Comparative analysis of PIA and rSesC mixture, arisen antibodies against the biofilm forming Staphylococcus aureus.

Abstract

Background: Staphylococcus aureus as a causative agent of hospital-acquired infections, has been considered as the primary concern in biomaterial-related infections (BAIs). Following the purification of polysaccharide intercellular adhesion (PIA) as an efficient macromolecule in biofilm formation in the native condition, recombinant S . epidermidis surface exposed rSesC protein, with the most homology to clumping factor A (ClfA) in S. aureus was cloned and expressed in a prokaryotic host as well.Fourier transform infrared spectrometry (FTIR) and Western blotting procedure analyzed purified PIA and protein, respectively. Then, the immune response was evaluated by measuring total IgG titers.Moreover, the capacity of Anti-biofilm forming activity of arisen antibodies to a biofilm forming S. aureus strains was assessed by semi-quantitative micro-plate procedure.Results: Data showed that the total IgGs was boosted in mice immunized sera. By performing inhibition assay, biofilm inhibitory effect of secreted antibodies to test strain was observed. Arisen antibodies against the mixture significantly were more potent than PIA and rSesC, when comparing them in a biofilm inhibition assay. Conclusion: Immunization of mice with mentioned antigens especially a mixture of them, could eliminate the biofilm formation process in S. aureus . Hopefully, this study corresponds the suggestion that, the immunization of mice with PIA and rSesC candidate vaccine could protect against S. aureus infection. Background Staphylococci are opportunistic pathogens and determined as the most common causes of infections related to implanted medical devices, infect both hospitalized patients and immunocompromised individuals [1].Considering diverse virulence factors, such as capsule and cell wall-bound adhesion molecules, surface proteins, toxins, antibiotic resistance, and biofilm formation cause infections in human and animal. [2]. S. aureus is an etiological agent of the mild to severe infections in hospitalized patients, such as bacteremia due to endocarditis, pneumonia, and metastatic infections.Finding revealed that, majority of adults are either permanently or transiently susceptible to colonization by S. aureus. [[3] Up to 20-30% of humans, asymptomatically are colonized by S. aureus