Comparative analysis of in vitro periodontal characteristics of stem cells from apical papilla (SCAP) and periodontal ligament stem cells (PDLSCs)

Chen, Ke; Xiong, Huacui; Huang, Yibin; Liu, Caiqi

doi:10.1016/j.archoralbio.2013.02.010

Cited by 37 publications

(38 citation statements)

References 32 publications

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“…ALP, which was detected during the process of cell mineralization, is considered an early marker of osteo/odontogenic differentiation of stem cells derived from dental tissues (2,3,11,13,26–30). Results of the present study have shown that SCAP/BMP2 had significantly upregulated ALP expressions on the 1st, 8th and 16th day compared with SCAP/Vector, which was similar to that in DPSCs/BMP2 (13).…”

Section: Discussionmentioning

confidence: 99%

Proliferation and odontogenic differentiation of BMP2 gene-transfected stem cells from human tooth apical papilla: An in vitro study

Wen

Zhang

Ling

et al. 2014

International Journal of Molecular Medicine

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Stem cells from the apical papilla (SCAP) have odontogenic potential, which plays a pivotal role in the root dentin development of permanent teeth. Human bone morphogenetic protein 2 (BMP2) is a well-known gene that participates in regulating the odontogenic differentiation of dental tissue-derived stem cells. However, little is known regarding the effects of the BMP2 gene on the proliferation and odontogenic differentiation of SCAP. This study aimed to evaluate the odontogenic differentiation potential of lentiviral-mediated BMP2 gene-transfected human SCAP (SCAP/BMP2) in vitro. SCAP were isolated by enzymatic dissociation of human teeth apical papillae. The multipotential of SCAP was verified by their osteogenic and adipogenic differentiation characteristics. The phenotype of SCAP was evaluated by flow cytometry (FCM). The proliferation status of the blank vector-transfected SCAP (SCAP/Vector) and SCAP/BMP2 was analyzed by a cell counting kit-8 (CCK-8). Odontogenic genes, including alkaline phosphatase (ALP), osteocalcin (OCN), dentin sialophosphoprotein (DSPP) and dentin matrix protein 1 (DMP1) of the two groups of cells were evaluated by quantitative polymerase chain reaction (qPCR). ALP staining and alizarin red (AR) staining of the cells was performed on the 16th day after transfection. In vitro results of CCK-8, qPCR, ALP and AR staining demonstrated that: i) SCAP/BMP2 had a comparable proliferation rate to SCAP/Vector; ii) SCAP/BMP2 presented significantly better potential to differentiate into odontoblasts compared to SCAP/Vector by upregulating ALP, OCN, DSPP and DMP1 genes; iii) more ALP granules and mineralized deposits were formed by SCAP/BMP2 as compared to SCAP/Vector. The results suggested that lentiviral-mediated BMP2 gene transfection enhances the odontogenic differentiation capacity of human SCAP in vitro.

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Section: Discussionmentioning

confidence: 99%

Proliferation and odontogenic differentiation of BMP2 gene-transfected stem cells from human tooth apical papilla: An in vitro study

Wen

Zhang

Ling

et al. 2014

International Journal of Molecular Medicine

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“…Previous reports have demonstrated the ability of SCAP to differentiate into osteoblasts. Furthermore, SCAP have a higher population doubling capacity and produce significantly greater amounts of mineralized matrices compared to periodontal ligament stem cells (PDLSC) [22]. A bioengineered tooth root has been developed which was composed a rootshaped HA/tricalcium phosphate (TCP) scaffold loaded with SCAP and PDLSC [18].…”

Section: Apical Papilla Stem Cellsmentioning

confidence: 99%

“…Dental pulp stem cells (DPSC) were first identified in the dental pulps of human third molars [22]. DPSC have a higher proliferation rate and colony-forming ability than BMMSC [24].…”

Section: Dental Pulp Stem Cellsmentioning

confidence: 99%

Is There a Role for Neural Crest Stem Cells in Periodontal Regeneration?

Tomokiyo

Hynes

Gronthos

et al. 2015

Curr Oral Health Rep

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“…The dental pulp is also a source of adult stem cells. There are several stem cell types in dental tissues, such as stem cells from exfoliated deciduous teeth, dental pulp stem cells, periodontal ligament stem cells, periodontal ligament stem cells from human retained deciduous teeth and stem cells from apical papilla . Previous studies have revealed that dental pulp cells from exfoliated deciduous teeth (De‐DPCs) and apical pulp‐derived cells of permanent teeth (P‐APDCs) with biodegradable scaffolds seeded on the human tooth slices can successfully differentiate into odontoblast‐like cells on the dentin surface .…”

Section: Introductionmentioning

confidence: 99%

Comparative analysis of transcriptomes between apical pulp‐derived cells from deciduous teeth and permanent teeth or dental pulp cells from exfoliated deciduous teeth

Xiong

Chen

2019

The Journal of Gene Medicine

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Background The present study aimed to explore the differences between apical pulp‐derived cells of deciduous teeth (De‐APDCs) and apical pulp‐derived cells of permanent teeth (P‐APDCs)/dental pulp cells from exfoliated deciduous teeth (De‐DPCs). Methods Three types of cells (De‐DPCs, P‐APDCs and De‐APDCs) were cultured by the tissue explant method. Transcriptome sequencing for the three types of cells was conducted and the differentially expressed genes (DEGs) between groups were selected. The potential biological functions and pathways enriched by the DEGs were analyzed. Results There were 35 up‐ and 21 down‐regulated DEGs in P‐APDC versus the De‐APDC comparison group, and 328 up‐ and 976 down‐regulated DEGs in De‐APDC versus De‐DPC. The DEGs in the P‐APDC versus De‐APDC group were significantly enriched in biological process terms associated with extracellular matrix organization, and regulation of cell proliferation and the pathway of cell adhesion molecules. Additionally, the DEGs between De‐APDC and De‐DPC were involved in functions related to extracellular matrix organization and the regulation of bone remodeling, as well as the pathway of extracellular matrix‐receptor interaction. Conclusions There were fewer differences between P‐APDC and De‐APDC compared to between De‐APDC and De‐DPC in view of the total number of DEGs. Differences in function existed relating to extracellular matrix organization among the three cells. P‐APDCs and De‐APDC had different functions on cell proliferation and adhesion. De‐APDCs may also act as a unique stem cell resource for tissue engineering.

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Comparative analysis of in vitro periodontal characteristics of stem cells from apical papilla (SCAP) and periodontal ligament stem cells (PDLSCs)

Cited by 37 publications

References 32 publications

Proliferation and odontogenic differentiation of BMP2 gene-transfected stem cells from human tooth apical papilla: An in vitro study

Proliferation and odontogenic differentiation of BMP2 gene-transfected stem cells from human tooth apical papilla: An in vitro study

Is There a Role for Neural Crest Stem Cells in Periodontal Regeneration?

Comparative analysis of transcriptomes between apical pulp‐derived cells from deciduous teeth and permanent teeth or dental pulp cells from exfoliated deciduous teeth

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