comparative Analysis of Chemical Constituents in Different Parts of Lotus by UPLC and QToF-MS

Pei, Haotian; Su, Wenyu; Gui, Meng Yang; Dou, Mingjie; Zhang, Yingxin; Wang, Cuizhu; Lu, Dan

doi:10.3390/molecules26071855

Cited by 24 publications

(18 citation statements)

References 31 publications

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“…The majority of these compounds have been identified before in other vegetal matrixes. In addition, it was checked that molecular formula and m/z matched [ 36 , 37 , 38 ]. Quinones are structures known for being responsible for the brown colour after an enzymatic oxidation of the fruit, so it could be thought that the avocado fruit was starting to suffer these reactions from the presence of chlorogenoquinones [ 39 ].…”

Section: Resultsmentioning

confidence: 99%

Biological Evaluation of Avocado Residues as a Potential Source of Bioactive Compounds

et al. 2022

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Avocado seed and peel are the main by-products from avocado industrialisation, and account for nearly 30% of fruit weight. Although they are usually discarded, their high phenolic content has been deeply associated with several nutritional and functional benefits. Thus, for a comprehensive analytical evaluation of both semi-industrial extracts, various steps have been developed: tentative characterisation and quantification of the phenolic composition using HPLC-ESI-qTOF-MS, determination of TPC and antioxidant activity by Folin–Ciocalteu, FRAP, TEAC and ORAC methods, evaluation of scavenging capacity against different ROS and measurement of the enzymatic inhibitory potential against potentially harmful enzymes. Finally, their bioactive potential was tested in a human platelet model where antiaggregatory activity was measured. Hence, 48 different compounds were identified, where flavonoids and procyanidins were the most representative groups. The higher TPC was found in avocado peel extract (190 ± 3 mg/g), which showed more antioxidant power and more capacity to decrease ROS generation than seed extract (60 ± 2 mg/g). In addition, both extracts showed enzymatic inhibition, especially against hyaluronidase, xanthine oxidase and acetylcholinesterase. Lastly, avocado peel was proven to inhibit platelet aggregation with significant results at 1, 0.75 and 0.5 mg/mL, where the extract showed reducing effects on agonists’ expression such as p-selectin or GPIIb/IIIa complex. These results demonstrate that both semi-industrial extracts—above all, avocado peel—have an interesting potential to be exploited as a natural by-product with antioxidant properties with multiple applications for the prevention of different pathologies.

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Section: Resultsmentioning

confidence: 99%

Biological Evaluation of Avocado Residues as a Potential Source of Bioactive Compounds

et al. 2022

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“…It is also rich in alkaloids, including coclaurine, 4′- N -methyl coclaurine, nuciferine, higenamine, higenamine 4- O -glucoside, and nelumboferine [ 93 , 94 , 95 , 96 , 97 ]. Pei et al detected 171 compounds from six different parts of the lotus in a recent study [ 69 ]. Higenamine was identified in the leaves and plumule.…”

Section: Resultsmentioning

confidence: 99%

Higenamine in Plants as a Source of Unintentional Doping

Kozhuharov

Ivanov

Ivanova

2022

Plants

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Background: Higenamine is a β2 agonist of plant origin. The compound has been included in WADA’s prohibited list since 2017. Higenamine may be detected in different plants and many food supplements of natural origin. Methods: Our literature search was conducted through PubMed, Science Direct, Google Scholar, and Web of Science studies investigating the presence of higenamine in plants that are used in traditional folk medicine or included in food supplements. Our study aimed to assess the risk of adverse analytical findings caused by higenamine-containing plants. Results: Based on our literature search, Nelumbo nucifera, Tinospora crispa, Nandina domestica, Gnetum parvifolium, Asarum siebodii, Asarum heterotropoides, Aconitum carmichaelii, and Aristolochia brasiliensis are higenamine-containing plants. Based on data from Eastern folk medicine, these plants can provide numerous health benefits. Professional athletes likely ingest these plants without knowing that they contain higenamine; these herbs are used in treatments for different conditions and various foods/food supplements in addition to folk medicine. Conclusion: Athletes and their teams must be aware of the issues associated with the use of plant-based products. They should avoid consuming higenamine-containing plants during and outside of competition periods.

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“…Therefore, the structure of analytes is efficiently confirmed through IM‐MS. In addition, to identify the other compounds in lotus plumule, the IM‐MS data were matched with relative references [25–27] and the Traditional Chinese Medicine database, and approximately 60 kinds of compounds were identified. The identified compounds are shown in Supporting information Table S1 [25–30].…”

Section: Resultsmentioning

confidence: 99%

Boron nitride nanosheet‐assisted matrix solid‐phase dispersion microextraction of alkaloids from lotus plumule by high‐performance liquid chromatography coupled with ultraviolet detection and ion mobility quadrupole time‐of‐flight mass spectrometry

Shi

Zhu

et al. 2021

Electrophoresis

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A boron nitride nanosheet (BNNS)‐assisted matrix solid‐phase dispersion method was established to microextract alkaloids from medicinal plants. The target compounds were identified by high‐performance liquid chromatography coupled with ultraviolet detection and ion mobility quadrupole time‐of‐flight mass spectrometry. During the experimental process, several important parameters, including the type of dispersant, the amount of dispersant, the grinding time, and the type of elution solvent, were optimized. Finally, the BNNSs were chosen as the best dispersant, and their microcosmic morphologies were identified by scanning electron microscopy and transmission electron microscopy. Because of the special property of BNNSs, the cost of this experiment was greatly reduced, especially in elution volume, sample amount (50 mg), and extraction time (2 min). Under the best conditions, 50 mg of sample powder was dispersed with 50 mg of BNNSs, the grinding time was 120 s, the mixed powder was eluted with 200 μL of methanol, and good linearity (r2 > 0.9993) and satisfactory recoveries (80–100%) were obtained. The inter‐ and intraday precisions were acceptable, with RSDs lower than 2.01 and 4.84%, respectively. The limits of detection ranged from 2.54 to 15.00 ng/mL, and the limits of quantitation were 8.47 to 50.00 ng/mL. The proposed method was successfully applied for the determination of liensinine, isoliensinine, and neferine in lotus plumule.

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Comparative Analysis of Chemical Constituents in Different Parts of Lotus by UPLC and QToF-MS

Cited by 24 publications

References 31 publications

Biological Evaluation of Avocado Residues as a Potential Source of Bioactive Compounds

Biological Evaluation of Avocado Residues as a Potential Source of Bioactive Compounds

Higenamine in Plants as a Source of Unintentional Doping

Boron nitride nanosheet‐assisted matrix solid‐phase dispersion microextraction of alkaloids from lotus plumule by high‐performance liquid chromatography coupled with ultraviolet detection and ion mobility quadrupole time‐of‐flight mass spectrometry

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