Comparative analysis of the oral microbiome of burning mouth syndrome patients

Abstract

Burning mouth syndrome (BMS) is a chronic pain condition accompanied by unpleasant burning sensations of the oral mucosa. While multiple factors were proposed for the etiology, evidence suggested a neuropathic pain origin while others suspected the use of antibiotics as the underlying cause. Interestingly, several reports demonstrated the intimate interaction of the nervous system and the microbiome. The current study aims to elucidate the correlation of the oral microbiome with the pathophysiology of the primary BMS. Microbiome samples obtained from the unstimulated whole saliva of 19 primary BMS patients and 22 healthy controls were sequenced and analyzed of the V3-V4 region of 16S rRNA gene. There was a distinct difference in the microbial composition between the BMS and the control groups at all taxonomic levels. Alpha diversity indexes of the oral microbiome were significantly lower in the BMS group. The samples were readily distinguished by multidimensional scaling analysis and linear discriminant analysis effect size. Streptococcus , Rothia , Bergeyella , and Granulicatella genus were dominant in the BMS group, while Prevotella , Haemophilus , Fusobacterium , Campylobacter , and Allorevotella genus were more abundant in the healthy group. Distinct microbiome signatures of BMS patients suggested a diagnostic value and a potential role in the pathogenesis of BMS.

Regulation of Pain Perception by Microbiota in Parkinson Disease

“…For instance, LPS derived from the odontogenic pathobiont Porphyromonas gingivalis can elicit dose-dependent calcium influx and inward currents, sensitizes TRPV1 to capsaicin, and stimulates the release of calcitonin gene-related peptide in trigeminal ganglia (Diogenes et al, 2011) correlated to the abundance of some bacterial taxa, suggesting a potential causal relationship (Fourie et al, 2016). Another study carried out with burning mouth syndrome patients found similar results, concluding that a higher abundance of Streptococcus, Granulicatella, Bergeyella, and Rothia genus in the oral microbiome was associated with this chronic pain condition (Lee et al, 2022). Nonetheless, further research is necessary to decipher the underlying mechanisms involved in the role of these oral bacteria in chronic pain.…”

Alterations and correlations in dental plaque microbial communities and metabolome characteristics in patients with caries, periodontitis, and comorbid diseases

“…Due to the longer amplicon size of the V1-V3 region compared to V3-V4, the merge rate of the paired-end reads is higher in the V3-V4region than in the V1-V3region [18]. And many recent studies reported that V3-V4 regions could be used for oral microbiota studies [19][20][21].…”

Characterization of the unique oral microbiome of children with Down syndrome

“…Qiime2 was used for the diversity analysis and taxonomic classification, while the Naïve Bayes classifier (Greengenes13_8 99% OTU full-length sequence) was employed for only taxonomic classification. The phylum, genus, and species were extracted from taxa with relative abundance > 1% of the total in the Down syndrome or non-Down syndrome groups, according to the method previously described 28 . All findings related to bacterial phyla, genera, and species obtained from microbiome analysis were validated and polished using the eHOMD (Human Oral Microbiome D) and LPSN databases.…”