Comparative analysis of methods for collection and measurement of cytokines and immunoglobulins in cervical and vaginal secretions of HIV and HPV infected women

Snowhite, Isaac; Jones, William; Dumestre, Jeanne; Dunlap, Kathleen; Braly, Patricia S.; Hagensee, Michael E.

doi:10.1016/s0022-1759(02)00038-8

Cited by 31 publications

(33 citation statements)

References 18 publications

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“…Secretion of IL-13 was reported within the female genital tract [35], whereas a decrease in secretion of IL-13 by PBMC was reported along the progressive HIV infection [36]. A review of genomic data also found that infection of macrophages and CD4 ϩ T cells by HIV-1 resulted in the downmodulation of IL-13 mRNA transcription [37].…”

Section: Discussionmentioning

confidence: 99%

Differential modulation of CCR5-tropic human immunodeficiency virus–1 transfer from macrophages towards T cells under interleukin-4/interleukin-13 microenvironment

Saïdi¹,

Carbonneil²,

Magri³

et al. 2010

Human Immunology

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Section: Discussionmentioning

confidence: 99%

Differential modulation of CCR5-tropic human immunodeficiency virus–1 transfer from macrophages towards T cells under interleukin-4/interleukin-13 microenvironment

Saïdi¹,

Carbonneil²,

Magri³

et al. 2010

Human Immunology

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“…Alternative techniques for recovering genital tract secretions, such as wicking methods (Sno-Strip [Akorn] or TearFlo [Wilson Ophthalmic]), may have provided less dilute samples. However, wicking methods may induce an inflammatory response, and, in addition, a recent study suggested that lavage yielded more reproducible cytokine levels than did wicking methods [20]. In the lavage procedure used, the applied 2-mL dose of gel and resident vaginal secretions are diluted with 10 mL of saline.…”

Section: Discussionmentioning

confidence: 99%

PRO 2000 Gel Inhibits HIV and Herpes Simplex Virus Infection Following Vaginal Application: A Double‐Blind Placebo‐Controlled Trial

Keller¹,

Zerhouni-Layachi²,

Cheshenko

et al. 2006

J INFECT DIS

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“…However, unlike cervicovaginal lavages that represent an admixture of secretions of the cervix and vagina, possibly two immunologically distinct anatomical sites (6,14), this method, like Sno-Strips wicks (Akron, Abita Springs, La.) (19), collects undiluted cervical secretions. Previously, we have shown that the collection of cervical secretions by using a Weck-Cel (XOMED Surgical Products, Inc., Jacksonville, Fla.) ophthalmic sponge was a minimally invasive and broadly useful method of specimen collection that did not negatively impact the performance of Pap smears for the detection of cytologic abnormalities (11).…”

mentioning

confidence: 99%

Comparison of Ophthalmic Sponges for Measurements of Immune Markers from Cervical Secretions

Castle

Rodríguez

Bowman

et al. 2004

Clin Vaccine Immunol

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Measurements of cervical immunity are important for evaluating immune responses to infections of the cervix and to vaccines for preventing those infections. Three ophthalmic sponges, Weck-Cel, Ultracell, and Merocel, were loaded in vitro with interleukin-1␤ (IL-1␤), IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12, IL-15, IL-18, gamma interferon (IFN-␥), granulocyte-macrophage colony-stimulating factor (GM-CSF), immunoglobulin A (IgA), or IgG, and sponges were extracted and evaluated for total recovery by enzyme-linked immunosorbent assay (ELISA). There was excellent (>75%) recovery for all immune markers from all three devices except for IL-6, which was poorly recovered (<60%) for all sponge types, IFN-␥, which was poorly recovered from both Weck-Cel and Ultracell sponges but was completely recovered from Merocel sponges, and IL-4, which was poorly recovered from Weck-Cel sponges but was completely recovered from Ultracell or Merocel sponges. We then compared the absolute recovery of selected markers (IL-10, IL-12, IgG, and IgA) from cervical secretion specimens collected from women using each type of sponge. There were no significant differences in the recoveries of IL-10, IL-12, and IgG from cervical specimens collected by any type of ophthalmic sponge, but there was reduced IgA recovery from Merocel sponges. However, the variability in these measurements attributable to sponge types (1 to 3%) was much less than was attributable to individuals (45 to 72%), suggesting that differences in sponge type contribute only in a minor way to these measurements. We infer from our data that the three collection devices are adequate for the measurements of IL-1␤, IL-2, IL-5, IL-12, IL-15, IL-18, and IgG. Merocel may be a better ophthalmic sponge for the collection of cervical secretions and measurements of IL-4, IL-8, IL-10, GM-CSF, and IFN-␥, but our data from clinical specimens, not in vitro-loaded sponges, suggested the possibility of reduced recovery of IgA. These findings require confirmation.We have been interested in optimizing the collection of cervical secretions by using ophthalmic sponges to evaluate cervical immunity in the context of host response to and vaccination against human papillomavirus (HPV) infection (3-5, 7, 8, 11-13, 15). The measurement of immunity at the cervix may be necessary to understand immune responses to HPV, given the localized nature of infection and the poor correlation between levels of immunity in blood and cervical secretions (3, 4). There are numerous methods for sampling genital tract secretions, including such methods as cervicovaginal lavage and passive absorption. However, unlike cervicovaginal lavages that represent an admixture of secretions of the cervix and vagina, possibly two immunologically distinct anatomical sites (6,14), this method, like Sno-Strips wicks (Akron, Abita Springs, La.) (19), collects undiluted cervical secretions. Previously, we have shown that the collection of cervical secretions by using a Weck-Cel (XOMED Surgical Products, Inc., Jacksonville, Fla.) ophtha...

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Comparative analysis of methods for collection and measurement of cytokines and immunoglobulins in cervical and vaginal secretions of HIV and HPV infected women

Cited by 31 publications

References 18 publications

Differential modulation of CCR5-tropic human immunodeficiency virus–1 transfer from macrophages towards T cells under interleukin-4/interleukin-13 microenvironment

Differential modulation of CCR5-tropic human immunodeficiency virus–1 transfer from macrophages towards T cells under interleukin-4/interleukin-13 microenvironment

PRO 2000 Gel Inhibits HIV and Herpes Simplex Virus Infection Following Vaginal Application: A Double‐Blind Placebo‐Controlled Trial

Comparison of Ophthalmic Sponges for Measurements of Immune Markers from Cervical Secretions

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