Comparative Analysis of Three Bradyrhizobium diazoefficiens Genomes Show Specific Mutations Acquired during Selection for a Higher Motility Phenotype and Adaption to Laboratory Conditions

Lozano, Mauricio Javier; Redondo‐Nieto, Miguel; Garrido‐Sanz, Daniel; Mongiardini, Elías J.; Quelas, Juan Ignacio; Mengucci, Florencia; Dardis, Carolina; Lodeiro, Aníbal Roberto; Althabegoiti, María Julia

doi:10.1128/spectrum.00569-21

Cited by 2 publications

(1 citation statement)

References 47 publications

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“… 15 According to comparative genomic analysis research, nod genes and nif factors are species and host specific. 16 , 17 Although legumes of the genus Bradyrhizobium are known to be capable of forming symbiotic nodules, the genetic repertoire for nitrogen/carbon fixation is still under investigation. Additionally, studies have shown that nod and nif factors are typically arranged as gene clusters that govern nodulation and nitrogen fixation.…”

Section: Introductionmentioning

confidence: 99%

Comparative genomics analysis reveals genetic characteristics and nitrogen fixation profile of Bradyrhizobium

Zhong,

Hu,

et al. 2024

iScience

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Section: Introductionmentioning

confidence: 99%

Comparative genomics analysis reveals genetic characteristics and nitrogen fixation profile of Bradyrhizobium

Zhong,

Hu,

et al. 2024

iScience

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Ribulose 1,5-Bisphosphate Carboxylase/Oxygenase Is Required in Bradyrhizobium diazoefficiens for Efficient Soybean Root Colonization and Competition for Nodulation

Balda,

Cogo,

Falduti

et al. 2024

Plants

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The Hyphomicrobiales (Rhizobiales) order contains soil bacteria with an irregular distribution of the Calvin–Benson–Bassham cycle (CBB). Key enzymes in the CBB cycle are ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO), whose large and small subunits are encoded in cbbL and cbbS, and phosphoribulokinase (PRK), encoded by cbbP. These genes are often found in cbb operons, regulated by the LysR-type regulator CbbR. In Bradyrhizobium, pertaining to this order and bearing photosynthetic and non-photosynthetic species, the number of cbbL and cbbS copies varies, for example: zero in B. manausense, one in B. diazoefficiens, two in B. japonicum, and three in Bradyrhizobium sp. BTAi. Few studies addressed the role of CBB in Bradyrhizobium spp. symbiosis with leguminous plants. To investigate the horizontal transfer of the cbb operon among Hyphomicrobiales, we compared phylogenetic trees for concatenated cbbL-cbbP-cbbR and housekeeping genes (atpD-gyrB-recA-rpoB-rpoD). The distribution was consistent, indicating no horizontal transfer of the cbb operon in Hyphomicrobiales. We constructed a ΔcbbLS mutant in B. diazoefficiens, which lost most of the coding sequence of cbbL and has a frameshift creating a stop codon at the N-terminus of cbbS. This mutant nodulated normally but had reduced competitiveness for nodulation and long-term adhesion to soybean (Glycine max (L.) Merr.) roots, indicating a CBB requirement for colonizing soybean rhizosphere.

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Comparative Analysis of Three Bradyrhizobium diazoefficiens Genomes Show Specific Mutations Acquired during Selection for a Higher Motility Phenotype and Adaption to Laboratory Conditions

Abstract: The genetic and genomic changes that occur under laboratory conditions in Bradyrhizobium diazoefficiens genomes remain poorly studied. Only a few genome sequences of this important nitrogen-fixing species are available, and there are no genome-wide comparative analyses of related strains.

Cited by 2 publications

References 47 publications

Comparative genomics analysis reveals genetic characteristics and nitrogen fixation profile of Bradyrhizobium

Comparative genomics analysis reveals genetic characteristics and nitrogen fixation profile of Bradyrhizobium

Ribulose 1,5-Bisphosphate Carboxylase/Oxygenase Is Required in Bradyrhizobium diazoefficiens for Efficient Soybean Root Colonization and Competition for Nodulation

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