Comparative analysis of the protein profile from biofortified cultivars of quality protein maize and conventional maize by gel-based and gel-free proteomic approaches

Deletion of AA9 Lytic Polysaccharide Monooxygenases Impacts A. nidulans Secretome and Growth on Lignocellulose

“…The concentrated secretomes (20 μg) of the mutants carrying single deletions of An LPMO9F, An LPMO9G and An CDH1 (cultivated in Avicel for 24 h) were prepared from in-gel tryptic digestion, as described above. Digested peptides were separated using RP-nanoUPLC (nanoAcquity, Waters) on a C18 column coupled to a Q-TOF Premier mass spectrometer (Waters) with a nanoelectrospray ion source ( 89 ) and data analysis was performed as previously described ( 5 ).…”