Comparative analysis of the genes encoding 23S-5S rRNA intergenic spacer regions of Lactobacillus casei-related strains.

Abstract

In this study, investigations into the 23S-5S rRNA intergenic spacer regions (ISRs) of the

The status of the species Lactobacillus casei (Orla-Jensen 1916) Hansen and Lessel 1971 and Lactobacillus paracasei Collins et al. 1989. Request for an opinion.

“…Moreover, the sequence of the long intergenic spacer region of strain ATCC 393 T has only about 80n2 % similarity with the spacers of the other strains of Lactobacillus paracasei and 72n4 % for the short intergenic spacer region. Finally, the BamHI and PstI ribotypes of strain ATCC 393 T were distinct from the others in the group, whereas strain ATCC 334 shared high levels of similarity with them (Chen et al, 2000). Their results support the request for the taxonomic position of the Lactobacillus casei group proposed by Dellaglio et al (1991).…”

“…Furthermore, recent genotypic evidence (Mori et al, 1997 ;Tynkkynen et al, 1999 ;Chen et al, 2000 ;Felis et al, 2001) strongly supports previous indications (Mills & Lessel, 1973 ;Dellaglio et al, 1975Dellaglio et al, , 1991Dicks et al, 1996) that strains ATCC 393 and ATCC 15820 are members of the same taxon. Even though the valid status of the species Lactobacillus zeae was recognized only in 1996 (Dicks et al, 1996), the misidentified strain ATCC 393 is closely related to the type strain of this species.…”

“…tolerans. (3) On the basis that the species Lactobacillus casei has no type strain (see Request 1), we propose strain ATCC 334 as the neotype, since it has been already proposed (Dellaglio et al, 1991 ;Dicks et al, 1996), supported (Mori et al, 1997 ;Tynkkynen et al, 1999 ;Chen et al, 2000 ;Felis et al, 2001) and used as a reference strain (Yoon et al, 2000 ;Cai et al, 1999 ;Morlon-Guyot et al, 1998) (Rule 19). Furthermore, strain LMG 9191 T may be retrieved as the type strain of the subspecies Lactobacillus casei subsp.…”

Comparative sequence analysis of a recA gene fragment brings new evidence for a change in the taxonomy of the Lactobacillus casei group.

“…Dicks et al (1996) Nour (1998) proposed that 16S-23S and 23S-5S intergenic spacer region sequences might represent a useful alternative to 16S and 23S rDNA for establishing the phylogeny of closely related bacterial species, because of the higher sequence and fragment length polymorphism. Comparative analysis of the 23S-5S rRNA intergenic spacer regions of L. caseirelated strains was performed by Chen et al (2000). Their observations supported the request of Dellaglio et al (1991) to replace strain ATCC 393 T with strain ATCC 334 as the neotype of L. casei subsp.…”

“…The observations on recA K nuc values and phylogenetic inference complement the data on 23S-5S rRNA intergenic spacer regions (Chen et al, 2000), 16S rDNA K nuc values and sequence signatures (Mori et al, 1997), DNA-DNA relatedness (Dellaglio et al, 1975) and numerical analysis of total soluble cell proteins patterns (Dellaglio et al, 1991) in demonstrating that L. casei ATCC 393 T is not representative of the L. casei species.…”