Comparative Analysis of the Mechanism of Resistance to Silver Nanoparticles and the Biocide 2,2-Dibromo-3-Nitrilopropionamide

Alhajjar, Rehab K.; Roche, Kayley M.; Techtmann, Stephen M.

doi:10.1128/aac.02031-21

Cited by 4 publications

(4 citation statements)

References 63 publications

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“…However, despite their known properties, the molecular mechanisms behind their antimicrobial activity are not fully determined or understood. In an attempt to answer this question, in the last decade, a vast number of studies have been conducted [ 205 , 206 , 207 , 208 , 209 , 210 , 211 , 212 , 213 , 214 , 215 , 216 , 217 , 218 , 219 , 220 , 221 , 222 , 223 , 224 , 225 , 226 , 227 , 228 , 229 , 230 , 231 , 232 , 233 , 234 , 235 , 236 , 237 , 238 , 239 , 240 , 241 , 242 ]. In these studies, mostly AgNPs (72% of studies) but also other metal and metal oxide NPs, such as zinc oxide nanoparticles (ZnONPs) (10%) and copper nanoparticles (CuNPs) (5%)—and also gold, iron oxide, magnesium oxide, manganese and titanium nanoparticles (AuNPs, FeONPs, MgONPs, Mn and TiO 2 NPs) (3% each)—were used against Gram-negative (77%) and Gram-positive (38%) bacteria and against fungi (5%).…”

Section: Antimicrobial Activity Of Metal Nanoparticlesmentioning

confidence: 99%

“…Despite some disagreement [ 205 , 206 ], most studies determined that the antimicrobial mechanism of MNPs involves, at some point, the formation of reactive oxygen species (ROS) [ 207 , 208 , 209 , 210 , 211 , 212 , 213 , 214 , 215 , 216 , 217 , 218 , 219 , 220 , 221 , 222 , 223 , 224 ] and intracellular content leakage due to cell membrane disruption [ 208 , 209 , 210 , 211 , 212 , 213 , 214 , 215 , 216 , 217 , 218 , 219 , 220 , 221 , 224 , 225 , 226 , 227 , 228 , 229 , 230 , 231 , 232 ]. However, the reasons behind these occurrences and the order in which they occur differ across studies.…”

Section: Antimicrobial Activity Of Metal Nanoparticlesmentioning

confidence: 99%

“…However, the reasons behind these occurrences and the order in which they occur differ across studies. Many also agree that metallic ions (M x ) availability inside cells is important [ 206 , 207 , 209 , 211 , 213 , 215 , 217 , 223 , 228 , 229 , 233 , 234 , 235 , 236 , 237 ], with some showing that M x extracellular sequestration by extracellular polymeric substance (EPS) decreases the antimicrobial activity of MNPs [ 225 , 236 ]. This interaction with EPS might also hinder biofilm formation and be the reason behind some MNPs antibiofilm properties [ 208 , 224 ].…”

Section: Antimicrobial Activity Of Metal Nanoparticlesmentioning

confidence: 99%

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Biosynthesis of Metal and Metal Oxide Nanoparticles Using Microbial Cultures: Mechanisms, Antimicrobial Activity and Applications to Cultural Heritage

et al. 2023

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Nanoparticles (1 to 100 nm) have unique physical and chemical properties, which makes them suitable for application in a vast range of scientific and technological fields. In particular, metal nanoparticle (MNPs) research has been showing promising antimicrobial activities, paving the way for new applications. However, despite some research into their antimicrobial potential, the antimicrobial mechanisms are still not well determined. Nanoparticles’ biosynthesis, using plant extracts or microorganisms, has shown promising results as green alternatives to chemical synthesis; however, the knowledge regarding the mechanisms behind it is neither abundant nor consensual. In this review, findings from studies on the antimicrobial and biosynthesis mechanisms of MNPs were compiled and evidence-based mechanisms proposed. The first revealed the importance of enzymatic disturbance by internalized metal ions, while the second illustrated the role of reducing and negatively charged molecules. Additionally, the main results from recent studies (2018–2022) on the biosynthesis of MNPs using microorganisms were summarized and analyzed, evidencing a prevalence of research on silver nanoparticles synthesized using bacteria aiming toward testing their antimicrobial potential. Finally, a synopsis of studies on MNPs applied to cultural heritage materials showed potential for their future use in preservation.

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Section: Antimicrobial Activity Of Metal Nanoparticlesmentioning

confidence: 99%

Section: Antimicrobial Activity Of Metal Nanoparticlesmentioning

confidence: 99%

Section: Antimicrobial Activity Of Metal Nanoparticlesmentioning

confidence: 99%

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Biosynthesis of Metal and Metal Oxide Nanoparticles Using Microbial Cultures: Mechanisms, Antimicrobial Activity and Applications to Cultural Heritage

et al. 2023

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“…These problems result in a higher required biocide concentration compared to combating suspension bacteria (Saleh et al, 2021 ). Due to high resistance and high cytotoxicity (Alhajjar et al, 2022 ; McLaughlin et al, 2021 ), the application of biocides is limited, and the development of new antimicrobial and antibiofilm candidates is crucial. In recent years, antimicrobial peptides (AMPs) have emerged as a new treatment strategy (Luo et al, 2023 ).…”

Section: Introductionmentioning

confidence: 99%

Antibiofilm assay for antimicrobial peptides combating the sulfate‐reducing bacteria Desulfovibrio vulgaris

Stillger,

Viau,

Holtmann

et al. 2023

MicrobiologyOpen

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In medical, environmental, and industrial processes, the accumulation of bacteria in biofilms can disrupt many processes. Antimicrobial peptides (AMPs) are receiving increasing attention in the development of new substances to avoid or reduce biofilm formation. There is a lack of parallel testing of the effect against biofilms in this area, as well as in the testing of other antibiofilm agents. In this paper, a high‐throughput screening was developed for the analysis of the antibiofilm activity of AMPs, differentiated into inhibition and removal of a biofilm. The sulfate‐reducing bacterium Desulfovibrio vulgaris was used as a model organism. D. vulgaris represents an undesirable bacterium, which is considered one of the major triggers of microbiologically influenced corrosion. The application of a 96‐well plate and steel rivets as a growth surface realizes real‐life conditions and at the same time establishes a flexible, simple, fast, and cost‐effective assay. All peptides tested in this study demonstrated antibiofilm activity, although these peptides should be individually selected depending on the addressed aim. For biofilm inhibition, the peptide DASamP1 is the most suitable, with a sustained effect for up to 21 days. The preferred peptides for biofilm removal are S6L3‐33, in regard to bacteria reduction, and Bactenecin, regarding total biomass reduction.

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Antibacterial activity against pathogenic Vibrio and cytotoxicity on human hepatocyte of nano-silver prepared by polysaccharide-protein complexes

He,

Wang,

Jian

2024

Front. Microbiol.

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Silver nanoparticles (AgNPs) are potential antibacterial agents against pathogenic Vibrio bacteria in the field of public health, yet their widespread use is limited by dispersibility and biocompatibility. In a previous study, highly dispersible AgNPs were fabricated using a polysaccharide–protein complex (PSP) obtained from the viscera of Haliotis discus. In this study, the antibacterial activity of PSP-AgNPs against pathogenic Vibrio and its cytotoxicity for human hepatocytes (LO2) was evaluated. At dosages of 3.125–25.0 μg/mL, PSP-AgNPs demonstrated excellent antibacterial activity against several pathogenic Vibrio strains (such as V. fluvialis, V. mimicus, V. hollisae, V. vulnificus, and V. furnissii), and no cytotoxicity on LO2 cells. This was evidenced by cellular viability, reactive oxygen species, and antioxidase activities. However, severe cytotoxicity was observed at a PSP-AgNPs concentration of 50.0 μg/mL. Furthermore, intracellular oxidative stress was the predominant mechanism of toxicity induced by PSP-AgNPs. Overall, PSP-AgNPs are highly biocompatible in the range of effective antibacterial dosages, identifying them as promising bactericide candidates in the field of public health.

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Comparative Analysis of the Mechanism of Resistance to Silver Nanoparticles and the Biocide 2,2-Dibromo-3-Nitrilopropionamide

Abstract: Antimicrobials such as nanoparticles and biocides are used to control microbial growth. We used Escherichia coli to study the process of acquired resistance to silver nanoparticles (Ag-NP) and the industrial biocide DBNPA when grown in sub-MICs.

Cited by 4 publications

References 63 publications

Biosynthesis of Metal and Metal Oxide Nanoparticles Using Microbial Cultures: Mechanisms, Antimicrobial Activity and Applications to Cultural Heritage

Biosynthesis of Metal and Metal Oxide Nanoparticles Using Microbial Cultures: Mechanisms, Antimicrobial Activity and Applications to Cultural Heritage

Antibiofilm assay for antimicrobial peptides combating the sulfate‐reducing bacteria Desulfovibrio vulgaris

Antibacterial activity against pathogenic Vibrio and cytotoxicity on human hepatocyte of nano-silver prepared by polysaccharide-protein complexes

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