Comparative analysis of circulating hemocytes of the freshwater snail Pomacea canaliculata

Accorsi, Alice; Bucci, Laura; Eguileor, Magda de; Ottaviani, Enzo; Malagoli, Davide

doi:10.1016/j.fsi.2013.02.008

Cited by 47 publications

(71 citation statements)

References 29 publications

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“…Pc possesses small blast‐like hemocytes and large hemocytes with low nuclear/cytoplasmic ratio and either agranular or granular cytoplasm . Consistently, present electron microscopy results evidenced granular (GH) and agranular (AH) circulating hemocytes.…”

Section: Proteins Identified From Circulating Hemocytes Of Pc By Lc‐msupporting

confidence: 87%

“…Pc possesses small blast-like hemocytes and large hemocytes with low nuclear/cytoplasmic ratio and either agranular or granular cytoplasm. [6] Consistently, present electron microscopy results evidenced granular (GH) and agranular (AH) circulating hemocytes. More than 80% of GH displayed an electron-dense cytoplasm containing granules with different electron-density, potentially due to differences in enzyme content (Figure 1a-c).…”

Section: Doi: 101002/pmic201800314supporting

confidence: 83%

“…In Pc kidney, a role for agranular hemocytes in the mobilization of glycogen deposits has been hypothesized . In our experiments, circulating AH showed neither pseudopods nor granules (Figure d,e), and some of them are likely related with blast‐like hemocytes …”

Section: Proteins Identified From Circulating Hemocytes Of Pc By Lc‐mmentioning

confidence: 59%

“…The immune system of Pc is based on cellular, represented by hemocytes, and soluble components . Information exists about Pc hemocyte morphology and proliferation but no cell‐specific molecular data are currently available. Here we present, beside an ultrastructural overview, the first proteomic dataset on Pc circulating hemocytes.…”

Section: Proteins Identified From Circulating Hemocytes Of Pc By Lc‐mmentioning

confidence: 99%

“…about Pc hemocyte morphology [6][7][8][9] and proliferation [7] but no cell-specific molecular data are currently available. Here we present, beside an ultrastructural overview, the first proteomic dataset on Pc circulating hemocytes.…”

Section: Doi: 101002/pmic201800314mentioning

confidence: 99%

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Toward the Molecular Deciphering of Pomacea canaliculata Immunity: First Proteomic Analysis of Circulating Hemocytes

et al. 2019

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Pomacea canaliculata is a freshwater snail with interesting biological features that include invasiveness, human parasite hosting, and adult regeneration. Its immune system may represent the target for strategies aimed at controlling the spread of the snail population and its hosting of the human parasite Angiostrongylus cantonensis. Moreover, immune functions likely have a role in the snail's ability to wound heal and regenerate. Despite its importance in multiple processes, very little is known about the molecular basis of P. canaliculata immunity. Aiming to contribute to filling this gap, the ultrastructure of circulating hemocytes in healthy snails is studied and the first proteomic analysis of these cells is performed, evidencing 83 unique proteins, 96% of which have identifiable homologs in other species. Fifteen proteins are retrieved as potentially involved in immune‐related signaling pathways, such as hemocyanin, C1q‐like protein, and HSP90 together with cytoskeleton and cytoskeleton‐related proteins involved in cell motility and membrane dynamics. This first proteome study on non‐stimulated hemocytes provides a valid reference for future investigations on the molecular changes under stressful circumstances, like pathogen exposure, wounding, or environmental changes.

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Section: Proteins Identified From Circulating Hemocytes Of Pc By Lc‐msupporting

confidence: 87%

Section: Doi: 101002/pmic201800314supporting

confidence: 83%

Section: Proteins Identified From Circulating Hemocytes Of Pc By Lc‐mmentioning

confidence: 59%

Section: Proteins Identified From Circulating Hemocytes Of Pc By Lc‐mmentioning

confidence: 99%

Section: Doi: 101002/pmic201800314mentioning

confidence: 99%

See 3 more Smart Citations

Toward the Molecular Deciphering of Pomacea canaliculata Immunity: First Proteomic Analysis of Circulating Hemocytes

et al. 2019

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Mollusca

Dennis

Molnár

Kriska

et al. 2021

Invertebrate Histology

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Novel approach to study gastropod-mediated innate immune reactions against metastrongyloid parasites

et al. 2018

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The anthropozoonotic metastrongyloid nematodes Angiostrongylus cantonensis and Angiostrongylus costaricensis, as well as Angiostrongylus vasorum, Crenosoma vulpis, Aelurostrongylus abstrusus and Troglostrongylus brevior are currently considered as emerging gastropod-borne parasites and have gained growing scientific attention in the last years. However, the knowledge on invertebrate immune responses and on how metastrongyloid larvae are attacked by gastropod immune cells is still limited. This work aims to describe an in vitro system to investigate haemocyte-derived innate immune responses of terrestrial gastropods induced by vital axenic metastrongyloid larvae. We also provide protocols on slug/snail management and breeding under standardized climate conditions (circadian cycle, temperature and humidity) for the generation of parasite-free F0 stages which are essential for immune-related investigations. Adult slug species (Arion lusitanicus, Limax maximus) and giant snails (Achatina fulica) were maintained in fully automated climate chambers until mating and production of fertilized eggs. Newly hatched F0 juvenile specimens were kept under parasite-free conditions before experimental use. An improved protocol for gastropod haemolymph collection and haemocyte isolation was established. Giemsa-stained haemolymph preparations showed adequate haemocyte isolation in all three gastropod species. Additionally, a protocol for the production of axenic first and third stage larvae (L1, L3) was established. Haemocyte functionality was tested in haemocyte-nematode-co-cultures. Scanning electron microscopy (SEM) and light microscopy analyses revealed that gastropod-derived haemocytes formed clusters as well as DNA-rich extracellular aggregates catching larvae and decreasing their motility. These data confirm the usefulness of the presented methods to study haemocyte-mediated gastropod immune responses to better understand the complex biology of gastropod-borne diseases.

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Comparative analysis of circulating hemocytes of the freshwater snail Pomacea canaliculata

Cited by 47 publications

References 29 publications

Toward the Molecular Deciphering of Pomacea canaliculata Immunity: First Proteomic Analysis of Circulating Hemocytes

Toward the Molecular Deciphering of Pomacea canaliculata Immunity: First Proteomic Analysis of Circulating Hemocytes

Mollusca

Novel approach to study gastropod-mediated innate immune reactions against metastrongyloid parasites

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