Comparative analysis of zebrafish nos2a and nos2b genes

Lepiller, Sandrine; Franche, Nathalie; Solary, Éric; Chluba, Johanna; Laurens, Véronique

doi:10.1016/j.gene.2009.05.016

Cited by 66 publications

(44 citation statements)

References 42 publications

(42 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, they concluded that out of the four Nos variants that resulted from the teleost-specific genome duplication, two of them were lost, leaving one variant of Nos1 and one of Nos2 in the early teleosts. As opposed to many higher teleosts that have lost their Nos2 variant (2), the Cypriniformes lineage (to which carps belong) has retained Nos2 and even experienced an additional duplication of this gene, giving rise to Nos2A and Nos2B in zebrafish (46). In accordance with this, we found sequences for Nos1, Nos2A, and Nos2B in crucian carp.…”

Section: R471 No Metabolites In Anoxic Crucian Carpsupporting

confidence: 81%

Dramatic increase of nitrite levels in hearts of anoxia-exposed crucian carp supporting a role in cardioprotection

Sandvik

Nilsson

Frank

2012

American Journal of Physiology-Regulatory, Integrative and Comp

View full text Add to dashboard Cite

Sandvik GK, Nilsson GE, Jensen FB. Dramatic increase of nitrite levels in hearts of anoxia-exposed crucian carp supporting a role in cardioprotection. Am J Physiol Regul Integr Comp Physiol 302: R468 -R477, 2012. First published November 30, 2011 doi:10.1152/ajpregu.00538.2011 Ϫ ) functions as an important nitric oxide (NO) donor under hypoxic conditions. Both nitrite and NO have been found to protect the mammalian heart and other tissues against ischemia (anoxia)-reoxygenation injury by interacting with mitochondrial electron transport complexes and limiting the generation of reactive oxygen species upon reoxygenation. The crucian carp naturally survives extended periods without oxygen in an active state, which has made it a model for studying how evolution has solved the problems of anoxic survival. We investigated the role of nitrite and NO in the anoxia tolerance of this fish by measuring NO metabolites in normoxic, anoxic, and reoxygenated crucian carp. We also cloned and sequenced crucian carp NO synthase variants and quantified their mRNA levels in several tissues in normoxia and anoxia. Despite falling levels of blood plasma nitrite, the crucian carp showed massive increases in nitrite, S-nitrosothiols (SNO), and ironnitrosyl (FeNO) compounds in anoxic heart tissue. NO 2 Ϫ levels were maintained in anoxic brain, liver, and gill tissues, whereas SNO and FeNO increased in a tissue-specific manner. Reoxygenation reestablished normoxic values. We conclude that NO 2 Ϫ is shifted into the tissues where it acts as NO donor during anoxia, inducing cytoprotection under anoxia/reoxygenation. This can be especially important in the crucian carp heart, which maintains output in anoxia. NO 2 Ϫ is currently tested as a therapeutic drug against reperfusion damage of ischemic hearts, and the present study provides evolutionary precedent for such an approach. nitric oxide; S-nitrosothiols; anoxia/reoxygenation; cytoprotection; nitric oxide synthase NITRIC OXIDE (NO) is an important regulator of many biological functions in vertebrates. In normoxic conditions, NO is produced from L-arginine and O 2 by NO synthases (NOS). The classical role is in vasodilation, where NO binds to the heme of the guanylyl cyclase in smooth muscle cells, leading to a rise in cGMP and subsequent muscle relaxation (35). NO also binds to heme groups of other proteins and is involved in reactions with thiols and secondary amines of different proteins, leading to formation of iron-nitrosyls (FeNO), S-nitrosothiols (SNO), and N-nitrosamines (NNO), respectively (26). Through formation of FeNO and SNO, NO can activate and inactivate enzymes or modify protein function (17,73). NO is inactivated by oxidation to nitrate (NO 3 Ϫ ) in reaction with oxygenated hemoglobin and myoglobin, and it is metabolized to nitrite (NO 2 Ϫ ) in reaction with O 2 (48). Nitrite was previously considered an inert end product of this oxidation, but nitrite can be reduced back to NO, and it has become clear that nitrite functions as a NO storage pool that is activated by va...

show abstract

Section: R471 No Metabolites In Anoxic Crucian Carpsupporting

confidence: 81%

Dramatic increase of nitrite levels in hearts of anoxia-exposed crucian carp supporting a role in cardioprotection

Sandvik

Nilsson

Frank

2012

American Journal of Physiology-Regulatory, Integrative and Comp

View full text Add to dashboard Cite

show abstract

“…In some cases, duplicated genes have complementary roles, sharing multiple functions of the ancestral gene (49). The dual zebrafish paralogues of ␥-synuclein show distinct temporal and spatial expression patterns; this may have allowed segregation of neural and non-neural functions of ␥-synuclein into separate proteins and provided evolutionary pressure to retain both genes.…”

Section: Discussionmentioning

confidence: 99%

Hypokinesia and Reduced Dopamine Levels in Zebrafish Lacking β- and γ1-Synucleins

Milanese

Sager

Bai

et al. 2012

Journal of Biological Chemistry

View full text Add to dashboard Cite

Background: Synucleins are implicated in Parkinson disease pathogenesis, but their functions are incompletely understood. Results: Zebrafish lacking ␤-and ␥1-synucleins showed hypokinesia and decreased dopamine levels. These abnormalities were rescued by human ␣-synuclein. Conclusion: Synucleins are necessary for spontaneous movement and dopaminergic function. Significance: A key functional role for synucleins in dopamine neurons may be relevant to Parkinson disease pathogenesis.

show abstract

“…After tail transection in zebrafish larvae, iNOS gene expression increases significantly in the regenerative site and in skin cells (Lepiller et al 2009) and the use of NOS or specific guanilate cyclase inhibitors reduces head regeneration in Hydra, whereas an NO donor has the opposite effect (Colasanti et al 2009). Fig.…”

Section: Discussionmentioning

confidence: 99%

Tail regenerative capacity and iNOS immunolocalization in Xenopus laevis tadpoles

Franchini

Bertolotti

2011

Cell Tissue Res

View full text Add to dashboard Cite

The morphology and the immuno-distribution of the inducible isoform of nitric oxide synthase (iNOS) have been examined in regenerating tails from differently aged Xenopus laevis larvae. By comparing stage-50 and stage-55/56 tadpoles, various morphological aspects and immunoreactivity to anti-iNOS antibody in terms of the number and duration of positive cells have been demonstrated in the regenerating buds. Unlike in stage-50 larvae, the extent of responses to tail amputation in older larvae is more dependent on the individual tadpole and a high percentage (70%-80%) of malformed tails has been seen. The findings indicate that the decline in the efficiency of Xenopus tail regeneration is driven by differences in the inflammatory responses and in the involvement of nitric oxide. This molecule is induced and required for normal tail regeneration, whereas in excess, it is probably associated with progressive loss in the regeneration capability.

show abstract

Comparative analysis of zebrafish nos2a and nos2b genes

Cited by 66 publications

References 42 publications

Dramatic increase of nitrite levels in hearts of anoxia-exposed crucian carp supporting a role in cardioprotection

Dramatic increase of nitrite levels in hearts of anoxia-exposed crucian carp supporting a role in cardioprotection

Hypokinesia and Reduced Dopamine Levels in Zebrafish Lacking β- and γ1-Synucleins

Tail regenerative capacity and iNOS immunolocalization in Xenopus laevis tadpoles

Contact Info

Product

Resources

About