Comparative analysis of in vitro characteristics of fresh and frozen-thawed urethral and epididymal spermatozoa from cats (Felis domesticus)

Prochowska, Sylwia; Niżański, Wojciech; Partyka, Agnieszka

doi:10.1016/j.theriogenology.2016.07.002

Cited by 34 publications

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“…Because no significant differences were found, we can state that spermatozoa from both types of semen equally retain their ability to produce embryos after freezing-thawing. The present study completes our previous reports about comparable characteristics of spermatozoa collected from the urethra and epididymis (Prochowska et al 2015, Prochowska et al 2016 and finishes an overall picture of the quality of urethral semen.…”

Section: Discussionsupporting

confidence: 89%

“…On the contrary, in swine cryopreserved epididymal semen appeared to be superior to ejaculated, both fresh and frozen-thawed, and the authors linked it with high post-thaw motility of epididymal spermatozoa (Rath and Niemman 1997). For cats, we did not observe differences in the post thawed motility, viability, acrosome integrity or mitochondrial function between urethral and epididymal sperm cells (Prochowska et al 2016), which may explain lack of difference in fertilizing potential. This study seems to prove that both types of spermatozoa comparably maintain anatomical and functional features that are necessary to penetrate and fertilize an oocyte.…”

Section: Discussioncontrasting

confidence: 53%

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In vitro fertilizing potential of urethral and epididymal spermatozoa collected from domestic cats (Felis catus)

Prochowska¹,

Niżański²

2017

Polish Journal of Veterinary Sciences

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The aim of this study was to provide a comparative analysis of in vitro fertilizing potential of frozen-thawed urethral and epididymal feline spermatozoa. Both types of semen were collected from 7 cats and cryopreserved in liquid nitrogen. To perform in vitro fertilization, both urethral and epididymal samples from the same individual were thawed and spermatozoa were co-incubated with in vitro matured cat oocytes. Obtained embryos were cultured in vitro for 7 days in a commercial medium. Cleavage rate, morula rate and blastocyst rate were calculated. Experiment was run in 10 replicates. The examined parameters showed no significant differences between urethral and epididymal spermatozoa (p>0.05). Cleavage rate and embryo's development were highly variable between replicates, even for the different sperm samples collected from one individual. There was no significant correlation between fertilizing capacity of two types of spermatozoa collected from the same male. In this study we confirmed that cryopreserved urethral spermatozoa have equally good fertilizing potential as epididymal ones, and both can be successfully used for in vitro fertilization in cats with the use of commercial medium.

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Section: Discussionsupporting

confidence: 89%

Section: Discussioncontrasting

confidence: 53%

In vitro fertilizing potential of urethral and epididymal spermatozoa collected from domestic cats (Felis catus)

Prochowska¹,

Niżański²

2017

Polish Journal of Veterinary Sciences

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“…Flow cytometric approach allowed for identification of different patterns of changes during different methods of semen preservation. Cryopreservation caused mostly loss of membrane and acrosome integrity, which is a common finding in many species (Niżański, Klimowicz, Partyka, Savić, & Dubiel, ; Partyka, Nizański, & Łukaszewicz, ; Prochowska, Niżański, & Partyka, ) and was reported previously for chinchillas as well (Ponce et al., ). This can be explained by the strong damage that occurs during freezing and thawing (Ahmad, Nasrullah, Riaz, Sattar, & Ahmad, ; Yoon, Kwon, Rahman, Lee, & Pang, ), but also by the addition of glycerol, which also resulted in membrane and acrosome damages (Yoon et al., ).…”

Section: Discussionsupporting

confidence: 70%

Comparison of the characteristics of chinchilla epidydimal semen after collection, storage at 5°C and cryopreservation

Polit

Prochowska

Niżański

2018

Reprod Domestic Animals

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Contents The aim of the study was to compare the characteristics of chinchilla epididymal sperm: fresh, stored at liquid state and cryopreserved. Epididymal spermatozoa obtained from 11 males were assessed for subjective motility, concentration, motility parameters measured by CASA, viability, morphology, membrane integrity, acrosome integrity, mitochondrial potential, lipid peroxidation, chromatin structure, apoptotic changes and capacitation. Then half of the spermatozoa were stored at 5°C for 30 hr, and the second half was cryopreserved. After storage and thawing the same parameters as in fresh semen were assessed. Fresh semen showed good quality, with low levels of lipid peroxidation, chromatin fragmentation and capacitation. CASA evaluation showed significantly lower values for MOT, PMOT, RAPID, VCL, VAP and VSL after both storage at liquid state and cryopreservation (p < 0.05). Cold storage did not induce membrane and acrosome damage (p > 0.05), conversely to cryopreservation (p < 0.05). After storage, there was a drop in high mitochondrial potential in live cells (p < 0.05) and an increase in the percentage of non‐apoptotic, capacitated cells (p < 0.05). These changes were not seen after cryopreservation (p > 0.05). Lipid peroxidation in live cells and chromatin structure remained unchanged both after storage and cryopreservation (p > 0.05). The study showed that examined methods of semen preservation exerted different patterns of changes in spermatozoa and that sperm quality after both of them allowed for further use of preserved spermatozoa in artificial reproductive techniques.

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“…При применении SF более 40 % сперматозоидов в среднем сохраняют жизнеспособность, причем криоконсервация не отражается на доле морфо-логически нормальных сперматозоидов. По данным ли-тературы, она варьирует для эпидидимального семени у разных видов кошачьих от 25.0 до 78.5 % (Prochowska et al, 2016), и приведенные здесь результаты укладываются в этот интервал. Следует отметить, что наиболее частыми аномалиями сперматозоидов в нашем исследовании были комбинированные и аномалии хвоста, что согласуется с данными других исследований (Contri et al, 2012).…”

Section: Discussionunclassified

“…Обычно процедуры замораживания -оттаивания снижают жизнеспособность сперматозоидов эпидидимального семени домашних котов на 20-30 % (Luvoni, 2006;Kunkitti et al, 2016;Prochowska et al, 2016).…”

Section: Discussionunclassified

Cryopreservation of epididymal semen of domestic cat

Amstislavsky¹,

Brusentsev²,

Mokrousova³

et al. 2017

Vestn. VOGiS

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Domestic cat (Felis silvestris catus)is used as a model species for developing effective methods of wild felids' semen cryopreservation. The present study represents a comparison of domestic cat semen cryopreservation with two commercially available cryoprotectant agents (CPAs): CaniPlus Freeze (CPF) and SpermFreeze (SF). Semen was collected from the caudal epididymises of adult males and frozen with CPF and SF, correspondingly. The viability of frozen-thawed spermatozoa was evaluated by VitalScreen kit, staining with hematoxylin and eosin was performed for analysis of the spermatozoa morphology; both analyses were combined with the light microscopy. The viability rate of the frozenthawed semen cryopreserved with CPF and SF did not differ: 32.3 ± 4.4 % for CPF and 43.3 ± 4.0 % for SF. Total percentage of morphologically normal spermatozoa after freezing and thawing domestic cat semen was 26.0 ± 2.3 % for CPF and 23.9 ± 1.9 % for SF. In both cases, there were no differences from non-frozen semen, in the latter group the total percentage of spermatozoa with normal morphology was 29.0 ± 4.1 %. The most frequent anomalies were the anomalies of tail, and the rarest anomalies were head defects. The percentages of spermatozoa with anomalies of the head, mid piece, tail and combined did not differ in these three groups. Taken together these results suggest that both CPAs are suitable for the purpose of domestic cat semen freezing and cryopreservation, although CPF was designed for Canidae semen cryopreservation and SF was developed for human semen freezing and so far was used exclusively in reproductive medicine. It might be concluded that these two commercially available cryoprotectant media are applicable for the purposes of domestic cat breeds' semen cryopreservation.Key words: domestic cat; epididymal spermatozoa; cryopreservation.Домашний кот (Felis silvestris catus) используется в качестве мо-дельного объекта для разработки эффективных методов крио-консервирования семени исчезающих видов кошачьих. В иссле-довании представлено сравнение двух коммерчески доступных криопротекторных смесей CaniPlus Freeze (CPF) и SpermFreeze (SF), используемых для криоконсервации семени домашнего кота (Felis silvestris catus). Семя получали из каудальных эпидидимисов взрос-лых самцов и замораживали с помощью CPF либо SF. Жизнеспо-собность замороженно-оттаянных сперматозоидов оценивали методом VitalScreen; морфологический анализ семени проводили с использованием окраски гематоксилином и эозином; в обоих случаях окраску сочетали со световой микроскопией. Доля живых сперматозоидов после замораживания с применением CPF и SF достоверно не различалась: 32.3 ± 4.4 % для CPF и 43.3 ± 4.0 % для SF. Доля морфологически нормальных сперматозоидов после процедур замораживания-оттаивания семени домашнего кота составила 26.0 ± 2.3 % для CPF и 23.9 ± 1.9 % для SF. В обоих случаях не было достоверных отличий по этому показателю от интактного (не подвергавшегося замораживанию) семени, в этой группе доля сперматозоидов с нормальной морфологией составила...

show abstract

Comparative analysis of in vitro characteristics of fresh and frozen-thawed urethral and epididymal spermatozoa from cats (Felis domesticus)

Cited by 34 publications

References 40 publications

In vitro fertilizing potential of urethral and epididymal spermatozoa collected from domestic cats (Felis catus)

In vitro fertilizing potential of urethral and epididymal spermatozoa collected from domestic cats (Felis catus)

Comparison of the characteristics of chinchilla epidydimal semen after collection, storage at 5°C and cryopreservation

Cryopreservation of epididymal semen of domestic cat

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