Comparative analysis of two porcine kidney decellularization methods for maintenance of functional vascular architectures

Zambon, João Paulo; Ko, In Kap; Abolbashari, Mehran; Huling, Jennifer; Clouse, Cara; Kim, Tae Hyoung; Smith, Charesa J.; Atala, Anthony; Yoo, James J.

doi:10.1016/j.actbio.2018.06.004

Cited by 57 publications

(38 citation statements)

References 21 publications

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“…Zambon et al . addressed a further common concern regarding decellularization, which is the preservation of the vascular structures, by showing the disruption of the glomerular microarchitecture following the perfusion of detergents through the renal artery, and he modified the flow rate, detergent concentration and decellularization time to solve this problem. The kidney contains more than 26 types of cells organized in a complicated structure consisting of thousands of nephrons .…”

Section: Kidneymentioning

confidence: 99%

Strategies based on organ decellularization and recellularization

et al. 2019

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Summary Transplantation is the only curative treatment option available for patients suffering from end‐stage organ failure, improving their quality of life and long‐term survival. However, because of organ scarcity, only a small number of these patients actually benefit from transplantation. Alternative treatment options are needed to address this problem. The technique of whole‐organ decellularization and recellularization has attracted increasing attention in the last decade. Decellularization includes the removal of all cellular components from an organ, while simultaneously preserving the micro and macro anatomy of the extracellular matrix. These bioscaffolds are subsequently repopulated with patient‐derived cells, thus constructing a personalized neo‐organ and ideally eliminating the need for immunosuppression. However, crucial problems have not yet been satisfyingly addressed and remain to be resolved, such as organ and cell sources. In this review, we focus on the actual state of organ de‐ and recellularization, as well as the problems and future challenges.

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Section: Kidneymentioning

confidence: 99%

Strategies based on organ decellularization and recellularization

et al. 2019

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“…The authors reported defects in the vascular cast and damaged glomerular structures for SDS + DNase treated tissues while Triton X‐100 + SDS treated tissues showed similar morphology with native kidney ( Figure ). [ 52 ]…”

Section: Characterization Methods For Decellularized Tissuesmentioning

confidence: 99%

“…[ 51 ] When DNase was combined with SDS, microarchitecture is reported to be damaged. [ 52 ] Chelating agents, such as ethylenediaminetetraacetic acid (EDTA), disrupt cell adhesion, and generally are combined with other decellularization agents to improve decellularization. The addition of EDTA to detergents increases the effectiveness of decellularization but can damage the ECM architecture.…”

Section: Decellularization Processesmentioning

confidence: 99%

Designing Decellularized Extracellular Matrix‐Based Bioinks for 3D Bioprinting

Abaci

Guvendiren

2020

Adv Healthcare Materials

129

101

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3D bioprinting is an emerging technology to fabricate tissues and organs by precisely positioning cells into 3D structures using printable cell‐laden formulations known as bioinks. Various bioinks are utilized in 3D bioprinting applications; however, developing the perfect bioink to fabricate constructs with biomimetic microenvironment and mechanical properties that are similar to native tissues is a challenging task. In recent years, decellularized extracellular matrix (dECM)‐based bioinks have received an increasing attention in 3D bioprinting applications, since they are derived from native tissues and possess unique, complex tissue‐specific biochemical properties. This review focuses on designing dECM‐based bioinks for tissue and organ bioprinting, including commonly used decellularization and decellularized tissue characterization methods, bioink formulation and characterization, applications of dECM‐based bioinks, and most recent advancements in dECM‐based bioink design.

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“…A number of imaging modalities have been used to visualize the vascular tree in bioengineered organs. These include contrast‐enhanced CT, 9,13‐16 MRI, 17 Doppler US for in vivo follow‐up, 15 fluorescence microscopy, 18,19 and fluoroscopic angiography 10,20,21 …”

Section: Introductionmentioning

confidence: 99%

“…These include contrast-enhanced CT, 9,[13][14][15][16] MRI, 17 Doppler US for in vivo follow-up, 15 fluorescence microscopy, 18,19 and fluoroscopic angiography. 10,20,21 However, none of these studies quantified changes in vascular permeability that may occur during perfusion decellularization. Moreover, demonstration of vascular patency in most studies was limited to visualization of the arterial tree.…”

mentioning

confidence: 99%

Flow‐controlled fluoroscopic angiography for the assessment of vascular integrity in bioengineered kidneys

et al. 2020

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Perfusion decellularization has been proposed as a promising method for generating nonimmunogenic organs from allogeneic or xenogeneic donors. Several imaging modalities have been used to assess vascular integrity in bioengineered organs with no consistency in the methodology used. Here, we studied the use of fluoroscopic angiography performed under controlled flow conditions for vascular integrity assessment in bioengineered kidneys. Porcine kidneys underwent ex vivo angiography before and after perfusion decellularization. Arterial and venous patencies were defined as visualization of contrast medium (CM) in distal capillaries and renal vein, respectively. Changes in vascular permeability were visualized and quantified. No differences in patency were detected in decellularized kidneys compared with native kidneys. However, focal parenchymal opacities and significant delay in CM clearance were detected in decellularized kidneys, indicating increased permeability. Biopsy‐induced leakage was visualized in both groups, with digital subtraction angiography revealing minimal CM leakage earlier than nonsubtracted fluoroscopy. In summary, quantitative assessment of vascular permeability should be coupled with patency when studying the effect of perfusion decellularization on kidney vasculature. Flow‐controlled angiography should be considered as the method of choice for vascular assessment in bioengineered kidneys. Adopting this methodology for organs premodified ex vivo under normothermic machine perfusion settings is also suggested.

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Comparative analysis of two porcine kidney decellularization methods for maintenance of functional vascular architectures

Cited by 57 publications

References 21 publications

Strategies based on organ decellularization and recellularization

Strategies based on organ decellularization and recellularization

Designing Decellularized Extracellular Matrix‐Based Bioinks for 3D Bioprinting

Flow‐controlled fluoroscopic angiography for the assessment of vascular integrity in bioengineered kidneys

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