Comparative analysis of genetic diversity among species of Chrysanthemum and its related genera using inter-simple sequence repeat and sequence-related amplified polymorphism markers

Abstract

ABSTRACT. In this study, inter-simple sequence repeats (ISSRs) and sequence-related amplified polymorphism (SRAP) were applied to assess the genetic diversity in 38 species of Chrysanthemum and related genera. A total of 204 and 567 bands were amplified by 24 ISSR and 25 SRAP primers, of which 196 (97%) and 557 (99%) were polymorphic, respectively. The ISSR-based genetic similarity ranged from 0.016 to 0.886 and averaged 0.201, while the SRAP-based genetic similarity varied from 0.010 to 0.811 and averaged 0.122. Both the ISSR and SRAP techniques revealed similar clustering patterns and grouped species of Chrysanthemum and Ajania together. The results of principal coordinate analysis corroborated the unweighted pair group method with arithmetic average clustering. Additionally, results from ISSR and SRAP data were significantly correlated (r = 0.89, P < 0.001). Knowledge about genetic diversity among species can aid the transfer of traits of interest from the wild into cultivated chrysanthemum in future distant interspecific breeding.

Genome-wide identification and expression analysis of SBP-box gene family reveal their involvement in hormone response and abiotic stresses in Chrysanthemum nankingense

“…Chrysanthemum is famous for its ornamental and medicinal value regarded as one of the most valuable floricultural crops in the world ( Zhang et al, 2014 ). Hybridization and artificial selection extensively exist in genus Chrysanthemum , causing that polyploid species and species complexes create highly diversify in ploidy levels, morphology of flowers and leaves, colors of ray florets and environmental tolerances, which bring about great market demand prospects and valuable genetic resources to chrysanthemum breeding ( Ma et al, 2020 ; Qi et al, 2021 ).…”

Genetic Diversity and Relatedness Among Six Ranunculus Species Unraveled by SRAP Markers

“…Therefore, the main aim of this study was to investigate the genetic diversity and phylogenetic relationships in six Ranunculus species using SRAP markers, to establish future breeding and conservation strategies for these plants. SRAP primers proved to be suitable for genetic diversity and phylogenetic analyses among related species and could generate more polymorphic fragments than simple sequence repeats (SSR), inter-simple sequence repeat (ISSR), or RAPD markers (Budak et al, 2004;Talebi et al, 2012;Zhang et al, 2014). Table 1.…”