Comparative analysis of restraint stress-induced depressive-like phenotypes in C57BL/6N mice derived from three different sources

Abstract

C57BL/6NKorl mice are a novel mouse stock recently developed by the National Institute of Food and Drug Safety Evaluation in Korea. Extensive research into the nature of C57BL/6NKorl mice is being conducted. However, there is no scientific evidence for the phenotypic response to restraint stress (RST), a stress paradigm for modeling depressive disorders, in rodents. In this study, we investigated the repeated RST-induced depressive-like phenotypes in C57BL/6 N mouse substrains (viz., C57BL/6NKorl mice from Korea, C57BL/6NA mice from the United States, and C57BL/6NB mice from Japan) obtained from different sources. The results showed that C57BL/6 N mice derived from various sources exposed to repeated RST resulted in depressive-like phenotypes reflected by a similar degree of behavioral modification and susceptibility to oxidative stress in a duration-dependent manner, except for the distinctive features (increased body weight (BW) and tolerance to the suppression of BW gain by exposure to repeated RST) in C57BL/6NKorl mice. Taken together, the duration-dependent alteration in depressive-like phenotypes by repeated exposure to RST observed in this study may provide valuable insights into the nature of C57BL/6NKorl mice as an alternative animal resource for better understanding of the etiology of depressive disorders and the mechanisms of antidepressant actions.

Inflammatory responses of C57BL/6NKorl mice to dextran sulfate sodium-induced colitis: comparison between three C57BL/6 N sub-strains

“…Prior to DSS treatment, C57BL/6NKorl mice had higher body weights compared to the other two sub-strains of C57BL/6 N mice (Fig. 1 a), in agreement with previous studies [ 9 , 10 , 12 , 14 ]. DSS caused dose- and time-dependent body weight loss (Fig.…”

“…The characteristics of C57BL/6NKorl have been identified by comparing these mice to the generally used C57BL/6 N sub-strains from different sources. The C57BL/6NKorl mice have shown similar humoral immunity, fertilization and embryo development rates, tumorigenesis, and responses to cisplatin and restraint stress compared to other C57BL/6 N mice [ 9 – 14 ]. However, relatively higher body weight and adipose tissue mass, and lower oxygen consumption were found to be distinct features of the C57BL/6NKorl sub-strain [ 9 , 10 , 12 , 14 ].…”

“…The C57BL/6NKorl mice have shown similar humoral immunity, fertilization and embryo development rates, tumorigenesis, and responses to cisplatin and restraint stress compared to other C57BL/6 N mice [ 9 – 14 ]. However, relatively higher body weight and adipose tissue mass, and lower oxygen consumption were found to be distinct features of the C57BL/6NKorl sub-strain [ 9 , 10 , 12 , 14 ]. Moreover, cell-mediated immune response of C57BL/6NKorl mice determined by concanavalin A-induced splenic T cell proliferation was stronger than those of other C57BL/6 N sub-strains [ 11 ].…”

“…Recently, a new sub-strain of C57BL/6 N mice, named C57BL/6NKorl, was established by the National Institute of Food and Drug Safety Evaluation (NIFDS) in Korea. To identify their characteristics, the physical and biological traits of these mice have been tested and compared with those of the previously used C57BL/6 N mice [ 9 – 14 ]. However, the inflammatory responses of this sub-strain to DSS-induced colitis have not yet been examined.…”

Reduced sociability and social agency encoding in adult Shank3-mutant mice are restored through gene re-expression in real time

“…The blood was centrifuged and plasma was collected and stored at −80°C. Plasma corticosterone levels were measured using an ELISA kit according to the manufacture’s protocol (ADI-900-097; Enzo Life Sciences) and a microplate reader at 405nm (Absorbance 96; Enzo Life Sciences) 57 , 58 . Contaminated samples were excluded.…”