Comparative analysis of the immunostimulatory properties of different adjuvants on the immunogenicity of a prototype parainfluenza virus type 3 subunit vaccine

Ewasyshyn, M.; Caplan, Barry; Bonneau, A.-M.; Scollard, N.; Graham, Stephen M.; Usman, Saheed Opeyemi; Klein, Michel

doi:10.1016/0264-410x(92)90072-r

Cited by 18 publications

(12 citation statements)

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“…Fully glycosylated, affinity-purified PIV-3 HN and F proteins elicited a protective response in cotton rats and hamsters (Ambrose et al, 1991 ;Ewasyshyn et al, 1992), whereas the immunoprotective ability of the deglycosylated antigens has not yet been reported. Thus, the objective of this study was to determine the role of the carbohydrate moieties in the immunoprotective ability of the PIV-3 HN and F proteins.…”

mentioning

confidence: 93%

“…Vero cells were used in the TCIDs0 assay. Viral fluids were harvested and processed using the method outlined by Ewasyshyn et al (1992). The HN and F proteins used in the deglycosylation studies were copurified from detergent-solubilized virus by lentil-lectin affinity chromatography (Ewasyshyn et al, 1992).…”

mentioning

confidence: 99%

“…Viral fluids were harvested and processed using the method outlined by Ewasyshyn et al (1992). The HN and F proteins used in the deglycosylation studies were copurified from detergent-solubilized virus by lentil-lectin affinity chromatography (Ewasyshyn et al, 1992). The protein concentration of the copurified HN and F preparation was determined by a modified Lowry assay (Peterson, 1977).…”

mentioning

confidence: 99%

“…The immunoreactivity of mock-and enzyme-treated proteins was tested by Western blot analysis as previously described (Ewasyshyn et al, 1992) using guinea-pig monospecific anti-HN and anti-F1 peptide antisera. To prepare these antisera, guinea-pigs were immunized with three 50 lag doses of synthetic peptides corresponding to residues 430 to 448 (SKLQLGIIDITDYSDIRIK) and 496 to 522 (LDSQKSRVNPVITYSTSTERVNELAIR) of the HN protein and residues 248 to 274 (VDKYDIYDLLFTESIKVRVIDVDLNDY) and 363 to 390 (CPRTVVTSDIVPRYAFVNGGVVANCITT) of the F1 protein emulsified in Freund's adjuvant.…”

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confidence: 99%

“…Virus titres were determined in both the upper (nasal washes) and lower (lung homogenates) respiratory tracts. Lung homogenates and nasal washes were prepared for viral assay by the procedure outlined by Ewasyshyn et al (1992). As shown in Table 2, there was no significant difference in the ability of mock-and endo F-treated proteins to protect either the upper or lower respiratory tracts of animals against live virus challenge.…”

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confidence: 99%

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Comparative analysis of the immunoprotective abilities of glycosylated and deglycosylated parainfluenza virus type 3 surface glycoproteins

Ewasyshyn¹,

Bonneau

Usman³

et al. 1993

Journal of General Virology

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confidence: 93%

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confidence: 99%

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confidence: 99%

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confidence: 99%

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confidence: 99%

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Comparative analysis of the immunoprotective abilities of glycosylated and deglycosylated parainfluenza virus type 3 surface glycoproteins

Ewasyshyn¹,

Bonneau

Usman³

et al. 1993

Journal of General Virology

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Prospects for a parainfluenza virus vaccine

Ewasyshyn¹,

Cates²,

Jackson

et al. 1997

Pediatric Pulmonology

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Vaccine Design

1997

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Aseries presenting the results of aetlvities sponsored by the NA TO Seienee Committee, whieh aims at the dissemination of advaneed seientlflc and technologleal knowledge, with a vlew to strengthenlng links between seientific communities. The series is publlshed by an International board of publlshers in conjunctlon with the NATO Sclentific Affairs Division A Llfe Sclences B Physlcs All rights reservedNo part 01 thls book may be reproduced, stored in a retrleval syst~m, or transmitted in any lorm or by any means, electronlc, mechanlcal, photocopylng, mlcrolilming. recording, or otherwlse, wlthout written perm Iss Ion lrom the Publlsher ISBN 978-1-4899-0064-7 ISBN 978-1-4899-0062-3 (eBook) PREFACEDuring the last decade or so vaccine development has been facilitated by rapid advances in the molecular and cell biology ofthe immune system. This has laid the foundations of a new generation of vaccines exemplified by subunit vaccines produced through gene cloning and by synthetic peptides mimicking small regions ofproteins on the outer coat ofviruses. However, as subunit and peptide vaccines are only weakly or non-immunogenic, there is areal need for strategies to improve their potency.This book contains the proceedings of the 5th NATO Advanced Studies Institute (ASI), "Vaccine Design: The Role of Cytokine Networks," held at Cape Sounion Beach, Greece, during 24 June-5 July 1996 and deals in depth with the role ofbasic immunology in the regulation of immunity and vaccine design. Special emphasis is given to the use of cytokines in conjuction with vaccines with the aim ofimproving their potency or the use ofvaccines designed to improve cytokine production.

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Comparative analysis of the immunostimulatory properties of different adjuvants on the immunogenicity of a prototype parainfluenza virus type 3 subunit vaccine

Cited by 18 publications

References 27 publications

Comparative analysis of the immunoprotective abilities of glycosylated and deglycosylated parainfluenza virus type 3 surface glycoproteins

Comparative analysis of the immunoprotective abilities of glycosylated and deglycosylated parainfluenza virus type 3 surface glycoproteins

Prospects for a parainfluenza virus vaccine

Vaccine Design

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