Comparative analysis of proteinase, phospholipase, hydrophobicity and biofilm forming ability in Candida species isolated from clinical specimens

Dabiri, Shima; Shams-Ghahfarokhi, Masoomeh; Razzaghi-Abyaneh, Mehdi

doi:10.1016/j.mycmed.2018.04.009

Cited by 46 publications

(36 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, in case of proteinase enzyme activity it was shown that C. glabrata was the lowest proteinase producer as 40.0% (4/10) showed only weak enzymatic activity and 60.0% (6/10) were negative followed by C. tropicalisas 63.0% (17/27) showed weak enzymatic activity and 37.0% (10/27) were negative. However the highest enzymatic activity were reported for C. albicans isolates as 84.6% (11/13) showed strong enzymatic activity, 7.7% (1/13) showed moderate activity and 7.7% (1/13) showed weak activity with highly statistical significant differences (P<0.001), in agreement with the results of Dabiri et al, 2018 26 who reported that C. albicans showed the highest proteinase and phospholipase activity and biofilm formation ability. And Alenzi, 2016 27 founded that Secretory aspartic proteinase activity was demonstrated in 48.0% of Candida isolates.…”

Section: Resultssupporting

confidence: 89%

“…However, 9/10 (90%) of C. glabrata were weak biofilm producer (+) and 1/10 (10%) was negative biofilm producer (-) with highly statistical significant difference (P<0.001). Table (5). ** P value highly significant 26 who founded that the highest positivity of phospholipase production was related to C. albicans 56.8% followed by C. tropicalis 50.0% and C. glabrata isolates 20.0%.…”

Section: Resultsmentioning

confidence: 95%

See 1 more Smart Citation

Assessment of virulence factors and antifungal susceptibility of Candida species isolated from catheter associated urinary tract infections

Ismail

El-Haliem

Aboelmagd

2020

Al-Azhar International Medical Journal

View full text Add to dashboard Cite

Background: Catheter associated urinary tract infection (CAUTI) is a crucial attribute to virulence of organisms. Emergency of non albicans Candida (NAC) species become common due to several virulence factors, which play an important role in pathogenicity and their resistance to treatment. Aim of the work: To investigate different virulence factors of Candida spp. isolated from CAUTI patients and evaluate their antifungal susceptibility. Patients and Methods: Urine samples collected from 219 indwelling catheters of ICU patients. Candida isolates identified using Germ tube test, Chrom ID agar, Corn meal tween agar, VITEK 2 system and Integral System Yeast Plus (ISYP) used to detect antifungal susceptibility. Enzymatic, haemolytic activity and biofilm formation assessed by standard methods. Results: The incidence of candiduria was 50/219 (22.8%). Candida tropicalis was predominant one (54.0%) followed by C. albicans (26.0%) and C. glabrata (20.0%). Females gender (64.0%), Diabetes (50.0%) associated with positive candiduria, while antibiotic therapy was predominant risk factor (78.0%). C. albicans have highest enzymatic activity for phospholipase (84.6 %), proteinase (84.6%) and haemolytic activity (100%), while C. tropicalis have highest biofilm formation (100%). NAC spp. showed higher rates of resistance to azole drugs (Clotrimazole 77.6%, Miconazole 70.3%, Econazole 63.2%, Nystatine 44.3%), while C. albicans didn't show any resistance. Conclusion: Candiduria is prevalent among catheterized patients with shift toward NAC spp. causing nosocomial UTI. A strong relationship between host risk factors (old age, antibiotic use, catheterization, female sex, ICU stay, diabetes) and expression of various virulence factors of Candida spp. causing candiduria and resistance to antifungal drugs.

show abstract

Section: Resultssupporting

confidence: 89%

Section: Resultsmentioning

confidence: 95%

Assessment of virulence factors and antifungal susceptibility of Candida species isolated from catheter associated urinary tract infections

Ismail

El-Haliem

Aboelmagd

2020

Al-Azhar International Medical Journal

View full text Add to dashboard Cite

show abstract

“…This finding is similar to that previously reported for strains from wild (Brilhante et al 2013;Lord et al 2010) or domesticated/pet birds (Subramanya et al 2017;Ying and Chunyang 2012). The production of phospholipase and proteinase has been shown to play a significant role in the pathogenesis and virulence of Candida species and related diseases (Dabiri et al 2018). In addition, multidrug resistant yeasts often cause fatal infection in immunocompromised hosts (Mellinghoff et al 2018).…”

Section: Discussionsupporting

confidence: 87%

Virulence and in vitro antifungal susceptibility of Candida albicans and Candida catenulata from laying hens

et al. 2020

View full text Add to dashboard Cite

In spite of evidence that domestic and wild birds may act as carriers of human pathogenic fungi, data on the role of laying hens as reservoirs of drug resistant and virulent yeasts is lacking. Here, we assess several virulence factors (phospholipase and haemolysin activity) and the antifungal susceptibility profiles of 84 Candida albicans and 17 Candida catenulata strains isolated from cloacae (group A), faeces (group B) and eggs (group C) of laying hens. Of these strains, 95% C. albicans and 23% C. catenulata strains displayed phospholipase and haemolytic activities. For C. albicans, the highest values of phospholipase (Pz = 0.62) and haemolytic activities (Hz = 0.49) were recorded among the strains from group C whilst for C. catenulata (Pz = 0.54; Hz = 0.49) among those from group A. High minimum inhibitory concentration (MIC) values for azoles and amphotericin B (AmB) were recorded irrespective of their sources in all C. albicans strains. A total of 22 C. albicans strains were multidrug resistant, displaying resistance to fluconazole, itraconazole (ITZ), voriconazole (VOR) and posaconazole (POS). All C. catenulata strains from group C were resistant to ITZ, POS, micafungin and anidulafungin and susceptible to AmB. In this study, C. albicans and C. catenulata isolated from the cloacae, faeces and eggs of laying hens produced phospholipase and haemolysin and might be multidrug resistant. In the environment (faeces) or in eggs, C. albicans and C. catenulata strains might acquire pathogenic virulence traits and/or show multidrug resistance profiles. Based on these results, breeding and handling of laying hens and/or eggs may have implications for human and animal health.

show abstract

“…Moreover, lipase disruptant mutants of C. albicans and C. parapsilosis were shown to be significantly less virulent in a rat model of neonatal candidiasis [56]. A comparative analysis of multiple pathogenic Candida species demonstrated that C. albicans has the highest phospholipase activity [57]. Although C. auris has a similar amount of lipase encoding genes in its genome as C. albicans [24], the ability to produce phospholipases seems to be reduced and strain-dependent [48].…”

Section: Attacking the Host: Lytic Enzyme Production And Secretionmentioning

confidence: 99%

“…Multiple studies showed that C. albicans has the highest biofilm forming ability compared to other pathogenic Candida species [32,57,61]. Also C. auris strains form significantly reduced biofilms compared to C. albicans [32,48].…”

Section: Defense Against Antifungals: Intrinsic Resistance Of Biofilmsmentioning

confidence: 99%

Attack, Defend and Persist: How the Fungal Pathogen Candida auris was Able to Emerge Globally in Healthcare Environments

Jong

Hagen

2019

Mycopathologia

View full text Add to dashboard Cite

Within a decade after its first description, the multidrug-resistant yeast Candida auris has emerged globally as a nosocomial pathogen causing difficult to control outbreaks. This, together with the alarmingly high mortality rate of up to 66% associated with C. auris candidemia, calls for a better understanding of its virulence traits and routes of transmission. Unlike other clinically relevant Candida species, C. auris seems to have the unique ability to be easily transmitted between patients. Although initially thought to express fewer virulence traits than Candida albicans, recent genomic insights suggest C. auris to possess these traits to a much more similar extent. This review highlights the virulence traits C. auris expresses to attack the host, defend itself against antimicrobial agents and to persist within the healthcare environment.

show abstract

Comparative analysis of proteinase, phospholipase, hydrophobicity and biofilm forming ability in Candida species isolated from clinical specimens

Cited by 46 publications

References 30 publications

Assessment of virulence factors and antifungal susceptibility of Candida species isolated from catheter associated urinary tract infections

Assessment of virulence factors and antifungal susceptibility of Candida species isolated from catheter associated urinary tract infections

Virulence and in vitro antifungal susceptibility of Candida albicans and Candida catenulata from laying hens

Attack, Defend and Persist: How the Fungal Pathogen Candida auris was Able to Emerge Globally in Healthcare Environments

Contact Info

Product

Resources

About