Present study is aimed at assessment of Bholari river sand for its geotechnical characterization and suitability as fine aggregate which is being quarried in Jamshoro district, Sindh, Pakistan. For this purpose, sand samples (n=11) were collected from quarry sites and main river channel. Physical properties reveal that Bholari sand is mainly coarse to fine in size (0.2 mm-5 mm). Average values of fineness modulus, specific gravity, bulk density and void content of collected samples are 2.58, 2.56, 1659.90 kg/m3 and 35.12% respectively which varied within the corresponding permissible ranges of ACI (American Concrete Institute). Carbonate content of about 57.59% is also in agreement with corresponding range for fine aggregate. Petrographic examination revealed that Bholari River sand mainly comprises of quartz and rock fragments with subordinate limestone fragments. As per classification of Dott (1964), Bholari sand is lithic arenite where quartz (50%) occurs as main mineral followed by rock fragments (30%), feldspars (15%) and other opaque minerals (5%). Texturally, the sediments are angular (77%) to sub-round (33%). All these physical properties lie within the range set by National Highway Authority (NHA) and American Standard of Testing Material (ASTM). It is concluded that Bholari River sand is suitable for use in concrete mixed with cement and asphalt.