Comparative analysis of QS3D versus droplet digital PCR for quantitative measures of EGFR T790M mutation from identical plasma

Guo, Qiaojuan; Wang, Lin; Liang, Xiaohui; Zhao, Mingna; Huang, Xu; Xu, Wanxing; Lou, Jiatao; Qiao, Lihua

doi:10.1016/j.heliyon.2022.e11339

Heliyon

2022

DOI: 10.1016/j.heliyon.2022.e11339

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Comparative analysis of QS3D versus droplet digital PCR for quantitative measures of EGFR T790M mutation from identical plasma

Qiaojuan Guo¹,

Lin Wang²,

Xiaohui Liang³

et al.

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2024

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Cited by 3 publications

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Advances in genotypic antimicrobialresistance testing: a comprehensive review

Duan,

Zeng,

Peng

2024

Sci. China Life Sci.

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Advances in genotypic antimicrobialresistance testing: a comprehensive review

Duan,

Zeng,

Peng

2024

Sci. China Life Sci.

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Applications of Digital Polymerase Chain Reaction (dPCR) in Molecular and Clinical Testing

Wainman,

Sathyanarayana,

Lefferts

2024

The Journal of Applied Laboratory Medicine

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Background Digital polymerase chain reaction (dPCR) is an accurate and sensitive molecular method that can be used in clinical diagnostic, prognostic, and predictive tests. The key component of the dPCR method is the partitioning of a single reaction into many thousands of droplets, nanochannels or other nano- or picoliter-sized reactions. This results in high enough sensitivity to detect rare nucleic acid targets and provides an absolute quantification of target sequences or alleles compared to other PCR-based methods. Content An increasing number of dPCR platforms have been introduced commercially in recent years and more are being developed. These platforms differ in the method of partitioning, degree of automation, and multiplexing capabilities but all can be used in similar ways for sensitive and highly accurate quantification of a variety of nucleic acid targets. Currently, clinical applications of dPCR include oncology, microbiology and infectious disease, genetics, and prenatal/newborn screening. Commercially available tests for clinical applications are being developed for variants with diagnostic, prognostic, and therapeutic significance in specific disease types. Summary The power of dPCR technology relies on the partitioning of the reactions and results in increased sensitivity and accuracy compared to qPCR. More recently, the sensitivity of dPCR has been applied to the detection of known variants in cell-free DNA and circulating tumor DNA. Future clinical applications of dPCR include liquid biopsy, treatment resistance detection, screening for minimal residual disease, and monitoring allograft engraftment in transplanted patients.

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Advancing Pathogen Identification: The Role of Digital PCR in Enhancing Diagnostic Power in Different Settings

Mirabile,

Sangiorgio,

Bonacci

et al. 2024

Diagnostics

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Digital polymerase chain reaction (dPCR) has emerged as a groundbreaking technology in molecular biology and diagnostics, offering exceptional precision and sensitivity in nucleic acid detection and quantification. This review highlights the core principles and transformative potential of dPCR, particularly in infectious disease diagnostics and environmental surveillance. Emphasizing its evolution from traditional PCR, dPCR provides accurate absolute quantification of target nucleic acids through advanced partitioning techniques. The review addresses the significant impact of dPCR in sepsis diagnosis and management, showcasing its superior sensitivity and specificity in early pathogen detection and identification of drug-resistant genes. Despite its advantages, challenges such as optimization of experimental conditions, standardization of data analysis workflows, and high costs are discussed. Furthermore, we compare various commercially available dPCR platforms, detailing their features and applications in clinical and research settings. Additionally, the review explores dPCR’s role in water microbiology, particularly in wastewater surveillance and monitoring of waterborne pathogens, underscoring its importance in public health protection. In conclusion, future prospects of dPCR, including methodological optimization, integration with innovative technologies, and expansion into new sectors like metagenomics, are explored.

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Comparative analysis of QS3D versus droplet digital PCR for quantitative measures of EGFR T790M mutation from identical plasma

Cited by 3 publications

References 22 publications

Advances in genotypic antimicrobialresistance testing: a comprehensive review

Advances in genotypic antimicrobialresistance testing: a comprehensive review

Applications of Digital Polymerase Chain Reaction (dPCR) in Molecular and Clinical Testing

Advancing Pathogen Identification: The Role of Digital PCR in Enhancing Diagnostic Power in Different Settings

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