Comparative analysis of intraluteal steroidogenic enzymes emphasises the functionality of fresh and persistent corpora lutea during pro—and metoestrus in the lynx

Zschockelt, Lina; Amelkina, Olga; Koster, Stefanie; Painer, Johanna; Okuyama, Minami W.; Serra, Rodrigo; Vargas, Astrid; Jewgenow, Katarina; Braun, Beate C.

doi:10.1016/j.jsbmb.2015.07.001

Cited by 12 publications

(7 citation statements)

References 45 publications

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“…According to Hanukoglu et al 15 , the relevant P450 enzyme for producing superoxide anion in luteal cells is CYP11A1 (Cholesterol side chain cleavage enzyme). We demonstrated that the gene expression of CYP11A1 was significantly elevated in persistent CL samples of Iberian lynx compared to the fresh CL, although the expression in Eurasian lynx samples was less pronounced 12 . In contrast, immunohistochemistry for CYP11A1 did not show stronger signals in persistent CL of both lynx species compared to fresh Iberian lynx CL samples 12 .…”

Section: Discussionmentioning

confidence: 78%

“…Collection of Iberian lynx (Lynx pardinus) CL samples in Spain (two animals, IL1 and IL2, 7 days after mating) and of Eurasian lynx (Lynx lynx) samples (CL of 5 animals, before mating season) in Norway (ELG = Eurasian lynx group) is described in Zschockelt et al 12 .…”

Section: Methodsmentioning

confidence: 99%

“…Quantitative real-time pcR. Homogenization, followed by total RNA extraction and reverse transcription to cDNA for ELG and IL samples was already described in previous studies 5,12,44 . Samples of EL1 were handled identically.…”

Section: Scientific Reports |mentioning

confidence: 99%

“…Glyceraldehyde 3-phosphate dehydrogenase (GAPDH), Glutaminase (GLS), TATA box binding protein (TBP), β-actin (BACT), and ribosomal protein S7 (RPS7) were tested as reference genes (for qPCR conditions see 12,56,58 ). Based on analysis with qbasePLUS software (Biogazelle, Zwijnaarde, Belgium 59 ), GAPDH, GLS, TBP and BACT were chosen as optimal reference genes and were used for normalization.…”

Section: Scientific Reports |mentioning

confidence: 99%

“…A very particular exception are lynxes, where persistent CL can be found 6-9 that are not pathological as observed for other species like cows 10 . These persistent CL remain functionally active with continuous production of progesterone [11][12][13] . Possibly, this is part of a mechanism controlling the monooestrus in three of four lynx species 6 and/or may be supporting early pregnancies of following cycles by their additional hormone production 14 .…”

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confidence: 99%

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The antioxidative enzyme SOD2 is important for physiological persistence of corpora lutea in lynxes

Braun

Halaski

Painer

et al. 2020

Sci Rep

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Corpora lutea (cL) are transient endocrine glands supporting pregnancy by progesterone production. they develop at the site of ovulation from the remaining follicle, are highly metabolically active and undergo distinct, transformative processes during their lifetime. in contrast to other species, cL of lynxes do not regress at the end of cycle, but remain functionally active (persist) for years. Reactive oxygen species (RoS) and anti-oxidative enzymes are described to be important for the functionality of cL. We examined ten anti-oxidative enzymes in fresh and persistent cL of lynxes as well as in domestic cat CL of different luteal stages. The gene expression profiles, especially those of SOD1 and SOD2, showed some remarkable differences between CL stages during non-pregnant and pregnant cycles of domestic cats and between fresh and persistent CL of lynxes. Lynx gene expression profiles of SODs were confirmed by western blot analysis, immunohistochemistry and activity assays. SOD2 was characterized by a conspicuous high expression and enzyme activity exclusively in persistent cL. We suggest that SOD2 is required to detoxify potential elevated superoxide anion levels by producing H 2 o 2 in the physiologically persistent cL. this product might also act as a signaling molecule, securing the cL from apoptosis and insuring long-term luteal cell survival.Corpora lutea (CL) are transient ovarian endocrine glands that are formed at the site of ovulation through transition of cells from the remaining disrupted follicle. By producing progesterone they support pregnancies 1 . These glands are highly metabolically active and undergo distinct, transformative processes 2 during their lifetime of days (e.g. in rats), weeks (e.g. in dogs) 3 or months (elephants) 4 . They pass different stages over formation, development/maintenance and regression; ending in the corpus albicans stage 2 , also described for domestic cat 5 . The latter stage usually confirms the end of a luteal phase. A very particular exception are lynxes, where persistent CL can be found 6-9 that are not pathological as observed for other species like cows 10 . These persistent CL remain functionally active with continuous production of progesterone [11][12][13] . Possibly, this is part of a mechanism controlling the monooestrus in three of four lynx species 6 and/or may be supporting early pregnancies of following cycles by their additional hormone production 14 . The persistence of these CL is confirmed to be several years 6 .Reactive oxygen species (ROS) are a byproduct of steroid hormone production 15 and anti-oxidative enzymes seem to protect the CL against luteolysis and apoptosis during the maintenance stage 16,17 . Furthermore it is described that ROS are elevated during luteal regression and that anti-oxidative enzymes can rescue CL from regression during pregnancy, see for review 16 . In cells, ROS like superoxide radical or hydrogen peroxide are a result of diverse activities. ROS are part in physiologically positive processes like signaling 18 but t...

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Section: Discussionmentioning

confidence: 78%

Section: Methodsmentioning

confidence: 99%

Section: Scientific Reports |mentioning

confidence: 99%

Section: Scientific Reports |mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

The antioxidative enzyme SOD2 is important for physiological persistence of corpora lutea in lynxes

Braun

Halaski

Painer

et al. 2020

Sci Rep

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Regulation of Corpus Luteum Function in the Domestic Dog (Canis familiaris) and Comparative Aspects of Luteal Function in the Domestic Cat (Felis catus)

Kowalewski

2016

The Life Cycle of the Corpus Luteum

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Development of a 3D in vitro model to study corpus luteum of felids based on luteinized cells from antral follicles

Hryciuk,

Schröter,

Claaßen

et al. 2024

Cell Tissue Res

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The study aimed to establish a long-term 3D cell culture model using luteinized follicular cells to investigate the functionality and life cycle of the CL in felids. A mixture of cell types from antral follicles was luteinized in vitro and cultured for up to 23 days. The method, initially applied to the domestic cat, was later extended to Persian and Clouded leopards. Antral follicles were isolated and digested with enzymes; then, the cells were subjected to culture. Experimental subsets were treated with/without 1 µg/mL cloprostenol to validate the cell culture model’s suitability for functional studies. In domestic cat samples, microscopic evaluation indicated luteinization, which was confirmed by increased progestagen concentrations and IHC staining for HSD3B and CYP11A1. The gene expression of selected steroidogenic factors (HSD3B1, STAR, CYP11A1) and hormone receptors (LHCGR, PTGFR, PRLR) significantly increased, while CYP17A1 expression decreased. Cloprostenol treatment resulted in reduction of steroidogenic activity, proving its suitability for functional studies. Persian and Clouded leopards’ cell cultures exhibited similar patterns in progestagen secretion and gene expression, compared to domestic cats. This model, with its defined luteinization, as well as high and stable progestagen production, allows future investigation of factors regulating CL life cycle and function.

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Comparative analysis of intraluteal steroidogenic enzymes emphasises the functionality of fresh and persistent corpora lutea during pro—and metoestrus in the lynx

Cited by 12 publications

References 45 publications

The antioxidative enzyme SOD2 is important for physiological persistence of corpora lutea in lynxes

The antioxidative enzyme SOD2 is important for physiological persistence of corpora lutea in lynxes

Regulation of Corpus Luteum Function in the Domestic Dog (Canis familiaris) and Comparative Aspects of Luteal Function in the Domestic Cat (Felis catus)

Development of a 3D in vitro model to study corpus luteum of felids based on luteinized cells from antral follicles

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