Comparative Analysis of Clinical and Biological Characteristics of African Swine Fever Virus Isolates from 2013 Year Russian Federation

Власова, Н. Н.; Варенцова, А. А.; Шевченко, И. В.; Zhukov, I. Yu.; Ремыга, С. Г.; Gavrilova, V. L.; Puzankova, O S; Shevtsov, A. A.; Zinyakov, N. G.; Груздев, К. Н.

doi:10.9734/bmrj/2015/12941

Cited by 26 publications

(23 citation statements)

References 12 publications

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“…Specifically, high doses of inoculum (5000 HAD) by intramuscular route resulted lethal for the animals in <10 days with no time for seroconversion, similarly to the experiments performed before by other groups (Blome et al., ). Additional information about this experiment, including virological, clinical and pathological observations was included in a complementary publication (Vlasova et al., ; submitted for publication).…”

Section: Resultsmentioning

confidence: 99%

Detection of African Swine Fever Antibodies in Experimental and Field Samples from the Russian Federation: Implications for Control

Mur

Иголкин

Варенцова

et al. 2014

Transbound Emerg Dis

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African swine fever (ASF) re-entered in Europe in 2007 by Georgia rapidly affecting neighbouring countries. Since then, ASF has caused severe problems to the Russian Federation (RF) and spread to Northern and Western regions, including Ukraine (2012 and 2014) and Belarus (2013). At the beginning of 2014, dead wild boars were found in Lithuania and Poland. Several outbreaks have been later notified in the European Union(EU), affecting domestic pigs and wild boar of Latvia, Lithuania and Poland, and also wild boar in Estonia, causing major problems for the EU pig sector. Some studies have been performed with this ASFV isolate, revealing that it belongs to genotype II and causes an acute form of the disease. However, few data are available about the presence of antibodies in field and experimental samples from the affected area. This study analysed samples from experimental infections with ASFV isolated from the RF in 2013 (74 sera and 3 tissue exudates), and field samples from the RF from 2013 to 2014 (266 samples, including 32 and 7 tissue exudates from domestic pigs and wild boar, respectively). All samples were tested by a commercial ELISA and, some of them (79), also by immunochromatographic tests. Positive and doubtful samples were confirmed by immunoblotting test. Positive results were found in experimental and field samples, which confirm the presence of antibodies against ASFV in the RF. Antibodies were detected in animals inoculated with three different ASFV isolates, with some differences found among them. Only a small percentage of field samples was positive for ASF antibodies (3.7%), in agreement with other observations that reported a high virulence for the ASFV isolates in the area. These results confirm the potential presence of survivor animals that should be considered in affected areas to help design effective control and eradication plans against ASF.

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Section: Resultsmentioning

confidence: 99%

Detection of African Swine Fever Antibodies in Experimental and Field Samples from the Russian Federation: Implications for Control

Mur

Иголкин

Варенцова

et al. 2014

Transbound Emerg Dis

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“…Several experiments have been performed to characterize virulence and clinical appearance of infections by genotype II ASFV isolates circulating in east Europe in wild boar and domestic pigs (Gabriel et al., ; Blome et al., , ; Guinat et al., ; Vlasova et al., ). However, experimental evidence on the clinical aspects of ASF caused by ASFV isolates circulating in the EU (2014) in domestic pigs is not known.…”

Section: Discussionmentioning

confidence: 99%

Experimental Infection of Domestic Pigs with African Swine Fever Virus Lithuania 2014 Genotype II Field Isolate

Gallardo¹,

Soler²,

Nieto³

et al. 2015

Transbound Emerg Dis

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An experimental infection was conducted to evaluate horizontal transmission, clinical, virological and humoral response induced in domestic pigs infected with African swine fever (ASF) genotype II virus circulating in 2014 into the European Union (EU). Ten naive pigs were placed in contact with eight pigs experimentally inoculated with the Lithuanian LT14/1490 ASF virus (ASFV) responsible for the first ASF case detected in wild boar in Lithuania in January 2014. Clinical examination and rectal temperature were recorded each day. Blood sampling from every animal was carried out twice weekly. Blood samples were examined for presence of ASF virus-specific antibodies and for determining the ASFV viral load. From the obtained results, it was concluded that the Lithuanian ASFV induced an acute disease which resulted in 94, 5% mortality. The disease was easily detected by real-time PCR prior to the onset of clinical signs and 33% of the animals seroconverted. All findings were in accordance with observations previously made in domestic pigs and wild boar when infected with ASF genotype II viruses characterized by a high virulence. One in-contact pig remained asymptomatic and survived the infection. The role of such animals in virus transmission would need further investigation.

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“…The second is occurring in the eastern part of Europe and it is caused by strains of ASFV belonging to genotype II that has 100% sequence homology with the ASFV identified in Belarus in June 2013, a genetic variant of the virus introduced in Georgia in 2007 [5]. The latter is a highly virulent strain inducing an acute form of ASF that results in a mortality rate of 94.5–100% in both wild boar and domestic pigs [4, 12–15]. …”

Section: Reviewmentioning

confidence: 99%

Preventive measures aimed at minimizing the risk of African swine fever virus spread in pig farming systems

Rutili²,

2016

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African swine fever (ASF) is one of the most severe diseases of pigs; it has a drastic impact on the pig industry, causing serious socio-economic consequences to pig farmers and pork producers. In Europe, there are currently two main clusters of infection; one in Sardinia caused by strains of African swine fever virus (ASFV) belonging to genotype I and another in Eastern Europe caused by strains of ASFV belonging to genotype II. The latter is inducing an acute form of ASF and it represents a serious threat to the pig sector. ASF is a disease for which there is no effective vaccine; therefore, prevention has a pivotal role in the control strategy of the disease. This review describes the main preventive measures to adopt to mitigate the risk of ASF spread in pig farming systems.

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Comparative Analysis of Clinical and Biological Characteristics of African Swine Fever Virus Isolates from 2013 Year Russian Federation

Cited by 26 publications

References 12 publications

Detection of African Swine Fever Antibodies in Experimental and Field Samples from the Russian Federation: Implications for Control

Detection of African Swine Fever Antibodies in Experimental and Field Samples from the Russian Federation: Implications for Control

Experimental Infection of Domestic Pigs with African Swine Fever Virus Lithuania 2014 Genotype II Field Isolate

Preventive measures aimed at minimizing the risk of African swine fever virus spread in pig farming systems

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