Comparative analysis of genetic diversity in Indian bitter gourd (Momordica charantia L.) using RAPD and ISSR markers for developing crop improvement strategies

Behera, Tusar Kanti; Singh, Akanksha; Staub, Jack E.

doi:10.1016/j.scienta.2007.08.013

Cited by 72 publications

(48 citation statements)

References 40 publications

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“…Behera et al (2008), who worked with Momordica charantia L. accessions, another Cucurbitacea species, collected in many regions of India, obtained a correlation of 0.70 between data from RAPD and ISSR.…”

Section: Resultsmentioning

confidence: 99%

Agrobiodiversity in Cucurbita spp. landraces collected in Rio de Janeiro assessed by molecular markers

Santos

Rodrigues

Simões

et al. 2012

Crop Breed. Appl. Biotechnol.

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Section: Resultsmentioning

confidence: 99%

Agrobiodiversity in Cucurbita spp. landraces collected in Rio de Janeiro assessed by molecular markers

Santos

Rodrigues

Simões

et al. 2012

Crop Breed. Appl. Biotechnol.

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“…The three groups of T a generated could facilitate routine PCR amplification procedure. To obtain a reliable result from genetic analyses, a high number of primers represent an important advantage (Verma et al, 2007;Behera et al, 2008). Twenty ISSR primers are now available to examine edible seeded C. lanatus genetic variability.…”

Section: Discussionmentioning

confidence: 99%

“…In the present study, the percent of polymorphic bands (97.67%) clearly indicated that the ISSR markers are highly polymorphic and especially informative for estimating genetic relationships. High percentage of polymorphism based on ISSR markers was obtained by Paris et al (2003) in Cucurbita pepo (74%); Behera et al (2008) in Momordica charantia and Verma et al (2007) in Benincasa hispida (>80%). These studies demonstrated that ISSR markers frequently detected a higher level of polymorphism than that detected with other dominant markers (RAPD or AFLP) among Citrullus or other Cucurbitaceae (Paris et al, 2003;Sensoy et al, 2007;Behera et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have been devoted to assess genetic variability of Cucurbitaceae based on molecular markers (Katzir et al, 1996;Levi et al, 2004;Behera et al, 2008). Among a wide range of DNA markers methods available, single sequence repeats (SSR) or microsatellites tend to be the most variable for discriminating among genotypes (Russell et al, 1997;Pejic et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

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Use of ISSR markers to assess genetic diversity of African edible seeded Citrullus lanatus landraces

Djè

Tahi

et al. 2010

Scientia Horticulturae

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“…Результаты и обсуждение. Для сравни-тельного анализа полиморфизма растений, принадлежащих различным систематическим группам, были подобраны ISSR-маркеры, вы-сокополиморфные по результатам предвари-тельных исследований [12,14].…”

Section: таблица 1 характеристика использованных Issr-праймеровunclassified

Selection of Effective Issr-Primers for Genotiping of Cucurbitaceae Plants

Khudyakova¹,

Fedorov²

2018

VESTNIK BSAU

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The article considers a new method of sowing in the early stages of barley breeding. The experimental research was carried out in 2017 in the Krasnoufimsky breeding center. Barley was used as the test culture for seeding. Methods of sowing that have been used were as follows: manual method (method «in the pipe») and the method with the use of the mechanized seeder SSFK-7. In the design of the sowing device SSFK-7, plastic funnels were added, which are located in the apparatus of batch sowing, snug against the blades of the cells. Plastic funnels are located evenly, so that the distance between them is the same, they move in a circle, which ensures the seeding of the seeds in portions, each being a line or a family. The seed tube that goes from the batching machine to the centrifugal seed distributor is disconnected, instead of it the branch pipe is connected to the cross, which distributes the seeds to two extreme right coulters, the other openers in this seeding method are not working. Analysis of the research results showed great practical value of the new method of seeding with the SSFC-7 seeder for the selection of breeding nurseries. The effectiveness of this method is characterized by a reduction in sowing time and labor intensity by 1,9 times. The improvement of the selection process and the replacement of manual labor with mechanized labor will make it possible to significantly increase the amount of selection in the future and to obtain high results in breeding.

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Comparative analysis of genetic diversity in Indian bitter gourd (Momordica charantia L.) using RAPD and ISSR markers for developing crop improvement strategies

Cited by 72 publications

References 40 publications

Agrobiodiversity in Cucurbita spp. landraces collected in Rio de Janeiro assessed by molecular markers

Agrobiodiversity in Cucurbita spp. landraces collected in Rio de Janeiro assessed by molecular markers

Use of ISSR markers to assess genetic diversity of African edible seeded Citrullus lanatus landraces

Selection of Effective Issr-Primers for Genotiping of Cucurbitaceae Plants

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