Comparative analysis of peptidoglycan recognition proteins in endoparasitoid wasp <i>Microplitis mediator</i>

Wang, Rui‐Juan; Zhe, Liu; Jiang, Hong; Li, Jiancheng; Saha, Tusar T.; Lu, Z.-X.; Lu, Zhiqiang; Zou, Zhen

doi:10.1111/1744-7917.12290

Cited by 16 publications

(7 citation statements)

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“…This indicated that BdPGRP-SA can be induced by E. coli and S. aureus (Figure 4). The inducible expression of PGRPs has been observed in Armigeres subalbatus [45], A. aegypti [41], and Microplitis mediator [46]. The induction of BdPGRP-SA and -SD by PGN-EB was confirmed earlier [47].…”

Section: Discussionmentioning

confidence: 65%

Characterization and Function of Two Short Peptidoglycan Recognition Proteins Involved in the Immunity of Bactrocera dorsalis (Hendel)

Wei

Liu

Zhang

et al. 2019

Insects

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Peptidoglycans (PGNs) are major bacterial components recognized by the immune systems of insects and mammals. PGN recognition proteins (PGRPs) are widely distributed and highly conserved in vertebrates and invertebrates. PGRPs are a family of pattern recognition receptors that recognize peptidoglycan and regulate immune responses. In this study, we cloned two PGRP genes (BdPGRP-SA and BdPGRP-SD) from Bactrocera dorsalis (Hendel), which encode 192 and 196 amino acid residues, respectively. Both genes were highly expressed in adults, especially in the fat body and midgut. These two genes were up-regulated when challenged by the immune triggers, PGN-EB (Escherichia coli O111:B4) and PGN-SA (Staphylococcus aureus). The suppression of transcriptional expression of either gene by RNA interference (RNAi) resulted in increased sensitivities to Gram-negative E. coli and Gram-positive S. aureus PGNs. Suppression of BdPGRP-SA and -SD expression by RNAi resulted in weak expressions of four antimicrobial peptides (AMPs) upon injected with E. coli or S. aureus. BdPGRP-SA and -SD are involved in recognizing both Gram-negative and Gram-positive bacteria independently to activate the downstream AMP’s response to bacterial infection.

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Section: Discussionmentioning

confidence: 65%

Characterization and Function of Two Short Peptidoglycan Recognition Proteins Involved in the Immunity of Bactrocera dorsalis (Hendel)

Wei

Liu

Zhang

et al. 2019

Insects

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“…dsRNA was synthesized from specific gene templates according to a previously described method ( 62 ). The GFP gene was used as the control.…”

Section: Methodsmentioning

confidence: 99%

A Metalloprotease Homolog Venom Protein From a Parasitoid Wasp Suppresses the Toll Pathway in Host Hemocytes

et al. 2018

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Parasitoid wasps depend on a variety of maternal virulence factors to ensure successful parasitism. Encapsulation response carried out by host hemocytes is one of the major host immune responses toward limiting endoparasitoid wasp offspring production. We found that VRF1, a metalloprotease homolog venom protein identified from the endoparasitoid wasp, Microplitis mediator, could modulate egg encapsulation in its host, the cotton bollworm, Helicoverpa armigera. Here, we show that the VRF1 proenzyme is cleaved after parasitism, and that the C-terminal fragment containing the catalytic domain enters host hemocytes 6 h post-parasitism. Furthermore, using yeast two-hybrid and pull-down assays, VRF1 is shown to interact with the H. armigera NF-κB factor, Dorsal. We also show that overexpressed of VRF1 in an H. armigera cell line cleaved Dorsal in vivo. Taken together, our results have revealed a novel mechanism by which a component of endoparasitoid wasp venom interferes with the Toll signaling pathway in the host hemocytes.

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“…The phylogenetic tree was constructed using the neighbour-joining algorithm, enger receptors (Yu et al, 2002;Lemaitre & Hoffmann, 2007;Ragan et al, 2009;Sumathipala & Jiang, 2010;Bang et al, 2013Bang et al, , 2022. As a member of the PRP family, PGRP only recognizes molecules on the surface of pathogenic organisms and then selectively activates pathways such as the Toll pathway, immune defi ciency (IMD) pathway, JAK-STAT pathway and reactive oxygen species metabolism or blackening reaction to kill the pathogens Huang et al, 2015;Wang et al, 2016;Lu et al, 2019;Hou et al, 2020;Wei et al, 2020).…”

Section: Phylogenetic Analysismentioning

confidence: 99%

Screening of immune-related genes against bacterial infection in Ostrinia furnacalis (Lepidoptera: Crambidae)

Zhou¹,

Wang²,

Baohong³

2022

Eur. J. Entomol.

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http://www.eje.cz can kill Asian corn borer it has an immune response that enable it to resist and kill the invading pathogenic microorganisms. However, the detailed molecular mechanism of the interaction between O. furnacalis and pathogenic bacteria has not yet been clarifi ed and elucidating this mechanism can help to better exploit the lethal effect of pathogenic microorganisms by restraining the natural immune response of O. furnacalis. Therefore, the screening and identifi cation of immune-related genes are important for understanding the natural immune system in O. furnacalis and improving the effi ciency of biocontrol techniques.Insects defend themselves against pathogen infestation by immune responses and the recognition of pathogens is the fi rst step in initiating this response (Kanost et al., 2004; Vogel et al., 2011). Insects recognize pathogens by means of pattern recognition proteins (PRPs) (Hughes, 2012;Wang et al., 2019). The classes of insect PRPs identifi ed to date and the corresponding pathogen pattern molecules include peptidoglycan recognition proteins, β-1,3-glucan recognition protein, C-type lectins, fi brinogen-related proteins, thioester-containing proteins and macrophage scav-

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Comparative analysis of peptidoglycan recognition proteins in endoparasitoid wasp Microplitis mediator

Cited by 16 publications

References 50 publications

Characterization and Function of Two Short Peptidoglycan Recognition Proteins Involved in the Immunity of Bactrocera dorsalis (Hendel)

Characterization and Function of Two Short Peptidoglycan Recognition Proteins Involved in the Immunity of Bactrocera dorsalis (Hendel)

A Metalloprotease Homolog Venom Protein From a Parasitoid Wasp Suppresses the Toll Pathway in Host Hemocytes

Screening of immune-related genes against bacterial infection in Ostrinia furnacalis (Lepidoptera: Crambidae)

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