Comparative analysis of the intestinal bacterial communities in mud crab <i>Scylla serrata</i> in South India

Apine, Elina; Rai, Praveen; Mani, Madhu K.; Subramanian, Vikram; Karunasagar, Indrani; Godhe, Anna; Turner, Lucy M.

doi:10.1002/mbo3.1179

Cited by 10 publications

(7 citation statements)

References 49 publications

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“…By comparison, salinity, crab mass and carapace width, geographical location as well as whether they were farmed, or wild-caught crabs did not have a significant impact on gut microbiome composition. In addition, the results indicate that farming does not significantly alter the composition of the gut microbiome when compared to wild-caught crabs [82].…”

Section: Oxidative Stress Modification Through Nutrients and Microbiotamentioning

confidence: 84%

Role of Food Antioxidants in Modulating Gut Microbial Communities: Novel Understandings in Intestinal Oxidative Stress Damage and Their Impact on Host Health

et al. 2021

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Dietary components have an important role on the structure and function of host gut microbial communities. Even though, various dietary components, such as carbohydrates, fats, proteins, fibers, and vitamins, have been studied in depth for their effect on gut microbiomes, little attention has been paid regarding the impact of several food antioxidants on the gut microbiome. The long-term exposure to reactive oxygen species (ROS) can cause microbial dysbiosis which leads to numerous intestinal diseases such as microbiota dysbiosis, intestinal injury, colorectal cancers, enteric infections, and inflammatory bowel diseases. Recently, it has been shown that the food derived antioxidant compounds might protect the host from intestinal oxidative stress via modulating the composition of beneficial microbial species in the gut. The present review summarizes the impact of food antioxidants including antioxidant vitamins, dietary polyphenols, carotenoids, and bioactive peptides on the structure as well as function of host gut microbial communities. Several in vitro, animal model, and clinical studies indicates that food antioxidants might modify the host gut microbial communities and their health status. However, still further clarification is needed as to whether changes in certain microbial species caused by food additives may lead to changes in metabolism and immune function.

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Section: Oxidative Stress Modification Through Nutrients and Microbiotamentioning

confidence: 84%

Role of Food Antioxidants in Modulating Gut Microbial Communities: Novel Understandings in Intestinal Oxidative Stress Damage and Their Impact on Host Health

et al. 2021

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“…However, the functional role and importance of symbiotic microbes in nutrition have seldom been systematically investigated in any crab. Metagenomes for the gut symbiont were only sequenced for a few species with commercial importance (e.g., Chinese mitten crab Eriocheir sinensis [28,29]; mud crab Scylla spp [30,31]). The gut bacterial communities were characterized on three mangrove sesarmid crabs using 16S amplicon sequencing and microbes with capacity of cellulose degradation and nitrogen fixation were found [32].…”

Section: Introductionmentioning

confidence: 99%

Metagenomic analysis of gut microbiome illuminates the mechanisms and evolution of lignocellulose degradation in mangrove herbivorous crabs

Hui,

Lo,

Wong

et al. 2024

BMC Microbiol

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Background Sesarmid crabs dominate mangrove habitats as the major primary consumers, which facilitates the trophic link and nutrient recycling in the ecosystem. Therefore, the adaptations and mechanisms of sesarmid crabs to herbivory are not only crucial to terrestrialization and its evolutionary success, but also to the healthy functioning of mangrove ecosystems. Although endogenous cellulase expressions were reported in crabs, it remains unknown if endogenous enzymes alone can complete the whole lignocellulolytic pathway, or if they also depend on the contribution from the intestinal microbiome. We attempt to investigate the role of gut symbiotic microbes of mangrove-feeding sesarmid crabs in plant digestion using a comparative metagenomic approach. Results Metagenomics analyses on 43 crab gut samples from 23 species of mangrove crabs with different dietary preferences revealed a wide coverage of 127 CAZy families and nine KOs targeting lignocellulose and their derivatives in all species analyzed, including predominantly carnivorous species, suggesting the crab gut microbiomes have lignocellulolytic capacity regardless of dietary preference. Microbial cellulase, hemicellulase and pectinase genes in herbivorous and detritivorous crabs were differentially more abundant when compared to omnivorous and carnivorous crabs, indicating the importance of gut symbionts in lignocellulose degradation and the enrichment of lignocellulolytic microbes in response to diet with higher lignocellulose content. Herbivorous and detritivorous crabs showed highly similar CAZyme composition despite dissimilarities in taxonomic profiles observed in both groups, suggesting a stronger selection force on gut microbiota by functional capacity than by taxonomy. The gut microbiota in herbivorous sesarmid crabs were also enriched with nitrogen reduction and fixation genes, implying possible roles of gut microbiota in supplementing nitrogen that is deficient in plant diet. Conclusions Endosymbiotic microbes play an important role in lignocellulose degradation in most crab species. Their abundance is strongly correlated with dietary preference, and they are highly enriched in herbivorous sesarmids, thus enhancing their capacity in digesting mangrove leaves. Dietary preference is a stronger driver in determining the microbial CAZyme composition and taxonomic profile in the crab microbiome, resulting in functional redundancy of endosymbiotic microbes. Our results showed that crabs implement a mixed mode of digestion utilizing both endogenous and microbial enzymes in lignocellulose degradation, as observed in most of the more advanced herbivorous invertebrates.

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“…Bacteria belonging to the Firmicutes are known to produce a wide range of digestive enzymes, facilitating host digestion and nutrient absorption ( Hudson and Egan, 2022 ). Proteobacteria dominate the gut microbiota of aquatic crustaceans ( Ding et al, 2017 ; Apine et al, 2021 ) and have the ability to synthesize essential micronutrients like vitamin K2. They also regulate gut immunity by enhancing immune cell production and producing beneficial short-chain fatty acids, which contribute to energy metabolism and intestinal health ( Saqib et al, 2023 ).…”

Section: Discussionmentioning

confidence: 99%

The shared microbiome in mud crab (Scylla paramamosain) of Sanmen Bay, China: core gut microbiome

Jiang,

Niu,

Qin

et al. 2023

Front. Microbiol.

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IntroductionThe mud crab, Scylla paramamosain, holds great commercial significance as a marine crustacean widely cultivated in the Indo-Pacific region. Understanding the core gut microbiota of aquatic animals is crucial for their overall health and growth, yet the core gut microbiota of mud crab remains poorly characterized.MethodsIn this study, we gathered gut samples from mud crabs across five locations within Sanmen Bay, China. Through the utilization of high-throughput sequencing, we delved into the composition of the gut microbial community and identified the core gut microbiome of mud crab.ResultsOur results demonstrate that the gut microbial diversity of mud crab did not exhibit significant variation among the five sampling sites, although there were some differences in community richness. At the phylum level, we identified 35 representative phyla, with Firmicutes, Proteobacteria, Bacteroidota, and Campilobacterota as the dominant phyla. Among the 815 representative genera, we discovered 19 core genera, which accounted for 65.45% of the total sequences. These core genera were distributed across 6 phyla, and among them, Photobacterium exhibited the highest average relative abundance.DiscussionPhotobacterium has probiotic activity and may play a crucial role in enhancing the immune response of the host and maintaining the diversity of the gut microbiota. Moreover, we observed a positive correlation between the relative abundance of core genera and the stability of the gut microbial community. Furthermore, our findings revealed distinct differences in gut microbial composition and specific taxa between the sexes of mud crab. These differences subsequently influenced the functionality of the gut microbial community. Overall, our investigation sheds light on the core gut microbiota of mud crab, emphasizing the importance of core gut microbial communities in maintaining the health and growth of these commercially significant marine crustaceans.

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Comparative analysis of the intestinal bacterial communities in mud crab Scylla serrata in South India

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 10 publications

References 49 publications

Role of Food Antioxidants in Modulating Gut Microbial Communities: Novel Understandings in Intestinal Oxidative Stress Damage and Their Impact on Host Health

Role of Food Antioxidants in Modulating Gut Microbial Communities: Novel Understandings in Intestinal Oxidative Stress Damage and Their Impact on Host Health

Metagenomic analysis of gut microbiome illuminates the mechanisms and evolution of lignocellulose degradation in mangrove herbivorous crabs

The shared microbiome in mud crab (Scylla paramamosain) of Sanmen Bay, China: core gut microbiome

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