Comparative analysis of iol clusters in Lactobacillus casei strains

Abstract

The present study was designed to expand genetic knowledge of myo-inositol (MI) metabolism in Lactobacillus casei. Twenty-four L. casei isolates of dairy origin were tested for the presence of iol cluster. PCR screening revealed eight strains encoded functions involved in MI utilization, of which one strain was able to use MI as carbon source. To gain a deeper understanding of the function of iol genes, four of the eight observed iol clusters were subjected to the full sequencing procedure. The results showed that the iol cluster was not a common feature among dairy L. casei strains. In addition, the four iol clusters were highly similar to one another in terms of sequence similarity and operon architecture. However, abundant polymorphisms that comprised a majority of synonymous mutations were detected throughout the full sequences. Three of them distributed among iolB, iolC, and iolT genes were found in linkage to MI-negative phenotype. Compared with other bacterial iol clusters, the iol cluster of L. casei showed a high similarity with that of Bacillus subtilis.

Comparative genomics and functional analysis of a highly adhesive dairy Lactobacillus paracasei subsp. paracasei IBB3423 strain

“…So far, only some strains of L. casei have been found to metabolize MI with poor efficiency. One should note that even the presence of genes encoding enzymes for MI metabolism may not be sufficient to confer an ability to utilize MI (Zhang et al 2010; Vinay-Lara et al 2014). Moreover, we identified glgBCDAP-amyB g enes connected with glycogen metabolism ( LCAKO_2211 to LCAKO_2216 ) organized identically to operons of other Lactobacillus species (Goh and Klaenhammer 2014).…”

Physiological, Biochemical, and Structural Bioinformatic Analysis of the Multiple Inositol Dehydrogenases from Corynebacterium glutamicum

“…In many inositol-degrading bacteria, multiple paralogous genes annotated or shown to encode IDHs were identified ( 39 – 41 ), which, in the case of B. subtilis , for example, enable growth not only on MI but also on SI and DCI ( 42 , 43 ). In C. glutamicum , seven potential inositol oxidoreductases were annotated, three in cluster iol1 (IolG, IolW, and OxiB), three in cluster iol2 (OxiC, OxiD, and OxiE), and another one elsewhere in the genome (IdhA3) ( Fig.…”

Comparative genomic and metabolic analysis of three Lactobacillus paracasei cheese isolates reveals considerable genomic differences in strains from the same niche

“…It can also be metabolised by bacteria that live in soil, but it is not frequently used as an energy source in LAB [ 39 ]. So far, strains of L. casei are the only members of LAB that are capable of weakly metabolizing MI, but the presence of a MI metabolism cluster of genes is not a common feature of L. casei strains, and it does not necessarily mean that the strain carrying the cluster will use it as an energy source [ 40 ]. Previously, the presence of the complete MI utilisation operon was confirmed in the probiotic strain L. casei BL23 [ 39 ].…”