Comparative analysis of Cucurbita pepo metabolism throughout fruit development in acorn squash and oilseed pumpkin

Wyatt, Lindsay E.; Strickler, Susan R.; Mueller, Lukas A.; Mazourek, Michael

doi:10.1038/hortres.2016.45

Cited by 26 publications

(22 citation statements)

References 72 publications

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“…Here we observed that the major carotenoid of ripe acorn squash fruits was β-carotene (accumulating up to 23 µg/g FW), while lutein content was low ( Figure S3). These results are consistent with previously described carotenoid levels of acorn squash [21] and of other squash having orange flesh [11]. Two other carotenoid peaks were detected which might be xanthophylls, but further research is required to identify them.…”

Section: Discussionsupporting

confidence: 92%

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Quality and Storability of Trellised Greenhouse-Grown, Winter-Harvested, New Sweet Acorn Squash Hybrids

et al. 2020

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Acorn squash (Cucurbita pepo) is a familiar fruit vegetable in North America, appreciated for its attractive appearance, good flavor, nutritional content and long storage life. A breeding program in Israel has produced three new acorn squash hybrids of enhanced sweetness and flavor. Presently, we evaluated productivity, quality, and storability of these new cultivars in fall plantings. The plants were grown trellised, in an insect-proof greenhouse, for fruit production during the winter to meet consumer demand. The plants were highly productive and bore fruits of superb quality, but there was a high incidence of fungal rots during postharvest cold storage. Pre-treating the fruits with hot water brushing and rinsing before storage was found effective in reducing rot incidence of the fruits stored at 15 °C, but only for one cultivar. Storing the fruits at 10 °C with reduced humidity (Rh 70%) enabled a 3-month shelf life with significantly reduced fruit-rot incidence and minimal effect on fruit quality of all three cultivars. Storage at 20 °C with reduced humidity was suitable for a 1-month period. These protocols for prolonging storage life will help attain controlled, gradual year-round marketing of quality acorn squash at uniform, reasonable price levels for farmers and consumers, and could facilitate overseas export.

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Section: Discussionsupporting

confidence: 92%

“…During fruit development and ripening, sugars, starch and carotenoids accumulate and significantly affect fruit sweetness, texture and nutritional values, respectively [20][21][22]. Fruit acidity in pumpkin and squash is low (0.04-0.3% of FW, pH 6.8) and has no effect on the fruit sensory quality [23,24].…”

Section: Discussionmentioning

confidence: 99%

Quality and Storability of Trellised Greenhouse-Grown, Winter-Harvested, New Sweet Acorn Squash Hybrids

et al. 2020

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“…Brix is used in programs as a proxy for sweetness, and % dry matter is related to starchiness ( Corrigan et al 2001 ; Loy 2004 ). As the starch and sugar pathways are known to be interrelated, it is not suprising that Brix and % dry matter would have high estimated genetic correlations ( Wyatt et al 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Genomic Prediction and Selection for Fruit Traits in Winter Squash

Hernandez¹,

Wyatt²,

Mazourek³

2020

G3 Genes|Genomes|Genetics

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Improving fruit quality is an important but challenging breeding goal in winter squash. Squash breeding in general is resource-intensive, especially in terms of space, and the biology of squash makes it difficult to practice selection on both parents. These restrictions translate to smaller breeding populations and limited use of greenhouse generations, which in turn, limits genetic gain per breeding cycle and increases cycle length. Genomic selection is a promising technology for improving breeding efficiency; yet, few studies have explored its use in horticultural crops. We present results demonstrating the predictive ability of whole-genome models for fruit quality traits. Predictive abilities for quality traits were low to moderate, but sufficient for implementation. To test the use of genomic selection for improving fruit quality, we conducted three rounds of genomic recurrent selection in a butternut squash (Cucurbita moschata) population. Selections were based on a fruit quality index derived from a multi-trait genomic selection model. Remnant seed from selected populations was used to assess realized gain from selection. Analysis revealed significant improvement in fruit quality index value and changes in correlated traits. This study is one of the first empirical studies to evaluate gain from a multi-trait genomic selection model in a resource-limited horticultural crop.

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“…In 2011, the first Cucurbita transcriptome was generated using 454 GS FLX Titanium technology [28]. Subsequently, many differentially expressed genes associated with flowering, disease resistance, fruit size, fruit shape, fruit color, fruit sweetness, fruit nutritional qualities, and chilling sensitivity were determined using RNA-seq [29,31,32,33,34,35,36].…”

Section: Introductionmentioning

confidence: 99%

Morphological, Transcriptomic and Hormonal Characterization of Trimonoecious and Subandroecious Pumpkin (Cucurbita maxima) Suggests Important Roles of Ethylene in Sex Expression

Wang

Yan

Zou

et al. 2019

IJMS

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: Sex expression is a complex process, and in-depth knowledge of its mechanism in pumpkin is important. In this study, young shoot apices at the one-true-leaf stage and 10-leaf stage in Cucurbita maxima trimonoecious line ‘2013–12’ and subandroecious line ‘9–6’ were collected as materials, and transcriptome sequencing was performed using an Illumina HiSeqTM 2000 System. 496 up-regulated genes and 375 down-regulated genes were identified between shoot apices containing mostly male flower buds and only female flower buds. Based on gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, the differentially expressed genes were mainly enriched in the ethylene and auxin synthesis and signal transduction pathways. In addition, shoot apices at the 4-leaf stage were treated with the ethylene-releasing agent 2-chloroethylphosphonic acid (Ethrel), aminoethoxyvinyl glycine (AVG), AgNO3 and indoleacetic acid (IAA). The number of female flowers up to node 20 on the main stem of ‘2013–12’ increased significantly after Ethrel and IAA treatment and decreased significantly after AVG and AgNO3 treatment. The female flowers in ‘9–6’ showed slight changes after treatment with the exogenous chemicals. The expression of key genes in ethylene synthesis and signal transduction (CmaACS7, CmaACO1, CmaETR1 and CmaEIN3) was determined using quantitative RT-PCR, and the expression of these four genes was positively correlated with the number of female flowers in ‘2013–12’. The variations in gene expression, especially that of CmaACS7, after chemical treatment were small in ‘9–6’. From stage 1 (S1) to stage 7 (S7) of flower development, the expression of CmaACS7 in the stamen was much lower than that in the ovary, stigma and style. These transcriptome data and chemical treatment results indicated that IAA might affect pumpkin sex expression by inducing CmaACS7 expression and indirectly affecting ethylene production, and the ethylene synthesis and signal transduction pathways play crucial roles in pumpkin flower sex expression. A possible reason for the differences in sex expression between pumpkin lines ‘2013–12’ and ‘9–6’ was proposed based on the key gene expression. Overall, these transcriptome data and chemical treatment results suggest important roles for ethylene in pumpkin sex expression.

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Comparative analysis of Cucurbita pepo metabolism throughout fruit development in acorn squash and oilseed pumpkin

Cited by 26 publications

References 72 publications

Quality and Storability of Trellised Greenhouse-Grown, Winter-Harvested, New Sweet Acorn Squash Hybrids

Quality and Storability of Trellised Greenhouse-Grown, Winter-Harvested, New Sweet Acorn Squash Hybrids

Genomic Prediction and Selection for Fruit Traits in Winter Squash

Morphological, Transcriptomic and Hormonal Characterization of Trimonoecious and Subandroecious Pumpkin (Cucurbita maxima) Suggests Important Roles of Ethylene in Sex Expression

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