Comparative analysis of the UDP-glycosyltransferase multigene family in insects

Ahn, Seung‐Joon; Vogel, Heiko; Heckel, David G.

doi:10.1016/j.ibmb.2011.11.006

Cited by 211 publications

(282 citation statements)

References 69 publications

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“…S2), and 110 genes were common in the lists of up-regulated genes from the DESeq and edgeR analyses. Remarkably, among these 31 genes (Table 1), 12 xenobioticmetabolizing genes, including seven genes encoding phase I enzymes, four genes encoding phase II enzymes (three of which comprise one-quarter of all uridine-diphosphate-glucosyl transferases in the honey bee genome) (20), and two genes encoding phase III enzymes, were up-regulated by p-coumaric acid. In addition to CYP9Q3, known to metabolize pesticides (16), four CYP6AS enzymes known to metabolize honey flavonoids (10) and CYP6BD1 were induced 1.9-to 3.11-fold.…”

Section: Resultsmentioning

confidence: 99%

Honey constituents up-regulate detoxification and immunity genes in the western honey bee Apis mellifera

Mao¹,

Schuler

Berenbaum³

2013

Proc. Natl. Acad. Sci. U.S.A.

266

236

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As a managed pollinator, the honey bee Apis mellifera is critical to the American agricultural enterprise. Recent colony losses have thus raised concerns; possible explanations for bee decline include nutritional deficiencies and exposures to pesticides and pathogens. We determined that constituents found in honey, including p- coumaric acid, pinocembrin, and pinobanksin 5-methyl ether, specifically induce detoxification genes. These inducers are primarily found not in nectar but in pollen in the case of p- coumaric acid (a monomer of sporopollenin, the principal constituent of pollen cell walls) and propolis, a resinous material gathered and processed by bees to line wax cells. RNA-seq analysis (massively parallel RNA sequencing) revealed that p- coumaric acid specifically up-regulates all classes of detoxification genes as well as select antimicrobial peptide genes. This up-regulation has functional significance in that that adding p- coumaric acid to a diet of sucrose increases midgut metabolism of coumaphos, a widely used in-hive acaricide, by ∼60%. As a major component of pollen grains, p- coumaric acid is ubiquitous in the natural diet of honey bees and may function as a nutraceutical regulating immune and detoxification processes. The widespread apicultural use of honey substitutes, including high-fructose corn syrup, may thus compromise the ability of honey bees to cope with pesticides and pathogens and contribute to colony losses.

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Section: Resultsmentioning

confidence: 99%

Honey constituents up-regulate detoxification and immunity genes in the western honey bee Apis mellifera

Mao¹,

Schuler

Berenbaum³

2013

Proc. Natl. Acad. Sci. U.S.A.

266

236

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“…Other gene families may be more relevant in some cases, for example the UDPglucuronosyltransferases (UGTs) and ABC transporters in relation to plant secondary metabolites [45,46] and various digestive hydrolases in relation to their primary metabolites [16,47]. We also acknowledge that simple counts of genes in the various families will mean that members of the families that have nothing to do with detoxification are given equal weighting to specific genes and clades of genes that are likely to be important, such as the CYP6 and CYP4 P450s, the Clade 1 CCEs, and the Delta and Epsilon GSTs [5,13,48].…”

Section: Discussionmentioning

confidence: 99%

Are feeding preferences and insecticide resistance associated with the size of detoxifying enzyme families in insect herbivores?

Rane

Walsh

Pearce

et al. 2016

Current Opinion in Insect Science

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“…This gene is found in many viruses belonging to the two baculovirus genera that infect Lepidoptera, Alphabaculovirus and Betabaculovirus (O’Reilly 1995, 1997; Ahn et al 2012). UDP-glycosyltransferases (UGTs) catalyze the conjugation of a sugar donated by a UDP-glycoside to a lipophilic molecule; baculovirus EGT inactivates host ecdysosteroid hormones by glycosulating them (O’Reilly 1995).…”

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confidence: 99%

“…In their survey of putative cases of HGT from hosts to baculoviruses, Hughes and Friedman (2003) were unable to determine whether baculovirus EGT arose by HGT from their insect hosts because of the lack of sequences from insects of the order Lepidoptera, the hosts of all baculoviruses known to encode EGTs. More recently, numerous sequences of UGTs from Lepidoptera have become available, including those from complete genomes of the silkworm moth Bombyx mori and the monarch butterfly Danaus plexippus (Ahn et al 2012). Using this larger database, Ahn et al (2012) noted that baculovirus EGT sequences showed greatest similarity to a Lepidoptera-specific family of UGTs, which they designated UGT33.…”

mentioning

confidence: 99%

“…More recently, numerous sequences of UGTs from Lepidoptera have become available, including those from complete genomes of the silkworm moth Bombyx mori and the monarch butterfly Danaus plexippus (Ahn et al 2012). Using this larger database, Ahn et al (2012) noted that baculovirus EGT sequences showed greatest similarity to a Lepidoptera-specific family of UGTs, which they designated UGT33. However, those authors did not comment on the evolutionary origin of baculovirus EGT.…”

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Origin of ecdysosteroid UDP-glycosyltransferases of baculoviruses through horizontal gene transfer from Lepidoptera

Hughes

2013

Coevolution

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Baculoviruses infecting Lepidoptera (butterflies and moths) encodes an enzyme known as ecdysosteroid UDP-glycosyltransferase (EGT), which inactivates insect host ecdysosteroid hormones, thereby preventing molt and pupation and permitting a build-up of the viral population within the host. Baculovirus EGT shows evidence of homology to insect UDP-glycosyltransferases, and a phylogenetic analysis supported the closest relative of baculovirus EGT are the UGT33 and UGT34 families of lepidopteran UDP-glycosyltransferases. The phylogenetic analysis thus supported that baculovirus EGT arose by horizontal gene transfer of a UDP-glycosyltransferase from a lepidopteran host, an event that occurred 70 million years ago at the earliest but possibly much more recently. Three amino acid replacements unique to baculovirus EGTs and conserved in all available baculovirus sequences were identified in the N-terminal region of the molecule. Because of their conservation, these amino acids are candidates for playing an important functional role in baculovirus EGT function.

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Comparative analysis of the UDP-glycosyltransferase multigene family in insects

Cited by 211 publications

References 69 publications

Honey constituents up-regulate detoxification and immunity genes in the western honey bee Apis mellifera

Honey constituents up-regulate detoxification and immunity genes in the western honey bee Apis mellifera

Are feeding preferences and insecticide resistance associated with the size of detoxifying enzyme families in insect herbivores?

Origin of ecdysosteroid UDP-glycosyltransferases of baculoviruses through horizontal gene transfer from Lepidoptera

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