Comparative analysis of natural and synthetic antimutagens as regulators of gene expression in human cells under exposure to ionizing radiation

Михайлов, В. Ф.; Шишкіна, А. А.; Vasilyeva, I.G.; Shulenina, L. V.; Раева, Н. Ф.; Рогожин, Е. А.; Startsev, M. I.; Zasukhina, G. D.; Gromov, S. P.; Алфимов, М. В.

doi:10.1134/s102279541411009x

Cited by 4 publications

(3 citation statements)

References 24 publications

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“…Their influence on the expression level of oncogenes, tumor suppressor genes [ 58 , 59 , 60 ], and ability to bind DNA can also decrease the toxic effect of heavy metal ions [ 61 ]. These properties are also found in thionins from wild dicots [ 62 , 63 , 64 , 65 , 66 ]. These molecules belong to the eight-cysteine thionin subfamily.…”

Section: Investigation Of Structural Determinants Of Other Amps (Tmentioning

confidence: 89%

Primary Structure Analysis of Antifungal Peptides from Cultivated and Wild Cereals

Рогожин

Ryazantsev

Smirnov

et al. 2018

Plants

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Cereal-derived bioactive peptides with antimicrobial activity have been poorly explored compared to those from dicotyledonous plants. Furthermore, there are a few reports addressing the structural differences between antimicrobial peptides (AMPs) from cultivated and wild cereals, which may shed light on significant varieties in the range and level of their antimicrobial activity. We performed a primary structure analysis of some antimicrobial peptides from wild and cultivated cereals to find out the features that are associated with the much higher antimicrobial resistance characteristic of wild plants. In this review, we identified and analyzed the main parameters determining significant antifungal activity. They relate to a high variability level in the sequences of C-terminal fragments and a high content of hydrophobic amino acid residues in the biologically active defensins in wild cereals, in contrast to AMPs from cultivated forms that usually exhibit weak, if any, activity. We analyzed the similarity of various physicochemical parameters between thionins and defensins. The presence of a high divergence on a fixed part of any polypeptide that is close to defensins could be a determining factor. For all of the currently known hevein-like peptides of cereals, we can say that the determining factor in this regard is the structure of the chitin-binding domain, and in particular, amino acid residues that are not directly involved in intermolecular interaction with chitin. The analysis of amino acid sequences of alpha-hairpinins (hairpin-like peptides) demonstrated much higher antifungal activity and more specificity of the peptides from wild cereals compared with those from wheat and corn, which may be associated with the presence of a mini cluster of positively charged amino acid residues. In addition, at least one hydrophobic residue may be responsible for binding to the components of fungal cell membranes.

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Section: Investigation Of Structural Determinants Of Other Amps (Tmentioning

confidence: 89%

Primary Structure Analysis of Antifungal Peptides from Cultivated and Wild Cereals

Рогожин

Ryazantsev

Smirnov

et al. 2018

Plants

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“…Previously, we demonstrated that the native NsW2 peptide exhibited strong, dose-dependent cytotoxic activitiesagainst a range of tumor cell lines (AsPC-1, Colo357, RD, Jukart, HPF, B16, and HTC-116), with IC 50 values ranging from 0.2 to 0.4 µM [ 12 , 17 ]. Furthermore, the black seed thionin has been found to significantly suppress the transcriptional activity of certain oncogenes [ 26 ]. Therefore, it is of interest to compare trx-NsW2 with the previously obtained results.…”

Section: Discussionmentioning

confidence: 99%

Recombinant Fusion Protein Containing Plant Nigellothionin Regulates the Growth of Food-Spoiling Fungus (Aspergillus niger)

Barashkova,

Ryazantsev,

Zhuravleva

et al. 2023

Foods

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This study aimed to obtain a recombinant chimeric protein named trx-NsW2 via theheterologous expression of the multifunctional antimicrobial peptide nigellothionin from black cumin (Nigella sativa L.) seeds in the Escherichia coli system. The protein was purified using a combination of Ni-NTA affinity chromatography and reversed-phase HPLC. Based on the HPLC calibration, the total yield of the protein was calculated to be 650 mg/L of bacterial culture. The fungistatic activity of trx-NsW2 against the food-spoiling fungus Aspergillus niger was demonstrated as itinhibited the maturation of conidiawithout affecting conidial germination or fungal growth. In contrast to mature nigellothionin NsW2, the fusion protein showeda low level of cytotoxicity towards both normal and tumor cell lines at concentrationsof up to 100–200 µM. Interestingly, at lower concentrations, it even stimulated cytokinesis. These findings are of critical importance for applying chimeric antimicrobial proteins obtained via microbiological synthesis in applied science.

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“…We carried out a comparative study of the action of β-purothionin isolated from wheat germ, thionine isolated by E.A. Rogozhin from black cumin (Nigella sativa) seeds, and the crown compound in exposure of human cells to ionizing radiation [58]. Previously, we detected antimutagenic properties of β-purothionin [59] and the effect of this drug on the expression of genes involved in the tumorigenesis process.…”

Section: Approach To Reducing the Damaging Effects Of Radiation In Humentioning

confidence: 99%

The miRNA as human cell gene activity regulator after ionizing radiation

et al. 2017

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Abstract⎯This review presents analysis of the literature and our own research with respect to the role of miRNAs in the regulation of activity (expression) of genes controlling cellular homeostasis in human cells when exposed to ionizing radiation. Human cells, on one hand, can have increased resistance to radiation, which hinders the effectiveness of tumor treatment in radiotherapy. On the other hand, increased sensitivity to radiation may be accompanied by the development of several pathologies, including tumorigenesis. This paper examines the role of specific miRNAs in the formation of radioresistance and radiosensitivity of human cells and their impact on the respective target genes. Separate sections are devoted to the role of different miRNAs in radiation therapy of tumors of different localization, as well as their role in the bystander effect. A special section highlights features of gene activity and its regulators, miRNAs, in radiosensitive cells in patients with Down syndrome. The final section provides information about new approaches to change miRNA expression and, accordingly, their target genes by the action of plant and synthetic drugs (crown compounds) which reduce damaging effects of mutagens. It is assumed that antimutagens affecting the expression levels of miRNAs and structural genes may be used to correct the increase and decrease in cellular radioresponse, reducing the risk of development of pathological processes, including tumorigenesis.

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Comparative analysis of natural and synthetic antimutagens as regulators of gene expression in human cells under exposure to ionizing radiation

Cited by 4 publications

References 24 publications

Primary Structure Analysis of Antifungal Peptides from Cultivated and Wild Cereals

Primary Structure Analysis of Antifungal Peptides from Cultivated and Wild Cereals

Recombinant Fusion Protein Containing Plant Nigellothionin Regulates the Growth of Food-Spoiling Fungus (Aspergillus niger)

The miRNA as human cell gene activity regulator after ionizing radiation

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