Comparative analysis of surface wax in mature fruits between Satsuma mandarin (Citrus unshiu) and ‘Newhall’ navel orange (Citrus sinensis) from the perspective of crystal morphology, chemical composition and key gene expression

Wang, Jinqiu; Hao, Haohao; Liu, Runsheng; Ma, Qiaoli; Xu, Juan; Chen, Feng; Cheng, Yunjiang; Deng, Xiuxin

doi:10.1016/j.foodchem.2013.12.021

Cited by 105 publications

(107 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3). Similar gradients between both of the wax layers, also with cyclics that were restricted to the internal part of the cuticle, had previously been reported for leaves of Prunus laurocerasus (Jetter et al 2000) and Ligustrum vulgare (Buschhaus et al 2007), fruits of tomato (Vogg et al 2004) and citrus (Wang et al 2014). Considering all of these results together, our present data further support the hypothesis that cyclics tend to accumulate in the plant intracuticular compartment.…”

Section: Aliphatic Compounds Dominated the Epicuticular Waxes Whereasupporting

confidence: 91%

“…The results showed that the epicuticular waxes of both cultivars were mostly composed of aldehydes, alkanes, fatty acids, and primary alcohols. The epicuticular wax accounted for 80 % of the total wax in the Newhall fruits, which was higher than that in Satsuma fruits (Wang et al 2014).…”

Section: Introductionmentioning

confidence: 72%

“…As a result, the wax mixtures that were used for these studies probably contained the total waxes rather than only the epicuticular waxes. Only recently have the epicuticular waxes of citrus been sampled separately and analyzed by Wang et al (2014). To our knowledge, 'glossy Newhall' (MT) is the only glossy mutant of citrus whose cuticular wax composition has been characterized, as in our previous report (Liu et al 2012).…”

Section: Aliphatic Compounds Dominated the Epicuticular Waxes Whereamentioning

confidence: 88%

“…In summary, alkanes, aldehydes, primary alcohols, fatty acids and triterpenoids are the major wax fractions in citrus fruits (Albrigo 1972;Baker et al 1975;Freeman et al 1979;El-Otmani and Coggins 1985;El-Otmani et al 1986;Sala et al 1992;McDonald et al 1993;Nordby and McDonald 1994;Sala 2000;Cajuste et al 2010;Liu et al 2012;Wang et al 2014). However, in most previous studies, fruit waxes were extracted by dipping the intact surface of an organ into organic solvents.…”

Section: Aliphatic Compounds Dominated the Epicuticular Waxes Whereamentioning

confidence: 99%

“…For a number of plant species, the two wax layers contain drastically different compositions. Aliphatic wax compounds, such as aldehydes, alkanes, primary alcohols, secondary alcohols and ketones, were enriched in epicuticular waxes, whereas cyclics, including terpenoids and sterols, were mainly present in intracuticular waxes (Vogg et al 2004;Gniwotta et al 2005;Wen et al 2006;Jetter et al 2006;Wang et al 2014).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Analysis of cuticular wax constituents and genes that contribute to the formation of ‘glossy Newhall’, a spontaneous bud mutant from the wild-type ‘Newhall’ navel orange

et al. 2015

View full text Add to dashboard Cite

Navel orange (Citrus sinensis [L.] Osbeck) fruit surfaces contain substantial quantities of cuticular waxes, which have important eco-physiological roles, such as water retention and pathogen defense. The wax constituents of ripe navel orange have been studied in various reports, while the wax changes occurring during fruit development and the molecular mechanism underlying their biosynthesis/export have not been investigated. Recently, we reported a spontaneous bud mutant from the wild-type (WT) 'Newhall' Navel orange. This mutant displayed unusual glossy fruit peels and was named 'glossy Newhall' (MT). In this study, we compared the developmental profiles of the epicuticular and intracuticular waxes on the WT and MT fruit surfaces. The formation of epicuticular wax crystals on the navel orange surface was shown to be dependent on the accumulation of high amounts of aliphatic wax components with trace amounts of terpenoids. In sharp contrast, the underlying intracuticular wax layers have relatively low concentrations of aliphatic wax components but high concentrations of cyclic wax compounds, especially terpenoids at the late fruit developmental stages. Our work also showed that many genes that are involved in wax biosynthesis and export pathways were down-regulated in MT fruit peels, leading to a decrease in aliphatic wax component amounts and the loss of epicuticular wax crystals, ultimately causing the glossy phenotype of MT fruits.

show abstract

Section: Aliphatic Compounds Dominated the Epicuticular Waxes Whereasupporting

confidence: 91%

Section: Introductionmentioning

confidence: 72%

Section: Aliphatic Compounds Dominated the Epicuticular Waxes Whereamentioning

confidence: 88%

Section: Aliphatic Compounds Dominated the Epicuticular Waxes Whereamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Analysis of cuticular wax constituents and genes that contribute to the formation of ‘glossy Newhall’, a spontaneous bud mutant from the wild-type ‘Newhall’ navel orange

et al. 2015

View full text Add to dashboard Cite

show abstract

Effects of cuticular wax on the postharvest physiology in fragrant pear at different storages

Mao¹,

Chen

et al. 2022

J Sci Food Agric

View full text Add to dashboard Cite

BACKGROUND Epidermal wax is an important factor affecting the storage quality of fruits and vegetables. Previous studies have shown that the epidermal wax of fruits undergoes significant changes during storage, but there are few studies on the effects of different storage methods on the changes in waxes and the relationship with storage quality. To investigate the effect of cuticular wax on the postharvest physiology in fragrant pear, equal numbers of fragrant pear fruits were stored in room temperature storage (control), cold storage and controlled atmosphere (CA) storage environs, respectively. RESULTS Gas chromatography‐mass spectrometry analysis revealed that the prevailing compositions of cuticular wax of fragrant pear were alkanes, alkenes, alcohols, aldehydes, esters and fatty acids. Compared with the control, cold storage and CA storage significantly inhibited changes in postharvest physiology, total wax contents and wax compositions of fragrant pear, and the effects of CA storage were more pronounced than cold storage. Under different storage methods, total wax contents and wax compositions show different correlations with various physiological indicators. CONCLUSION The results obtained in the present study indicate that cold storage and CA storage altered the fragrant pear cuticular wax contents and constituents, thus changing the postharvest physiology quality. The changes in the metabolism of wax components caused by the changes in storage environment mainly affect the changes in the hardness of fragrant pears. The present study provides a theoretical basis for the preservation and storage of fruits. © 2022 Society of Chemical Industry

show abstract

Effects of 1‐methylcyclopropene on surface wax and related gene expression in cold‐stored ‘Hongxiangsu’ pears

2018

View full text Add to dashboard Cite

BACKGROUND: Surface wax protects fruit from dehydration and pathogen erosion during storage. The surface wax of pears changes greatly during storage. In this work, the effect of ethylene action inhibitor 1-methylcyclopropene on wax accumulation and related gene expression in 'Hongxiangsu' pears during cold storage was investigated. RESULTS: The alkanes, alkenes, fatty acids, esters, aldehydes and triterpenoids on the fruit surface accumulated and peaked at day 180, but fatty alcohols decreased before day 90 and then increased in the control. Treatment with 1-MCP (1.0 L L −1 ) reduced surface wax at day 180 of storage. Compared with the control, the wax crystals became smaller in 1-MCP-treated fruit on days 90 and 270. The 1-MCP decreased the expression levels of ethylene synthesis, perception and signal genes ACS1, ACO1, ERS1, ETR2, ERF1 and wax-related genes (LACS1, LACS2, KCS2, KCS9, KCS20, FDH, CER6, CER10, LTPG1, LTP3, LTP4, ABCG11 and ABCG12). CONCLUSION: These results suggested that 1-MCP suppressed ethylene synthesis and signal-pathway and wax-related gene expression; it also reduced the wax and the size of crystals on the fruit surface in cold-stored 'Hongxiangsu' pears. /jsfa ± 176.77b 1277.69 ± 124.08c 3012.26 ± 534.27a 680.31 ± 109.6c 3549.66 ± 760.88a 1201.3 ± 94.57c 1136.8 ± 121.64c 794.82 ± 119.56c J Sci Food Agric 2019; 99: 2438-2446

show abstract

Comparative analysis of surface wax in mature fruits between Satsuma mandarin (Citrus unshiu) and ‘Newhall’ navel orange (Citrus sinensis) from the perspective of crystal morphology, chemical composition and key gene expression

Cited by 105 publications

References 29 publications

Analysis of cuticular wax constituents and genes that contribute to the formation of ‘glossy Newhall’, a spontaneous bud mutant from the wild-type ‘Newhall’ navel orange

Analysis of cuticular wax constituents and genes that contribute to the formation of ‘glossy Newhall’, a spontaneous bud mutant from the wild-type ‘Newhall’ navel orange

Effects of cuticular wax on the postharvest physiology in fragrant pear at different storages

Effects of 1‐methylcyclopropene on surface wax and related gene expression in cold‐stored ‘Hongxiangsu’ pears

Contact Info

Product

Resources

About