Comparative analysis of temperature preference behavior and effects of temperature on daily behavior in 11 Drosophila species

Ito, Fumihiro; Awasaki, Takeshi

doi:10.1038/s41598-022-16897-7

Cited by 21 publications

(20 citation statements)

References 49 publications

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“…Importantly, the temperature, was stable, reproducible, and linear, with a slope of 0.6°C/cm, as previously described [42]. Previous studies have evaluated thermal preference in groups of 10-30 flies that are monitored for 20-30 min [27; 43; 44]. However, we prefer studying individual flies in which 1) the sleep history of that individual has been well characterized, and 2) the individual fly can be retrieved at the end of the assay and their behavior can be further evaluated [39; 45].…”

Section: Resultsmentioning

confidence: 95%

“…C) Temperature readings in 1 cm increments measured using an infrared Thermometer (4 independent samples were taken at each location). D) Temperature preference in Canton-S (Cs) flies after being placed into the apparatus for different time intervals (n=11 flies/interval); One way ANOVA: F [3,43] = 1.36; p=0.26. E) Temperature preference for Cs flies tested in the morning 8:30-10:30am, n=12; afternoon 24pm, n=11; evening 6-8pm; One way ANOVA for condition: F [2,34] = 57.4; p=2.6 E-11 *p<0.05, corrected Bonferroni test.…”

Section: Methodsmentioning

confidence: 99%

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Sleep Deprivation, Sleep Fragmentation and Social Jet Lag increase temperature preference inDrosophila

Roach

Ford

Simhambhatla

et al. 2023

Preprint

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Despite the fact that sleep deprivation substantially affects the way animals regulate their body temperature, the specific mechanisms behind this phenomenon are not well understood. In both mammals and flies, neural circuits regulating sleep and thermoregulation overlap, suggesting an interdependence that may be relevant for sleep function. To investigate this relationship further, we exposed flies to 12 h of sleep deprivation, or 48 h of sleep fragmentation and evaluated temperature preference in a thermal gradient. Flies exposed to 12 h of sleep deprivation chose warmer temperatures after sleep deprivation. Importantly, sleep fragmentation, which prevents flies from entering deeper stages of sleep, but does not activate sleep homeostatic mechanisms nor induce impairments in short-term memory also resulted in flies choosing warmer temperatures. To identify the underlying neuronal circuits, we used RNAi to knock down the receptor forPigment dispersing factor, a peptide that influences circadian rhythms, temperature preference and sleep. Expressing UAS-PdfrRNAiin subsets of clock neurons prevented sleep fragmentation from increasing temperature preference. Finally, we evaluated temperature preference after flies had undergone a social jet lag protocol which is known to disrupt clock neurons. In this protocol, flies experience a 3 h light phase delay on Friday followed by a 3 h light advance on Sunday evening. Flies exposed to social jet lag exhibited an increase in temperature preference which persisted for several days. Our findings identify specific clock neurons that are modulated by sleep disruption to increase temperature preference. Moreover, our data indicate that temperature preference may be a more sensitive indicator of sleep disruption than learning and memory.

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Section: Resultsmentioning

confidence: 95%

Section: Methodsmentioning

confidence: 99%

Sleep Deprivation, Sleep Fragmentation and Social Jet Lag increase temperature preference inDrosophila

Roach

Ford

Simhambhatla

et al. 2023

Preprint

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“…Fifty males and 50 females of each fly species were tested and their movements were video taped for 30 minutes. We used the temperature of the region of the channel where an individual fly spent most of its time as a proxy for its preferred temperature 17,18 . To determine possible effects of developmental temperature, D.…”

Section: Temperature Preference Assaymentioning

confidence: 99%

Temperature-dependent modulation of odor-dependent behavior in three drosophilid fly species of differing thermal preference

Baleba

Mahadevan

Knaden

et al. 2023

Preprint

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Rapid and ongoing climate change increases global temperature and impacts both feeding and reproduction in insects. The sense of smell plays an important underlying role in these behaviors in most insect species. Here, we aimed to investigate how changing temperatures affect odor detection and ensuing behavior in three drosophilid flies: Drosophila novamexicana, D. virilis and D. ezoana, species that have adapted to life in desert, global and subarctic climates, respectively. Using a series of thermal preference assays, we confirmed that the three species indeed exhibit distinct temperature preferences. Next, using single sensillum recording technique, we classified olfactory sensory neurons (OSNs) present in basiconic sensilla on the antenna of the three species and thereby identified ligands for each OSN type. In a series of trap assays we proceeded to establish the behavioral valence of the best ligands and chose guaiacol, methyl salicylate and isopropyl benzoate as representatives of a repellent, attractant and neutral odor. Next, we assessed the behavioral valence of these three odors in all three species across a thermal range (10-35 C), with flies reared at 18°C and 25°C. We found that both developmental and experimental temperatures affected the behavioral performance of the flies. Our study thus reveals temperature-dependent changes in odor-guided behavior in drosophilid flies.

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“…In flies, exposure to CO 2 can alter their flying behaviour 23 , hence it is recommended to use ice 22 .…”

Section: Description Of Critical Steps In the Protocolmentioning

confidence: 99%

“…Empty the insects into the funnel that has been placed in the acrylic tube. NOTE: If desired/required: for mosquitoes, use a CO 2 pen to knock out all mosquitoes before placing them in the funnel21 ; for Drosophila, use ice to knock insects down22 .…”

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confidence: 99%

Determining Temperature Preference of Mosquitoes and Other Ectotherms

Haziqah-Rashid

Stobierska²,

Glenn³

et al. 2022

JoVE

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Most insects and other ectotherms have a relatively narrow optimal temperature window, and deviation from their optima can have significant effects on their fitness, as well as other characteristics. Consequently, many such ectotherms seek out their optimal temperature range. Although temperature preferences of mosquitoes and other insects have been well studied, the traditional experimental setup is performed using a temperature gradient on an aluminum surface in a highly enclosed space. In some cases, this equipment restricts many natural behaviors, such as flying, which may be important in preference selection.The objective of this study is to observe insect preference for air temperature by using a two-chamber apparatus with sufficient room for flight. The two chambers consist of independent temperature-controlled incubators, each with a large aperture. The incubators are connected by these apertures using a short acrylic bridge. Inside the incubators are two netted cages, linked via the apertures and bridge, allowing the insects to freely fly between the different conditions. The acrylic bridge also acts as a temperature gradient between the two incubators.Due to the spacious area in the cage and easy construction, this method can be used to study any small ectotherm and/or any manipulation which may alter temperature preference including sensory organ manipulation, diet, gut flora, and endosymbiont presence at biosafety levels 1 or 2 (BSL 1 or 2). Additionally, the apparatus can be used for the study of pathogen infection using further containment (e.g., inside of a biosafety cabinet) at BSL 3.

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Comparative analysis of temperature preference behavior and effects of temperature on daily behavior in 11 Drosophila species

Cited by 21 publications

References 49 publications

Sleep Deprivation, Sleep Fragmentation and Social Jet Lag increase temperature preference inDrosophila

Sleep Deprivation, Sleep Fragmentation and Social Jet Lag increase temperature preference inDrosophila

Temperature-dependent modulation of odor-dependent behavior in three drosophilid fly species of differing thermal preference

Determining Temperature Preference of Mosquitoes and Other Ectotherms

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