Comparative analysis of diploid species of Avena L. Using cytogenetic and biochemical markers: Avena pilosa M. B. and A. clauda Dur.

Shelukhina, O. Yu.; Бадаева, Е. Д.; Brezhneva, T. A.; Loskutov, I. G.; Pukhalsky, V. A.

doi:10.1134/s1022795408090111

Cited by 12 publications

(5 citation statements)

References 37 publications

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“…In most genera with linked sites, this condition was present in a subset of species, as in Aristolochia [Berjano et al, 2009], Avena [Shelukhina et al, 2007[Shelukhina et al, , 2008a, Brassica [Hasterok et al, 2006], and Byblis [Fukushima et al, 2011]. The retention of adjacent sites in an extensive taxonomic lineage was observed only in the subfamily Aurantioideae, in which at least one pair of adjacent sites was found in species of 10 out of 11 analyzed genera.…”

Section: Frequency Of Genera With 5s and 45s Rdna On The Same Chromosomementioning

confidence: 97%

Non-Random Distribution of 5S rDNA Sites and Its Association with 45S rDNA in Plant Chromosomes

Roa¹,

Guerra²

2015

Cytogenet Genome Res

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5S and 45S rDNA sites are the best mapped chromosome regions in eukaryotic chromosomes. In this work, a database was built gathering information about the position and number of 5S rDNA sites in 784 plant species, aiming to identify patterns of distribution along the chromosomes and its correlation with the position of 45S rDNA sites. Data revealed that in most karyotypes (54.5%, including polyploids) two 5S rDNA sites (a single pair) are present, with 58.7% of all sites occurring in the short arm, mainly in the proximal region. In karyotypes of angiosperms with only 1 pair of sites (single sites) they are mostly found in the proximal region (52.0%), whereas in karyotypes with multiple sites the location varies according to the average chromosome size. Karyotypes with multiple sites and small chromosomes (<3 µm) often display proximal sites, while medium-sized (between 3 and 6 µm) and large chromosomes (>6 µm) more commonly show terminal or interstitial sites. In species with holokinetic chromosomes, the modal value of sites per karyotype was also 2, but they were found mainly in a terminal position. Adjacent 5S and 45S rDNA sites were often found in the short arm, reflecting the preferential distribution of both sites in this arm. The high frequency of genera with at least 1 species with adjacent 5S and 45S sites reveals that this association appeared several times during angiosperm evolution, but it has been maintained only rarely as the dominant array in plant genera.

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Section: Frequency Of Genera With 5s and 45s Rdna On The Same Chromosomementioning

confidence: 97%

Non-Random Distribution of 5S rDNA Sites and Its Association with 45S rDNA in Plant Chromosomes

Roa¹,

Guerra²

2015

Cytogenet Genome Res

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“…The analysis demonstrated that these oat species were similar in the avenin component composition, although individual accessions differed in the electrophoretic mobilities of certain components. A considerable similarity of A. canariensis and A. longiglumis to the Avena diploid species carrying the As genome variant was demonstrated (Shelukhina et al, 2008). Tetraploid oat species Avena abyssinica, A. vaviloviana, A. barbata, and A. agadiriana were studied using C-banding technique, in situ hybridization with the45S and 5S rDNA probes, and RAPD analysis in comparison with the diploid species carrying different types of the A-genome (A. wiestii, As; A. longiglumis, Al; A. canariensis, Ac; A. damascena, Ad, A. prostrata, Ap).The investigation confirmed that all four tetraploids belong to the same AB-genome group; however A. agadiriana occupies distinct position among others.…”

Section: Investigation Of Phylogenetic Relationshipsmentioning

confidence: 94%

“…These facts confirmed that the C genome had diverged from the ancestral genome before the radiation of the various A genome. Presumably, further evolution of the A-and C-genome species occurred separately (Shelukhina et al, 2008Ahmad et al, 2013. The diploid oat species containing the A genome of two types (Al and Ac) were studied by electrophoresis of grain storage proteins (avenins), chromosome Cbanding, and in situ hybridization with probes pTa71 and pTa794.…”

Section: Investigation Of Phylogenetic Relationshipsmentioning

confidence: 99%

Phylogenetic Relationship of Oats (Avena sativa L): A Guide to Conservation and Utilisation of Genetic Resources

Ahmad¹,

Jehangir²,

Rizvan³

et al. 2020

Int.J.Curr.Microbiol.App.Sci

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“…Accordingly, the hexaploids arose from an original hybridization between a tetraploid species with the genomes A and C, and a diploid species with a D genome. These studies were mainly based on karyotype analyses involving conventional staining [3], C-banding [4][5][6][7][8][9][10][11][12][13] and fluorescent in situ hybridization (FISH) with repetitive DNA probes [14][15][16][17][18][19][20]. However, no definite correspondence among chromosomes from diploid, tetraploid and hexaploid species has yet been confirmed.…”

Section: Introductionmentioning

confidence: 99%

Cytogenetic evidence supports Avena insularis being closely related to hexaploid oats

et al. 2021

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Cytogenetic observations, phylogenetic studies and genome analysis using high-density genetic markers have suggested a tetraploid Avena species carrying the C and D genomes (formerly C and A) to be the donor of all hexaploid oats (AACCDD). However, controversy surrounds which of the three extant CCDD tetraploid species—A. insularis, A. magna and A. murphyi—is most closely related to hexaploid oats. The present work describes a comparative karyotype analysis of these three CCDD tetraploid species and two hexaploid species, A. sativa and A. byzantina. This involved the use of FISH with six simple sequence repeats (SSRs) with the motifs CT, AAC, AAG, ACG, ATC and ACT, two repeated ribosomal sequences, and C genome-specific repetitive DNA. The hybridization pattern of A. insularis with oligonucleotide (AC)10 was also determined and compared with those previously published for A. sativa and A. byzantina. Significant differences in the 5S sites and SSR hybridization patterns of A. murphyi compared to the other CCDD species rule out its being directly involved in the origin of the hexaploids. In contrast, the repetitive and SSR hybridization patterns shown by the D genome chromosomes, and by most of the C genome chromosomes of A. magna and A. insularis, can be equated with the corresponding chromosomes of the hexaploids. Several chromosome hybridization signals seen for A. insularis, but not for A. magna, were shared with the hexaploid oats species, especially with A. byzantina. These diagnostic signals add weight to the idea that the extant A. insularis, or a direct ancestor of it, is the most closely related progenitor of hexaploid oats. The similarity of the chromosome hybridization patterns of the hexaploids and CCDD tetraploids was taken as being indicative of homology. A common chromosome nomenclature for CCDD species based on that of the hexaploid species is proposed.

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Comparative analysis of diploid species of Avena L. Using cytogenetic and biochemical markers: Avena pilosa M. B. and A. clauda Dur.

Cited by 12 publications

References 37 publications

Non-Random Distribution of 5S rDNA Sites and Its Association with 45S rDNA in Plant Chromosomes

Non-Random Distribution of 5S rDNA Sites and Its Association with 45S rDNA in Plant Chromosomes

Phylogenetic Relationship of Oats (Avena sativa L): A Guide to Conservation and Utilisation of Genetic Resources

Cytogenetic evidence supports Avena insularis being closely related to hexaploid oats

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