Comparative analysis of cytolethal distending toxin (cdt) genes among Campylobacter jejuni, C. coli and C. fetus strains

Asakura, Masahiro; Samosornsuk, Worada; Taguchi, Masumi; Kobayashi, Kazuhiro; Misawa, Naoaki; Kusumoto, Masahiro; Nishimura, Kazuhiko; Matsuhisa, Akio; Yamasaki, Shinji

doi:10.1016/j.micpath.2007.01.005

Cited by 98 publications

(104 citation statements)

References 29 publications

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“…These results were also 100% correlated with the collective results of API Campy, 16S rRNA gene sequence and detection of hipO gene. The cdt genes were demonstrated to be ubiquitously distributed in C. jejuni, C. coli and C. fetus in a species-specific manner (2,8,26,35) with low interspecies and high intraspecies sequences similarities. Thus, the cdt genes may be a reasonable target for discriminating Campylobacter species.…”

Section: Discussionmentioning

confidence: 99%

“…Stool specimens were collected from healthy poultry at four different places in Thailand including 2 [Skirrow] [Oxoid]) at 42 C under microaerobic condition (10% CO 2 , 5% O 2 and 85% N 2 ) for 48 hr and used for further analysis (Table 1).…”

Section: Methodsmentioning

confidence: 99%

“…The cdt gene clusters in C. jejuni, C. coli and C. fetus were demonstrated to be ubiquitously distributed in each species in a species-specific manner (2,8,26). Recently, we developed a cdt genebased species-specific multiplex PCR assay, which is simple, rapid and reliable method for both detection and identification of C. jejuni, C. coli and C. fetus (unpublished).…”

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confidence: 99%

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Evaluation of a Cytolethal Distending Toxin (cdt) Gene‐Based Species‐Specific Multiplex PCR Assay for the Identification of Campylobacter Strains Isolated from Poultry in Thailand

Samosornsuk

Asakura

Yoshida

et al. 2007

Microbiology and Immunology

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We have recently developed a cytolethal distending toxin (cdt) gene‐based species‐specific multiplex PCR assay for identifying Campylobacter jejuni, C. coli and C. fetus. In the present study, the applicability of this assay was evaluated with 34 Campylobacter‐like organisms isolated from poultry in Thailand for species identification and was compared with other assays including API Campy, 16S rRNA gene sequence, and hippuricase (hipO) gene detection. Of the 34 strains analyzed, 20, 10 and 1 were identified as C. jejuni, C. coli, and Arcobacter cryaerophilus, respectively, and 3 could not be identified by API Campy. However, 16S rRNA gene analysis, showed that all 34 strains are C. jejuni/coli. To discriminate between these 2 species, the hipO gene, which is specifically present in C. jejuni, was examined by PCR and was detected in 20 strains, which were identified as C. jejuni by API Campy but not in the remaining 14 strains. Collective results indicated that 20 strains were C. jejuni whereas the 14 strains were C. coli. When the cdt gene‐based multiplex PCR was employed, however, 19, 20 and 19 strains were identified as C. jejuni while 13, 14 and 13 were identified as C. coli by the cdtA, cdtB and cdtC gene‐based multiplex PCR, respectively. Pulsed‐field gel electrophoresis revealed that C. jejuni and C. coli strains analyzed are genetically diverse. Taken together, these data suggest that the cdt gene‐based multiplex PCR, particularly cdtB gene‐based multiplex PCR, is a simple, rapid and reliable method for identifying the species of Campylobacter strains.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Evaluation of a Cytolethal Distending Toxin (cdt) Gene‐Based Species‐Specific Multiplex PCR Assay for the Identification of Campylobacter Strains Isolated from Poultry in Thailand

Samosornsuk

Asakura

Yoshida

et al. 2007

Microbiology and Immunology

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“…CDT consists of three subunits such as CdtA, CdtB and CdtC [33]. Asakura et al [2] observed that cdt genes were universally distributed in C. jejuni, C. coli and C. fetus in a species-specific manner. Based on this finding, a cdt genebased species-specific multiplex PCR targeting either cdtA, cdtB, or cdtC gene has been developed [3] and evaluated for the accuracy of the species identification with a large number of isolates of C. jejuni, C. coli and C. fetus of clinical or animal origin [14,30].…”

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A Cytolethal Distending Toxin Gene-Based Multiplex PCR Assay for Detection of <i>Campylobacter</i> spp. in Stool Specimens and Comparison with Culture Method

Shiramaru

Asakura

Inoue

et al. 2012

The Journal of Veterinary Medical Science

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ABSTRACT. In this study, we evaluated the applicability of cytolethal distending toxin (cdt) gene-based species-specific multiplex PCR for the direct detection and identification of Campylobacter jejuni, C. coli and C. fetus from stool specimens of patients with gastroenteritis in comparison to culture methods. A total of 711 stool specimens were examined for the isolation or detection of campylobacters by using Skirrow's selective agar culture plates, a filtration method and the multiplex PCR assay. Forty-one and 36 C. jejuni strains were isolated by culture and filtration methods, respectively. In addition, 2 and 3 C. coli strains were isolated by Skirrow and the filtration methods, respectively. However, when the multiplex PCR was employed, the cdtB genes of C. jejuni and C. coli were detected in 45 and 4 stool samples, respectively, and 9 C. jejuni PCR-positive samples by multiplex PCR were negative by culture method. Sequence analysis of the PCR products obtained from 8 stool specimens from which campylobacters were not isolated by culture method but the sequences exactly matched with that of the cdtB gene of C. jejuni strain 81-176. None of the remaining stool samples which were culture negative for campylobacters produced any amplicon. Stool samples were defined as Campylobacterpositive if detected by any method. The sensitivity of the multiplex PCR was 83%, which was higher than Skirrow (74%) and filtration method (66%). These data indicate that cdtB gene-based multiplex PCR is a rapid and more sensitive method to identify the most important species of Campylobacter for human diseases. (248)

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“…Cytolethal distending toxin is thought to be an important virulence factor for this organism. 1 Detection of CLDT is not commercially available and was not attempted from the isolate of this horse. Although this study does not prove that C. fetus subsp.…”

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Isolation ofCampylobacter FetusSubspeciesFetusin a Two-Year-Old Quarterhorse with Chronic Diarrhea of an Undetermined Etiology

2009

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Abstract.A 2-year-old Quarterhorse was evaluated for chronic diarrhea and weight loss of 5 weeks duration after numerous diagnostic tests failed to identify an underlying cause. Historically, the horse was housed at pasture where human household waste vehicles were routinely cleaned and the effluent could run onto the field. Physical examination revealed poor body condition and frequent high-volume diarrhea. Diagnostic testing for Salmonella spp., endoparasites, Cryptosporidium spp., Clostridium spp., and diffuse infiltrative bowel disease were negative. Rectal tissue histopathology failed to identify Mycobacterium spp., spirochetal organisms, or submucosal infiltration with cells. Rectal tissue biopsy and a fresh fecal sample identified numerous Campylobacter organisms with microaerobic culture. Molecular testing revealed the species as Campylobacter fetus subsp. fetus as a possible organism associated with large bowel disease in this filly. The organism was sensitive to fluoroquinolone antimicrobials in vitro. The filly responded transiently to therapy, forming discrete fecal balls after 72 hr of treatment. At 5 months follow-up, the horse had gained weight, was alert and responsive, but reverted back to having soft ''cow-pie'' feces. Reculture of the feces at 9 months failed to identify any Campylobacter organisms. To the authors' knowledge, this is the first report to identify C. fetus subsp. fetus from feces and tissue of a horse with the use of molecular methods. This organism could play a role in the etiology of chronic diarrhea in horses.

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Comparative analysis of cytolethal distending toxin (cdt) genes among Campylobacter jejuni, C. coli and C. fetus strains

Cited by 98 publications

References 29 publications

Evaluation of a Cytolethal Distending Toxin (cdt) Gene‐Based Species‐Specific Multiplex PCR Assay for the Identification of Campylobacter Strains Isolated from Poultry in Thailand

Evaluation of a Cytolethal Distending Toxin (cdt) Gene‐Based Species‐Specific Multiplex PCR Assay for the Identification of Campylobacter Strains Isolated from Poultry in Thailand

A Cytolethal Distending Toxin Gene-Based Multiplex PCR Assay for Detection of <i>Campylobacter</i> spp. in Stool Specimens and Comparison with Culture Method

Isolation ofCampylobacter FetusSubspeciesFetusin a Two-Year-Old Quarterhorse with Chronic Diarrhea of an Undetermined Etiology

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