Comparative Analysis of Number and Competence in Meiotic Maturation of Preovulatory Oocytes of C57Bl/6J Mice and Their F1 Hybrids after Stimulation with Gonadotropin

Riabukha, Ya A; Petrova, Dorina; Зацепина, О. В.

doi:10.1007/s10517-019-04603-z

Cited by 3 publications

(1 citation statement)

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“…To harvest cells from oviducts, we had to induce ovulation through hormonal stimulation, which was not used in the fertility trials or histology. To our knowledge, hormone stimulation does not negatively impact meiotic maturation [ 37 ]. In this strain background, ~80% of cells in oviducts from WT mice contained polar bodies ( Fig 3A and 3B ), indicating a completion of meiosis I (MI) and arrest at Metaphase of meiosis II (Met II).…”

Section: Resultsmentioning

confidence: 99%

Aurora kinase A is essential for meiosis in mouse oocytes

et al. 2021

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The Aurora protein kinases are well-established regulators of spindle building and chromosome segregation in mitotic and meiotic cells. In mouse oocytes, there is significant Aurora kinase A (AURKA) compensatory abilities when the other Aurora kinase homologs are deleted. Whether the other homologs, AURKB or AURKC can compensate for loss of AURKA is not known. Using a conditional mouse oocyte knockout model, we demonstrate that this compensation is not reciprocal because female oocyte-specific knockout mice are sterile, and their oocytes fail to complete meiosis I. In determining AURKA-specific functions, we demonstrate that its first meiotic requirement is to activate Polo-like kinase 1 at acentriolar microtubule organizing centers (aMTOCs; meiotic spindle poles). This activation induces fragmentation of the aMTOCs, a step essential for building a bipolar spindle. We also show that AURKA is required for regulating localization of TACC3, another protein required for spindle building. We conclude that AURKA has multiple functions essential to completing MI that are distinct from AURKB and AURKC.

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Section: Resultsmentioning

confidence: 99%