1983
DOI: 10.1128/aac.24.3.450
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Comparative analysis of conjugative plasmids mediating gentamicin resistance in Staphylococcus aureus

Abstract: Five gentamicin-resistant clinical isolates of Staphylococcus aureus were found to contain self-transmissible plassaids of 32 to 37 megadaltons in size. Restriction endonuclease digests of the plasmids were markedly similar to those of reference plasmids of unrelated geographical origin, thus suggesting the significant contribution of common conjugal plasmids to the emergence of gentamicin resistance in S. aureus populations.Recent reports have documented the conjugal transfer of gentamicin resistance (Gm') pl… Show more

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Cited by 73 publications
(54 citation statements)
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“…Phage typing was performed using the international basic set [11] and two sets of experimental phages (for details see [12]. Plasmid-profiles were established by use of the 'boiling method' [13].…”
Section: Methodsmentioning
confidence: 99%
“…Phage typing was performed using the international basic set [11] and two sets of experimental phages (for details see [12]. Plasmid-profiles were established by use of the 'boiling method' [13].…”
Section: Methodsmentioning
confidence: 99%
“…Resistance was initially reported to be plasmid mediated, and resistance plasmids were found in both coagulasenegative and coagulase-positive staphylococci (3,29,33,41). However, while a family of structurally similar plasmids, most of which were conjugative, was found to contain the Gmr genes in staphylococcal isolates from the United States (3,14,19,31), resistance genes in isolates from Europe and Australia were located either on smaller, nonconjugative plasmids or in the chromosome (13,32,35,38). Two transposons (Tn400J and Tn3851) were identified in Australian Staphylococcus aureus isolates that carried Gmr genes (22,38).…”
mentioning
confidence: 99%
“…Conjugative transfer of these plasmids has the following characteristics: transfer occurs at a low frequency (10-5 to progeny per donor input cell); aggregation of mating pairs is not mediated by surface structures or pheromones; mating does not take place in broth but requires a solid substrate (e.g., nitrocellulose or cellulose acetate); plasmid transfer can occur between S. aureus and S. epidermidis (2,11); and genes required for transfer are plasmid encoded (24). Antimicrobial agent resistance genes are transferred either as part of the conjugative replicon (resistance to tobramycin, kanamycin, and gentamicin [Gmr]; ethidium bromide and quaternary ammonium compounds [Qamr]; trimethoprim [Tpr]; and penicillin [Pcr]) (3,14); or are resident on smaller, nonconjugative plasmids that are mobilized by conjugative plasmids (resistance to chloramphenicol [Cmr] and tetracycline [Tcr]) (11). Conjugative staphylococcal plasmids from several geographic areas have considerable restriction fragment similarity, particularly in the putative tra regions (14).…”
mentioning
confidence: 99%
“…Antimicrobial agent resistance genes are transferred either as part of the conjugative replicon (resistance to tobramycin, kanamycin, and gentamicin [Gmr]; ethidium bromide and quaternary ammonium compounds [Qamr]; trimethoprim [Tpr]; and penicillin [Pcr]) (3,14); or are resident on smaller, nonconjugative plasmids that are mobilized by conjugative plasmids (resistance to chloramphenicol [Cmr] and tetracycline [Tcr]) (11). Conjugative staphylococcal plasmids from several geographic areas have considerable restriction fragment similarity, particularly in the putative tra regions (14). Outbreaks of staphylococcal infections caused by Gmr organisms carrying conjugative plasmids have been described by several investigators, and isolates carrying these plasmids seem to have become endemic at some large teaching hospitals (2,19).…”
mentioning
confidence: 99%