Given the significance of the relationships between human beings, environment, machines and ergonomics as well as the necessity of using renewable fuels, the present study aimed to investigate the effects of different blends of biodiesel, bioethanol and diesel on noise pollution emitted by a MF285 tractor in stationary and moving modes by the aid of statistical methods. In this respect, the emitted noise was measured using the noise dosimeters and sound level meters in the driver and the bystander's positions, at 1000, 1600 and 2000 RPM in both stationary and moving modes. Then, nine fuel blends of biodiesel, bioethanol and diesel with different volumetric percentages as well as pure diesel were studied. To study the effects of key factors on noise pollution, the factorial experiment was conducted in the form of a completely randomized design, followed by the application of the SPSS Statistics Software Version 19.0. The fuel type nearly affected the noise pollution at the level of 5%, and other factors such as engine rotational speed and the fuel type-engine rotational speed interaction influenced it at the level of 1%. In both the driver and bystander's positions, the minimum and maximum noise pollution occurred at 1000 and 2000 RPM, respectively. The effects of gears along with their twofold and threefold interaction with other factors were not significant. Finally, the results of the present study demonstrated that the B 25 E 4 D 71 fuel, composed of 25% biodiesel and 4% bioethanol, had the lowest noise pollution.