Comparative analysis of enzymatic stability and amino acid sequences of thermostable alkaline proteases from two haloalkaliphilic bacteria isolated from Coastal region of Gujarat, India

A metagenomic alkaline protease from saline habitat: Cloning, over-expression and functional attributes

“…However, it's apparent that haloalkaliphilic nature of enzyme was maintained. Although, the resistant nature of the enzyme was relatively lower than extracellular alkaline proteases from haloalkaliphilic bacteria and actinomycetes reported earlier [9][10][11][12]18]. The recombinant proteases were relatively more resistant when compared to metagenomic protease.…”

“…Modeling of the 3D structure Three-dimensional structures of the serine protease were modeled using the I-TASSER server for protein 3D structure prediction [18][19][20][21]. The server predicts on the distribution of amino acids, active site moiety, primary and secondary protein structure properties by profile profile alignment (PPA) threading techniques.…”

“…These results are intriguing, as proteases from haloalkaliphilic strains such as, Haloalkaliphilic bacterium O.M.E 1 2 (EU680960) and Oceanobacillus iheyensis O.M.A 1 8 (EU680961) from the same soil, O.M.6.2 were active at elevated temperatures. It was found that enzyme activity increases with increase in temperature to its optimum [18]. …”

Secretory expression of thermostable alkaline protease from <i>Bacillus stearothermophilus</i> FI by using native signal peptide and <b>α</b>-factor secretion signal in <i>Pichia pastoris</i>

“…Among all types of proteases, alkaline protease is the largest subgroup of serine protease enzymes and highly active at extreme alkaline pH. Thermostable alkaline proteases are highly demanded for high temperature processing thus, resulting faster reaction rates, increasing the solubility of substrate and reducing microbial contaminations (Akel et al, 2009;Purohit and Singh, 2011). In this study, thermostable alkaline protease F1 was isolated from Bacillus stearothermophilus.…”

“…It can be isolated from various microbial sources (Bajaj and Sharma, 2011;Fu et al, 2003;Gohel and Singh, 2012;Purohit and Singh, 2011). Among all types of proteases, alkaline protease is the largest subgroup of serine protease enzymes and highly active at extreme alkaline pH.…”