Comparative analysis of high butanol tolerance and production in clostridia

Patáková, Petra; Kolek, Jan; Sedlář, Karel; Koščová, Pavlína; Branská, Barbora; Kupkova, Kristyna; Paulová, Leona; Provazník, Ivo

doi:10.1016/j.biotechadv.2017.12.004

Cited by 50 publications

(39 citation statements)

References 265 publications

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“…In reality, 15 g/L is the highest relevant concentration for 1-butanol separation from ABE fermentation broth. Certain clostridial strains can produce a maximum concentration of 20-25 g/L of 1-butanol [16]. However, reaching this maximum 1-butanol concentration causes death of the microbial cells.…”

Section: Pervaporation Systemmentioning

confidence: 99%

Transient and Steady Pervaporation of 1-Butanol–Water Mixtures through a Poly[1-(Trimethylsilyl)-1-Propyne] (PTMSP) Membrane

et al. 2019

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The transient and steady pervaporation of 1-butanol–water mixtures through a poly[1-(trimethylsilyl)-1-propyne] (PTMSP) membrane was studied to observe and elucidate the diffusion phenomena in this high-performing organophilic glassy polymer. Pervaporation was studied in a continuous sequence of experiments under conditions appropriate for the separation of bio-butanol from fermentation broths: feed concentrations of 1.5, 3.0 and 4.5 w/w % of 1-butanol in nutrient-containing (yeast extract) water, temperatures of 37, 50 and 63 °C, and a time period of 80 days. In addition, concentration polarization was assessed. As expected, the total flux and individual component permeabilities declined discernibly over the study period, while the separation factor (average β = 82) and selectivity towards 1-butanol (average α = 2.6) remained practically independent of the process conditions tested. Based on measurements of pervaporation transients, for which a new apparatus and model were developed, we found that the diffusivity of 1-butanol in PTMSP decreased over time due to aging and was comparable to that observed using microgravimetry in pure vapor in 1-butanol. Hence, despite the gradual loss of free volume of the aging polymer, the PTMSP membrane showed high and practically independent selectivity towards 1-butanol. Additionally, a new technique for the measurement and evaluation of pervaporation transients using Fourier transform infrared spectroscopy (FTIR) analysis of permeate was proposed and validated.

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Section: Pervaporation Systemmentioning

confidence: 99%

Transient and Steady Pervaporation of 1-Butanol–Water Mixtures through a Poly[1-(Trimethylsilyl)-1-Propyne] (PTMSP) Membrane

et al. 2019

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“…During the long evolutionary process, microorganisms automatically develop a series of strategies to improve cellular robustness against environmental stresses (Fig. 4), including: (1) metabolic detoxification; (2) heat shock stress proteins (HSP); (3) the proton motive force and associated energy production; (4) molecular efflux pumps; (5) changes in cell membrane composition and biophysics, and (6) other transcriptional responses [22,[157][158][159]. At present, several butanol-tolerant strains have been obtained through classical chemical mutagenesis, continuous culture, and serial enrichment procedures [21,160].…”

Section: Improvement Of Cellular Robustness During Abe Fermentationmentioning

confidence: 99%

Pathway dissection, regulation, engineering and application: lessons learned from biobutanol production by solventogenic clostridia

Huang

et al. 2020

Biotechnol Biofuels

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The global energy crisis and limited supply of petroleum fuels have rekindled the interest in utilizing a sustainable biomass to produce biofuel. Butanol, an advanced biofuel, is a superior renewable resource as it has a high energy content and is less hygroscopic than other candidates. At present, the biobutanol route, employing acetone-butanolethanol (ABE) fermentation in Clostridium species, is not economically competitive due to the high cost of feedstocks, low butanol titer, and product inhibition. Based on an analysis of the physiological characteristics of solventogenic clostridia, current advances that enhance ABE fermentation from strain improvement to product separation were systematically reviewed, focusing on: (1) elucidating the metabolic pathway and regulation mechanism of butanol synthesis; (2) enhancing cellular performance and robustness through metabolic engineering, and (3) optimizing the process of ABE fermentation. Finally, perspectives on engineering and exploiting clostridia as cell factories to efficiently produce various chemicals and materials are also discussed.

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“…Their further optimization is often delayed by a lack of efficient genetic tools for complex genetic modification (Wen et al ., ). In addition, the low butanol tolerance of Clostridium determines the upper limit of butanol production (Patakova et al ., ), while inefficient xylose utilization also negatively influences hemicellulose degradation and conversion (Gu et al ., , ). Driven by advancements of clostridial synthetic biotechnology, progress has been made in the improvement of cellulolytic clostridia chassis to optimize CBP.…”

Section: Cbp Optimization By Cellulolytic Clostridia Chassis Engineeringmentioning

confidence: 99%

“…Butanol toxicity is considered a major barrier for butanol accumulation in fermentation media to high titre since this might cause microbial growth inhibition and cell death (Papoutsakis, 2008;Patakova et al, 2018). To date, n-butanol output did not exceed 21 g l À1 , which was achieved by C. beijerinckii BA101in 1999 (Chen and Blaschek, 1999).…”

Section: Improvement Of Butanol Tolerancementioning

confidence: 99%

“…For example, genome editing and metabolic engineering have been used to modify and optimize CBP-enabling strains or consortia (Wen et al, 2017(Wen et al, , 2019. Omics analysis (Patakova et al, 2018;Liu et al, 2019), metabolic and dynamic modelling (Salimi et al, 2010;Liao et al, 2015) and other techniques have been used to analyse butanol tolerance or to optimize the community structure of producers.…”

Section: Introductionmentioning

confidence: 99%

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Consolidated bioprocessing for butanol production of cellulolytic Clostridia: development and optimization

Wen

Liu

et al. 2019

Microbial Biotechnology

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Summary Butanol is an important bulk chemical, as well as a promising renewable gasoline substitute, that is commonly produced by solventogenic Clostridia. The main cost of cellulosic butanol fermentation is caused by cellulases that are required to saccharify lignocellulose, since solventogenic Clostridia cannot efficiently secrete cellulases. However, cellulolytic Clostridia can natively degrade lignocellulose and produce ethanol, acetate, butyrate and even butanol. Therefore, cellulolytic Clostridia offer an alternative to develop consolidated bioprocessing (CBP), which combines cellulase production, lignocellulose hydrolysis and co‐fermentation of hexose/pentose into butanol in one step. This review focuses on CBP advances for butanol production of cellulolytic Clostridia and various synthetic biotechnologies that drive these advances. Moreover, the efforts to optimize the CBP‐enabling cellulolytic Clostridia chassis are also discussed. These include the development of genetic tools, pentose metabolic engineering and the improvement of butanol tolerance. Designer cellulolytic Clostridia or consortium provide a promising approach and resource to accelerate future CBP for butanol production.

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Comparative analysis of high butanol tolerance and production in clostridia

Cited by 50 publications

References 265 publications

Transient and Steady Pervaporation of 1-Butanol–Water Mixtures through a Poly[1-(Trimethylsilyl)-1-Propyne] (PTMSP) Membrane

Transient and Steady Pervaporation of 1-Butanol–Water Mixtures through a Poly[1-(Trimethylsilyl)-1-Propyne] (PTMSP) Membrane

Pathway dissection, regulation, engineering and application: lessons learned from biobutanol production by solventogenic clostridia

Consolidated bioprocessing for butanol production of cellulolytic Clostridia: development and optimization

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