Comparative analysis of tumor control gene and microRNA expression profiles in tumor and adjacent tissues in patients with colorectal cancer

Sluzhev, M. I.; Zaraiski, Mikhail; Семиглазов, Владислав; Semiglazova, T. Yu.; Tkachenko, Elena; Кондратьев, С. В.; Brish, N. A.; Alekseeva, Yu. V.; Petrik, Iu. V.; Sidorova, Aleksandra

doi:10.21294/1814-4861-2022-21-2-55-64

Cited by 1 publication

(1 citation statement)

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“…Macroscopically unchanged (conditionally normal) lung tissue was taken at a distance of at least 5 cm from the tumor margin. This method of selecting control tissue is widely used in scientific work and makes it possible to identify genes with altered expression profile, which are associated with carcinogenesis [13][14][15]. A total of 40 pairs of samples were collected from smoking patients with SCC and lung adenocarcinoma (LAC) and 30 pairs of samples from never-smoking patients with LAC.…”

Section: Tissue Samplesmentioning

confidence: 99%

Expression of AhR-regulated miRNAs in non-small cell lung cancer in smokers and never smokers

Kalinina,

Kononchuk,

Valembakhov

et al. 2024

BIOMED KHIM

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Smoking is a risk factor for non-small cell lung cancer (NSCLC). The most common subtypes of NSCLC are lung adenocarcinoma (LAC) and squamous cell carcinoma (SCC). The cigarette smoke contains aryl hydrocarbon receptor (AhR) ligands, such as benzo(a)pyrene (BaP). By activating the AhR, BaP can change the expression of many genes, including miRNA-encoding genes. In this study, we have evaluated the expression of few miRNAs potentially regulated by AhR (miR-21, -342, -93, -181a, -146a), as well as CYP1A1, a known AhR target gene, in lung tumor samples from smoking (n=40) and non-smoking (n=30) patients with LAC and from smoking patients with SCC (n=40). We have also collected macroscopically normal lung tissue >5 cm from the tumor margin. We compared the obtained data on the miRNA expression in tumors with data from The Cancer Genome Atlas (TCGA). We found that in 76.7% of non-smoking LAC patients, CYP1A1 mRNA was not detected in tumor and normal lung tissues, while in smoking patients, CYP1A1 expression was detected in tumors in almost half of the cases (47.5% for SCC and 42.5% for LAC). The expression profile of AhR-regulated miRNAs differed between LAC and SCC and depended on the smoking status. In LAC patients, the expression of oncogenic miRNA-21 and miRNA-93 in tumors was higher than in normal lung tissue from the same patients. However, in SCC patients from our sample, the levels of these miRNAs in tumor and non-transformed lung tissue did not differ significantly. The results of our studies and TCGA data indicate that the expression levels of miRNA-181a and miRNA-146a in LAC are associated with smoking: expression of these miRNAs was significantly lower in tumors of smokers. It is possible that their expression is regulated by AhR and AhRR (AhR repressor), and inhibition of AhR by AhRR leads to a decrease in miRNA expression in tumors of smoking patients. Overall, these results confirm that smoking has an effect on the miRNA expression profile. This should be taken into account when searching for new diagnostic and therapeutic targets for NSCLC.

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Section: Tissue Samplesmentioning

confidence: 99%