Comparative analysis of the expression of metalloproteases and their inhibitors in resected crohnʼs disease and complicated diverticular disease

Altadill, Antonio; Eiró, Noemí; González, Luis O.; Junquera, Sara; González-Quintana, José Manuel; Sánchez, María Rosario; Andicoechea, Alejandro; Saro, Cristina; Rodrigo, Luís

doi:10.1002/ibd.21682

Cited by 35 publications

(29 citation statements)

References 37 publications

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“…Si bien no existen estudios en diverticulitis de intestino delgado, sí los hay en colon donde se demostró la presencia de TNF-α9, SD-1 (familia de sindecanos, reparadores tisulares 12 ) y bFGF (factor de crecimiento fibroblástico básico, reparador de células epiteliales por su capacidad de unirlas a células estromales 13 ) como mediadores inflamatorios comunes en ambas patologías. Sin embargo, se ha demostrado diferencias en estos mediadores en áreas resecadas durante el cuadro agudo, predominando en la EC las metaloproteinasas 2, 9 y 13 versus las tipo 1 y los inhibidores tisulares de metaloproteinasas tipo 1 y 3 en diverticulitis aguda colónica 14 .…”

Section: Discussionunclassified

Diverticulitis aguda de intestino delgado en un paciente con enfermedad de Crohn: Report of one case

et al. 2017

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Section: Discussionunclassified

Diverticulitis aguda de intestino delgado en un paciente con enfermedad de Crohn: Report of one case

et al. 2017

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“…Abnormal expression of MMPs contributes to non-neoplastic pathological conditions, involving acute as well as chronic inflammation and/or tissue degradation, and also contributes to cancer [21][22][23][24][25]. There is evidence supporting the hypothesis that inflammation participates in providing conditions that lead to cancer [26,27], and there are well known associations between inflammatory processes and cancer, such as inflammatory bowel disease and colorectal cancer [28,29], viral hepatitis B and C or alcoholic liver cirrhosis and hepatocarcinoma [30], chronic reflux esophagitis resulting in Barrett's esophagus and esophageal carcinoma [29], cervical infection by human papillomavirus and cervical cancer, prostatitis and prostate cancer, pancreatitis and pancreatic cancer, or gastric infection from Helicobacter pylori and gastric cancer [31,32].…”

Section: Mmps and Timps In Disease And Cancermentioning

confidence: 99%

Clinical Relevance of Matrix Metalloproteases and their Inhibitors in Breast Cancer

2013

J Carcinog Mutagen

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Degradation of the stromal connective tissue and basement membrane components are key elements in tumor invasion and metastasis. Some components, particularly the interstitial collagens, are very resistant to proteolytic attacks and can be degraded by specific proteinases like Matrix Metalloproteinases (MMPs). MMPs can also impact on tumor cell behavior in vivo as a consequence of their ability to cleave growth factors, cell surface receptors, cell adhesion molecules, or chemokines/cytokines, and for stimulating angiogenesis. Different molecular expression profiles of MMPs and their inhibitors (TIMPs) have been associated with the main steps in breast cancer progression, such as creating a potential invasive phenotype in Ductal Carcinoma in situ (DCIS), favoring the hematogenous dissemination, and enabling the metastatic progression across the axillary lymphatic system. These associations have clinical interests, as they can contribute to a better characterization of early breast carcinomas (which differ in their both biological and clinical behavior), evaluate microinvasion in resection specimens of breast tumors, provide a more precise prognostic, and predict the tumoral status of non-sentinel lymph nodes in breast cancer. It is also especially remarkable the evidences indicating that MMPs and TIMPs expression in individual cell populations from tumor stroma, such as mononuclear inflammatory cells (MICs) and fibroblast, clearly impacts on the clinical outcome of breast cancer patients. There are several factors linking inflammation, MMP activity and breast cancer. This knowledge will be useful to develop novel therapies and prevention strategies targeting critical components.

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“…A majority of studies in IBD or animal models have evaluated cathepsins and MMPs at the level of expression of mRNA or protein (9, 15–18) and multiple isoforms of these enzymes are expressed in normal or inflamed intestine (9, 16, 18). The ability to detect and quantify activated cathepsins or MMPs ex vivo or in vivo may provide advantages for detection and localization of intestinal inflammation and evaluation of disease severity in high throughput preclinical studies.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Multiple Enzyme Activatable Near-Infrared Fluorescent Molecular Probes for Detection and Quantification of Inflammation in Murine Colitis Models

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Morgan

et al. 2014

Inflammatory Bowel Diseases

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Background Activatable near-infrared fluorescent (NIRF) probes have been used for ex vivo and in vivo detection of intestinal tumors in animal models. We hypothesized that NIRF probes activatable by cathepsins or MMPs will detect and quantify dextran sulphate sodium (DSS) induced acute colonic inflammation in wild type (WT) mice or chronic colitis in IL-10 null mice ex vivo or in vivo. Methods WT mice given DSS, water controls and IL-10 null mice with chronic colitis were administered probes by retro-orbital injection. FMT2500 LX system imaged fresh and fixed intestine ex vivo and mice in vivo. Inflammation detected by probes was verified by histology and colitis scoring. NIRF signal intensity was quantified using 2D region of interest (ROI) ex vivo or 3D ROI-analysis in vivo. Results Ex vivo, seven probes tested yielded significant higher NIRF signals in colon of DSS treated mice versus controls. A subset of probes was tested in IL-10 null mice and yielded strong ex vivo signals. Ex vivo fluorescence signal with 680 series probes was preserved after formalin fixation. In DSS and IL-10 null models, ex vivo NIRF signal strongly and significantly correlated with colitis scores. In vivo, ProSense680, CatK680FAST and MMPsense680 yielded significantly higher NIRF signals in DSS treated mice than controls but background was high in controls. Conclusion Both cathepsin or MMP-activated NIRF-probes can detect and quantify colonic inflammation ex vivo. ProSense680 yielded the strongest signals in DSS colitis ex vivo and in vivo, but background remains a problem for in vivo quantification of colitis.

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Comparative analysis of the expression of metalloproteases and their inhibitors in resected crohnʼs disease and complicated diverticular disease

Abstract: These results indicate a differential pattern of expression of MMPs and TIMPs in CD and diverticulitis and the necessity to study the potential role of MMP inhibitors as new protective agents in both diseases.

Cited by 35 publications

References 37 publications

Diverticulitis aguda de intestino delgado en un paciente con enfermedad de Crohn: Report of one case

Diverticulitis aguda de intestino delgado en un paciente con enfermedad de Crohn: Report of one case

Clinical Relevance of Matrix Metalloproteases and their Inhibitors in Breast Cancer

Comparison of Multiple Enzyme Activatable Near-Infrared Fluorescent Molecular Probes for Detection and Quantification of Inflammation in Murine Colitis Models

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