Comparative analysis of two-component signal transduction systems of Bacillus cereus, Bacillus thuringiensis and Bacillus anthracis

Been, M.W.H.J. de; Francke, Christof; Moezelaar, Roy; Abee, Tjakko; Siezen, Roland J.

doi:10.1099/mic.0.29137-0

Cited by 53 publications

(68 citation statements)

References 89 publications

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“…Recovered sequences were further scrutinized according to the following criteria: (i) the HATPase domain had to be located in the C-terminus (last 2/3) of the encoded protein and (ii) a putative H-box had to precede the HATPase domain. Detection of HK-RR gene pairs and 'orphan' HK and RR genes was similar to that described previously [11].…”

Section: Identification Of Hks and Rrssupporting

confidence: 64%

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Comparative Genomic Analysis of Two-Component Signal Transduction Systems in Probiotic Lactobacillus casei

Peng

Chen³

et al. 2014

Indian J Microbiol

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Lactobacillus casei has traditionally been recognized as a probiotic, thus needing to survive the industrial production processes and transit through the gastrointestinal tract before providing benefit to human health. The two-component signal transduction system (TCS) plays important roles in sensing and reacting to environmental changes, which consists of a histidine kinase (HK) and a response regulator (RR). In this study we identified HKs and RRs of six sequenced L. casei strains. Ortholog analysis revealed 15 TCS clusters (HK-RR pairs), one orphan HKs and three orphan RRs, of which 12 TCS clusters were common to all six strains, three were absent in one strain. Further classification of the predicted HKs and RRs revealed interesting aspects of their putative functions. Some TCS clusters are involved with the response under the stress of the bile salts, acid, or oxidative, which contribute to survive the difficult journey through the human gastrointestinal tract. Computational predictions of 15 TCSs were verified by PCR experiments. This genomic level study of TCSs should provide valuable insights into the conservation and divergence of TCS proteins in the L. casei strains.

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Section: Identification Of Hks and Rrssupporting

confidence: 64%

“…Zhang was similar to that described previously [11]. Briefly, the HMM profile (Accession numbers PF00512) was found in Pfam database that targets the HisKA family of HKs, which was used to recognize the HKs in the L. casei genomes.…”

Section: Identification Of Hks and Rrsmentioning

confidence: 99%

Comparative Genomic Analysis of Two-Component Signal Transduction Systems in Probiotic Lactobacillus casei

Peng

Chen³

et al. 2014

Indian J Microbiol

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“…Among the genes identified in this study, one (BC5440) was found to encode a protein of a two-component regulatory system that may function to sense and perceive the environment more precisely and respond to it. The BC5440 gene product does not belong to the same response regulator family as B. subtilis DesR (16). BC5440 encodes a response regulator probably involved in the regulation of enzymes with murein hydrolase activity (like regulators in the LytR/AlgR family) (24).…”

Section: Discussionmentioning

confidence: 99%

Identification of Bacillus cereus Genes Specifically Expressed during Growth at Low Temperatures

Brillard

Jéhanno

Dargaignaratz

et al. 2010

Appl Environ Microbiol

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The mechanisms involved in the ability of Bacillus cereus to multiply at low temperatures were investigated. It was assumed that many genes involved in cold acclimation would be upregulated at low temperatures. Recombinase-based in vivo expression technology (IVET) was adapted to the detection of the transient activation of B. cereus promoters during growth at 10°C. Four independent screenings of a promoter library from type strain ATCC 14579 were performed, and 17 clones were isolated. They corresponded to 17 promoter regions that displayed reproducibly elevated expression at 10°C relative to expression at 30°C. This analysis revealed several genes that may be important for B. cereus to grow successfully under the restrictive conditions of cold habitats. Among them, a locus corresponding to open reading frames BC5402 to BC5398, harboring a lipase-encoding gene and a putative transcriptional regulator, was identified three times. While a mutation in the putative regulator-encoding gene did not cause any particular phenotype, a mutant deficient in the lipase-encoding gene showed reduced growth abilities at low temperatures compared with the parental strain. The mutant did not change its fatty acid profiles in the same way as the wild type when grown at 12°C instead of 37°C. This study demonstrates the feasibility of a promoter trap strategy for identifying cold-induced genes. It outlines a first picture of the different processes involved in B. cereus cold acclimation.The food-borne disease agent Bacillus cereus is an endospore-forming bacterium belonging to the B. cereus group (B. cereus sensu lato). B. cereus sensu lato has recently been divided into seven major phylogenetic groups (I to VII) with clear-cut differences in growth temperature ranges, suggesting that the genetic structure corresponds to "thermotypes" and showing the emergence of multiple psychrotrophic groups within B. cereus sensu lato (26). Temperature adaptation has thus presumably played a major role in B. cereus evolution. B. cereus is also a human pathogen, causing local and systemic infections. Most outbreaks of food-borne poisoning have been caused by mesophilic strains (26) that can grow at temperatures as low as 10°C. This characteristic enables initially relatively low levels of B. cereus in foods to increase greatly under commonly reported suboptimal refrigeration conditions (20). Understanding the ability of B. cereus to grow at low temperatures will help to control multiplication in refrigerated food and prevent outbreaks of food-borne poisoning.At low temperatures, bacteria undergo various modifications in cellular physiology, with effects such as decreased membrane fluidity and inefficient folding of proteins and secondary structures of RNA and DNA (43). Bacterial responses can be divided into low-temperature responses (or acclimation, also called low-temperature adaptation) and cold shock responses (43). Both types of responses include a vast array of structural and physiological adjustments to cope with the reduction in biochemic...

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“…To map the occurrence of RsbK and RsbY homologues in other species, a similar approach was followed as described previously (de Been et al, 2006(de Been et al, , 2010. This approach included a straightforward BLAST search with the HK phosphotransferase (RsbK) and REC (RsbK and RsbY) domain sequences.…”

Section: Rsbk-type Hybrid Kinases Occur In a Wide Variety Of Bacteriamentioning

confidence: 99%

Novel σ B regulation modules of Gram-positive bacteria involve the use of complex hybrid histidine kinases

et al. 2011

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A common bacterial strategy to cope with stressful conditions is the activation of alternative sigma factors that control specific regulons enabling targeted responses. In the human pathogen Bacillus cereus, activation of the major stress-responsive sigma factor s B is controlled by a signalling route that involves the multi-sensor hybrid histidine kinase RsbK. RsbK-type kinases are not restricted to the B. cereus group, but occur in a wide variety of other bacterial species, including members of the the low-GC Gram-positive genera Geobacillus and Paenibacillus as well as the high-GC actinobacteria. Genome context and protein sequence analyses of 118 RsbK homologues revealed extreme variability in N-terminal sensory as well as C-terminal regulatory domains and suggested that RsbK-type kinases are subject to complex fine-tuning systems, including sensitization and desensitization via methylation and demethylation within the helical domain preceding the H-box. The RsbK-mediated stress-responsive sigma factor activation mechanism that has evolved in B. cereus and the other species differs markedly from the extensively studied and highly conserved RsbRST-mediated s B activation route found in Bacillus subtilis and other low-GC Gram-positive bacteria. Implications for future research on sigma factor control mechanisms are presented and current knowledge gaps are briefly discussed.

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Comparative analysis of two-component signal transduction systems of Bacillus cereus, Bacillus thuringiensis and Bacillus anthracis

Cited by 53 publications

References 89 publications

Comparative Genomic Analysis of Two-Component Signal Transduction Systems in Probiotic Lactobacillus casei

Comparative Genomic Analysis of Two-Component Signal Transduction Systems in Probiotic Lactobacillus casei

Identification of Bacillus cereus Genes Specifically Expressed during Growth at Low Temperatures

Novel σ B regulation modules of Gram-positive bacteria involve the use of complex hybrid histidine kinases

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